Crystal structure and catalytic mechanism of PL35 family glycosaminoglycan lyases with an ultrabroad substrate spectrum

Crystal structure and catalytic mechanism of PL35 family glycosaminoglycan lyases with an ultrabroad substrate spectrum

Recently, a new class of glycosaminoglycan (GAG) lyases (GAGases) belonging to PL35 family has been discovered with an ultrabroad substrate spectrum that can degrade three types of uronic acid-containing GAGs (hyaluronic acid, chondroitin sulfate and heparan sulfate) or even alginate. In this study,...

Full description

Saved in:

Bibliographic Details
Main Authors:	Lin Wei, Hai-Yan Cao, Ruyi Zou, Min Du, Qingdong Zhang, Danrong Lu, Xiangyu Xu, Yingying Xu, Wenshuang Wang, Xiu-Lan Chen, Yu-Zhong Zhang, Fuchuan Li
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2025-05-01
Series:	eLife
Subjects:	glycosaminoglycan lyases polysaccharide lyase 35 family crystal structure catalytic mechanism
Online Access:	https://elifesciences.org/articles/102422
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

Utilization of glycosaminoglycans by the human gut microbiota: participating bacteria and their enzymatic machineries
by: Parkash Singh Rawat, et al.
Published: (2022-12-01)

Characterisation of High Alkaline-Tolerant Novel Ulvan Lyase from <i>Pseudoalteromonas agarivorans</i>: Potential Applications of Enzyme Derived Oligo-Ulvan as Anti-Diabetic Agent
by: Navindu Dinara Gajanayaka, et al.
Published: (2024-12-01)

Alginate-Degrading Modes, Oligosaccharide-Yielding Properties, and Potential Applications of a Novel Bacterial Multifunctional Enzyme, Aly16-1
by: Lianghuan Zeng, et al.
Published: (2024-11-01)

Multi-Functional Alginate Lyase <i>AlgVR7</i> from <i>Vibrio rumoiensis</i>: Structural Insights and Catalytic Mechanisms
by: Zhe Huang, et al.
Published: (2025-03-01)

Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate
by: Xiao Liu, et al.
Published: (2024-12-01)

Effect of potent inhibitors of phenylalanine ammonia-lyase and PVP on in vitro morphogenesis of Fagopyrum tataricum
by: Reneé Pérez-Pérez, et al.
Published: (2025-04-01)

Degradation of Natural <i>Undaria pinnatifida</i> into Unsaturated Guluronic Acid Oligosaccharides by a Single Alginate Lyase
by: Hui Wang, et al.
Published: (2024-10-01)

Exploring the Catalytic Mechanisms of a Newly Identified Salt-Activated Alginate Lyase from <i>Pseudoalteromonas carrageenovora</i> ASY5
by: Xiaoyan Zhuang, et al.
Published: (2025-06-01)

Transcriptomic response of the picoalga Pelagomonas calceolata to nitrogen availability: new insights into cyanate lyase function
by: Nina Guérin, et al.
Published: (2025-05-01)

A PL1 family pectate lyase CP966_RS08110 gene was the pathogenic factor of Streptomyces galilaeus 5T-1 causing potato common scab
by: Cuiwen Zhang, et al.
Published: (2024-11-01)

Pyruvate formate lyase regulates fermentation metabolism and virulence of Streptococcus suis
by: Qingying Fan, et al.
Published: (2025-12-01)

Improvement of Catalytic Activity and Thermostability of Alginate Lyase VxAly7B-CM via Rational Computational Design Strategies
by: Xin Ma, et al.
Published: (2025-05-01)

Cystathionine γ-lyase downregulation promotes liver injury and necroptosis through reprogramming of methionine cycle
by: Cichun Wu, et al.
Published: (2025-12-01)

Application of marine bacterial alginate lyases in wastewater treatment, biofilm removal and green technology
by: M.U.K. Anjung, et al.
Published: (2025-07-01)

New Insights on the Glyphosate-Degrading Enzymes C-P Lyase and Glyphosate Oxidoreductase Based on Bioinformatics
by: Marina Giannakara, et al.
Published: (2024-10-01)

Expression, Purification, and Characterisation of Recombinant Alginate Lyase (<i>Flammeovirga</i> AL2) for the Bioconversion of Alginate into Alginate Oligosaccharides
by: Coleen E. Grobler, et al.
Published: (2024-11-01)

Influence of sex hormones on DNA content and cystathionine-γ-lyase expression in rat myocardium
by: A. V. Melnik, et al.
Published: (2017-12-01)

Cloning, expression and sequence analysis of a phenylalanine ammonia lyase gene BaPAL from Chinese kale (Brassica alboglabra)
by: JIANG Ming, et al.
Published: (2010-03-01)

Alginate lyase immobilized Chlamydomonas algae microrobots: minimally invasive therapy for biofilm penetration and eradication
by: Xiaoting Zhang, et al.
Published: (2025-06-01)

Allosteric activators of cystathionine γ lyase to augment endogenous hydrogen sulfide and inhibit pathologic calcification
by: Sonia Nasi, et al.
Published: (2025-09-01)

Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em>
by: Linjing Zhang, et al.
Published: (2024-08-01)

Identification, Whole-Genome Sequencing and Analysis of Vibrio alginolyticus S10, a Strain Producing Alginate Lyase
by: LIU Fang, SHU Zhiqiang, WANG Gongming, JING Yuexin, ZHAO Yunping, XU Yingjiang, JIAO Chunna, ZHANG Jian
Published: (2024-11-01)

A comparative study of the efficacy of alginate lyases in the presence of metal ions elevated in the cystic fibrosis lung milieu
by: Neetu, et al.
Published: (2024-12-01)

Screening and identification of marine alginate lyase-producing bacteria and optimization of enzyme production conditions
by: HU Mengdi, LI Yaozu, ZHANG Xiaoyong, MO Meiqing, GAO Xiangyang
Published: (2025-04-01)

Novel sphingosine-1-phosphate lyase mutation causes multisystemic diseases: case report
by: Gönül Büyükyılmaz, et al.
Published: (2023-12-01)

Screening, Optimization of Fermentation Conditions and Evaluation of Sargassum-degrading Effect of Exiguobacterium mexicanum MAL070 Producing Alginate Lyase
by: Shuang LUO, et al.
Published: (2025-08-01)

Insights into putative alginate lyases from epipelagic and mesopelagic communities of the global ocean
by: Mariana Lozada, et al.
Published: (2025-03-01)

Isolation and identification of an alginate lyase-producing marine bacterium strain and its enzymological characteristics
by: TANG Haiqing, et al.
Published: (2013-07-01)

The Identification and Characterization of a Novel Alginate Lyase from <i>Mesonia hitae</i> R32 Exhibiting High Thermal Stability and Potent Antioxidant Oligosaccharide Production
by: Yongshang Ye, et al.
Published: (2025-04-01)

Safety evaluation of a food enzyme containing endo‐polygalacturonase and pectin lyase activities from the non‐genetically modified Aspergillus tubingensis strain NZYM‐PE
by: EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), et al.
Published: (2023-07-01)

One-Pot Synthesis of β-Alanine from Fumaric Acid via an Efficient Dual-Enzyme Cascade Biotransformation
by: Zifu Ni, et al.
Published: (2024-12-01)

The Catalytic Mechanism of Key Enzymes Involved in the Synthesis of Ergothioneine in <i>Lentinula edodes</i>
by: Zheng Li, et al.
Published: (2024-12-01)

Expression of Astragalus membranaceus phenylalanine ammonia-lyase gene in Pichia pastoris
by: Zhang Jianhui, et al.
Published: (2014-01-01)

The impact of salicylic acid on some physiological responses of Artemisia aucheri Boiss. under in vitro drought stress
by: Jalil ABBASPOUR, et al.
Published: (2016-10-01)

The Pathogenetic Basis of the Action of Bempedoic Acid
by: A. S. Petrosyan, et al.
Published: (2023-01-01)

Protein acetylation affects acetate metabolism, motility and acid stress response in Escherichia coli
by: Sara Castaño‐Cerezo, et al.
Published: (2014-11-01)

Purification, characterization, and anti-cancer activity of methionine gamma-lyase from a native strain of Pseudomonas mosselii for human cancer treatment
by: Matin Nasirian, et al.
Published: (2025-07-01)

Cellulophaga algicola alginate lyase and Pseudomonas aeruginosa Psl glycoside hydrolase inhibit biofilm formation by Pseudomonas aeruginosa CF2843 on three-dimensional aggregates of lung epithelial cells
by: Neetu, et al.
Published: (2025-06-01)

Production and purification of methionine gamma-lyase from Iranian soil mulds: investigation of physicochemical properties and anticancer effects
by: Matin Nasirian, et al.
Published: (2025-08-01)

Development of a Dual-Stage CIM<sup>®</sup> CDI Reactor with Immobilized Glucuronan Lyases and Laccases for Sustainable Synthesis of Antioxidant Phenolized Oligoglucuronan
by: Xiaoyang Hou, et al.
Published: (2024-11-01)