Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents
Primary human mesenchymal stem cells (hMSCs) have attracted much attention in regenerative medicine, where stem cell culture currently requires xeno-free (xenogeneic component-free) systems for both media and scaffolds. Herein, we propose plant-derived, sulfated cellulose nanofibers (S-CNFs) with di...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Carbohydrate Polymer Technologies and Applications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925002713 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849321206908452864 |
|---|---|
| author | Ritomo Kai Mayumi Hatakeyama Shinichiro Iwamoto Takuya Kitaoka |
| author_facet | Ritomo Kai Mayumi Hatakeyama Shinichiro Iwamoto Takuya Kitaoka |
| author_sort | Ritomo Kai |
| collection | DOAJ |
| description | Primary human mesenchymal stem cells (hMSCs) have attracted much attention in regenerative medicine, where stem cell culture currently requires xeno-free (xenogeneic component-free) systems for both media and scaffolds. Herein, we propose plant-derived, sulfated cellulose nanofibers (S-CNFs) with different sulfate contents as a novel xeno-free scaffold. Primary and immortalized hMSCs were cultured on S-CNF scaffolds with different sulfate contents (0–1.69 mmol g–1) under serum-free conditions. The cell proliferation behavior was sensitive to the sulfate content, although the original CNFs did not contribute to cell adhesion. In particular, S-CNF scaffolds with sulfate contents of 0.31–0.47 mmol g–1 demonstrated more efficient cell growth than a standard polystyrene substrate and comparable cell growth to animal-derived type I collagen. The engineered S-CNF scaffolds with the optimal sulfate content promoted the initial cell adhesion and proliferation of primary hMSCs by facilitating the formation of focal adhesions. In addition, these scaffolds improved the stability and efficacy of growth factors in serum-free environments, potentially contributing to their functional preservation in xeno-free culture systems. Our strategy of using S-CNFs as a new medical modality provides new insights into the development of xeno-free culture systems in cell culture engineering. |
| format | Article |
| id | doaj-art-edf82278eaad4aaab6b95c865f1e4ad9 |
| institution | Kabale University |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-edf82278eaad4aaab6b95c865f1e4ad92025-08-20T03:49:49ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-09-011110093010.1016/j.carpta.2025.100930Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contentsRitomo Kai0Mayumi Hatakeyama1Shinichiro Iwamoto2Takuya Kitaoka3Department of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, JapanDepartment of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, JapanYokogawa Electric Corporation, Musashino, Tokyo 180-8750, JapanDepartment of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan; Corresponding author.Primary human mesenchymal stem cells (hMSCs) have attracted much attention in regenerative medicine, where stem cell culture currently requires xeno-free (xenogeneic component-free) systems for both media and scaffolds. Herein, we propose plant-derived, sulfated cellulose nanofibers (S-CNFs) with different sulfate contents as a novel xeno-free scaffold. Primary and immortalized hMSCs were cultured on S-CNF scaffolds with different sulfate contents (0–1.69 mmol g–1) under serum-free conditions. The cell proliferation behavior was sensitive to the sulfate content, although the original CNFs did not contribute to cell adhesion. In particular, S-CNF scaffolds with sulfate contents of 0.31–0.47 mmol g–1 demonstrated more efficient cell growth than a standard polystyrene substrate and comparable cell growth to animal-derived type I collagen. The engineered S-CNF scaffolds with the optimal sulfate content promoted the initial cell adhesion and proliferation of primary hMSCs by facilitating the formation of focal adhesions. In addition, these scaffolds improved the stability and efficacy of growth factors in serum-free environments, potentially contributing to their functional preservation in xeno-free culture systems. Our strategy of using S-CNFs as a new medical modality provides new insights into the development of xeno-free culture systems in cell culture engineering.http://www.sciencedirect.com/science/article/pii/S2666893925002713Cellulose nanofiberSulfate esterificationXeno-free scaffoldCell adhesion mechanismStem cell culture |
| spellingShingle | Ritomo Kai Mayumi Hatakeyama Shinichiro Iwamoto Takuya Kitaoka Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents Carbohydrate Polymer Technologies and Applications Cellulose nanofiber Sulfate esterification Xeno-free scaffold Cell adhesion mechanism Stem cell culture |
| title | Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents |
| title_full | Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents |
| title_fullStr | Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents |
| title_full_unstemmed | Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents |
| title_short | Adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents |
| title_sort | adhesion and proliferation behavior of primary human mesenchymal stem cells on sulfated cellulose nanofiber scaffolds with different sulfate contents |
| topic | Cellulose nanofiber Sulfate esterification Xeno-free scaffold Cell adhesion mechanism Stem cell culture |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925002713 |
| work_keys_str_mv | AT ritomokai adhesionandproliferationbehaviorofprimaryhumanmesenchymalstemcellsonsulfatedcellulosenanofiberscaffoldswithdifferentsulfatecontents AT mayumihatakeyama adhesionandproliferationbehaviorofprimaryhumanmesenchymalstemcellsonsulfatedcellulosenanofiberscaffoldswithdifferentsulfatecontents AT shinichiroiwamoto adhesionandproliferationbehaviorofprimaryhumanmesenchymalstemcellsonsulfatedcellulosenanofiberscaffoldswithdifferentsulfatecontents AT takuyakitaoka adhesionandproliferationbehaviorofprimaryhumanmesenchymalstemcellsonsulfatedcellulosenanofiberscaffoldswithdifferentsulfatecontents |