Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens
The effectiveness of the bacteria antagonist Pseudomonas fluorescens to control green mold caused by Penicillium digitatum on oranges (Citrus sinensis Osbeck, cv. Jincheng) and the possible modes of action were evaluated. Whether in vitro or in vivo, treatments with cell-free autoclaved cultures or...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2018/2910481 |
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| author | Zhirong Wang Mengyao Jiang Kewei Chen Kaituo Wang Muying Du Zsolt Zalán Ferenc Hegyi Jianquan Kan |
| author_facet | Zhirong Wang Mengyao Jiang Kewei Chen Kaituo Wang Muying Du Zsolt Zalán Ferenc Hegyi Jianquan Kan |
| author_sort | Zhirong Wang |
| collection | DOAJ |
| description | The effectiveness of the bacteria antagonist Pseudomonas fluorescens to control green mold caused by Penicillium digitatum on oranges (Citrus sinensis Osbeck, cv. Jincheng) and the possible modes of action were evaluated. Whether in vitro or in vivo, treatments with cell-free autoclaved cultures or culture filtrate had limited capacity to suppress P. digitatum, while P. digitatum was significantly inhibited by bacterial fluid (P. fluorescens in the nutrient broth liquid medium) and bacterial suspension (P. fluorescens in sterile distilled water) with living cells. There was a positive relationship between the concentration of P. fluorescens in bacterial suspension and its biological efficacy. In addition, P. fluorescens was effective when applied preventatively but not when applied curatively. In the inoculated wounds, the population of P. fluorescens was an approximately 28- and 34-fold increase after being incubated at 20°C for 8 d and at 4°C for 16 d, respectively, and P. digitatum could effectively stimulate the growth and reproduction of P. fluorescens. Moreover, P. fluorescens was able to inhibit spore germination and germ tube elongation of P. digitatum as well as induce resistance on citrus peel by increasing the chitinase (CHI) activity and advancing the activities peaks of β-1,3-glucanase (GLU), peroxidase (POD), and phenylalanine ammonia lyase (PAL). All of these results support the potential application of P. fluorescens against green mold on postharvest citrus. |
| format | Article |
| id | doaj-art-edde8a67a6a04f9eaba222f50337cb8f |
| institution | DOAJ |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-edde8a67a6a04f9eaba222f50337cb8f2025-08-20T03:20:44ZengWileyJournal of Food Quality0146-94281745-45572018-01-01201810.1155/2018/29104812910481Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescensZhirong Wang0Mengyao Jiang1Kewei Chen2Kaituo Wang3Muying Du4Zsolt Zalán5Ferenc Hegyi6Jianquan Kan7College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, ChinaCollege of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, ChinaCollege of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, ChinaChinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, ChinaCollege of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, ChinaChinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, ChinaChinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, ChinaCollege of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, ChinaThe effectiveness of the bacteria antagonist Pseudomonas fluorescens to control green mold caused by Penicillium digitatum on oranges (Citrus sinensis Osbeck, cv. Jincheng) and the possible modes of action were evaluated. Whether in vitro or in vivo, treatments with cell-free autoclaved cultures or culture filtrate had limited capacity to suppress P. digitatum, while P. digitatum was significantly inhibited by bacterial fluid (P. fluorescens in the nutrient broth liquid medium) and bacterial suspension (P. fluorescens in sterile distilled water) with living cells. There was a positive relationship between the concentration of P. fluorescens in bacterial suspension and its biological efficacy. In addition, P. fluorescens was effective when applied preventatively but not when applied curatively. In the inoculated wounds, the population of P. fluorescens was an approximately 28- and 34-fold increase after being incubated at 20°C for 8 d and at 4°C for 16 d, respectively, and P. digitatum could effectively stimulate the growth and reproduction of P. fluorescens. Moreover, P. fluorescens was able to inhibit spore germination and germ tube elongation of P. digitatum as well as induce resistance on citrus peel by increasing the chitinase (CHI) activity and advancing the activities peaks of β-1,3-glucanase (GLU), peroxidase (POD), and phenylalanine ammonia lyase (PAL). All of these results support the potential application of P. fluorescens against green mold on postharvest citrus.http://dx.doi.org/10.1155/2018/2910481 |
| spellingShingle | Zhirong Wang Mengyao Jiang Kewei Chen Kaituo Wang Muying Du Zsolt Zalán Ferenc Hegyi Jianquan Kan Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens Journal of Food Quality |
| title | Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens |
| title_full | Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens |
| title_fullStr | Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens |
| title_full_unstemmed | Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens |
| title_short | Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens |
| title_sort | biocontrol of penicillium digitatum on postharvest citrus fruits by pseudomonas fluorescens |
| url | http://dx.doi.org/10.1155/2018/2910481 |
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