Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity
This study aimed to evaluate the antibacterial activity against multi-drug-resistant strains carrying efflux pumps and assess their toxicity on Drosophila melanogaster and Aedes aegypti models. Microdilution tests in broth were performed to determine the Minimum Inhibitory Concentration (MIC). The e...
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Elsevier
2025-06-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750025000356 |
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| author | Gildênia Alves de Araújo Cícera Datiane de Morais Oliveira Tintino Raimundo Luíz Silva Pereira Isaac Moura Araújo Cícera Laura Roque Paulo João Arthur de Oliveira Borges Ewerton Yago de Sousa Rodrigues Ângella Eduarda da Silva Francisco Assis Bezerra da Cunha Zildene de Sousa Silveira Nair Silva Macedo Henrique Douglas Melo Coutinho José Maria Barbosa Filho Daniela Maria do Amaral Ferraz Navarro Francisco Roberto de Azevedo Saulo Relison Tintino |
| author_facet | Gildênia Alves de Araújo Cícera Datiane de Morais Oliveira Tintino Raimundo Luíz Silva Pereira Isaac Moura Araújo Cícera Laura Roque Paulo João Arthur de Oliveira Borges Ewerton Yago de Sousa Rodrigues Ângella Eduarda da Silva Francisco Assis Bezerra da Cunha Zildene de Sousa Silveira Nair Silva Macedo Henrique Douglas Melo Coutinho José Maria Barbosa Filho Daniela Maria do Amaral Ferraz Navarro Francisco Roberto de Azevedo Saulo Relison Tintino |
| author_sort | Gildênia Alves de Araújo |
| collection | DOAJ |
| description | This study aimed to evaluate the antibacterial activity against multi-drug-resistant strains carrying efflux pumps and assess their toxicity on Drosophila melanogaster and Aedes aegypti models. Microdilution tests in broth were performed to determine the Minimum Inhibitory Concentration (MIC). The efflux pump inhibition was evaluated by analyzing the reduction in antibiotic MIC and Ethidium Bromide (EtBr) MIC when combined with the products. Mortality assay and negative geotaxis were conducted on D. melanogaster specimens, and insecticidal activity assays were performed on A. aegypti larvae. Only geraniol reduced the antibiotic MIC when combined, reducing from 64 µg/mL to 16 µg/mL in the 1199B strain of S. aureus. When combined with EtBr, both geraniol and citral reduced EtBr MIC, with geraniol decreasing from 64 µg/mL to 16 µg/mL and citral decreasing from 64 µg/mL to 32 µg/mL. Regarding the S. aureus K2068 strain, geraniol reduced the antibiotic MIC from 16 µg/mL to 8 µg/mL, and citral reduced it from 16 µg/mL to 4 µg/mL. In combination with EtBr, all monoterpenes reduced MIC from 64 µg/mL to 32 µg/mL. Both products exhibited toxicity in D. melanogaster; however, citral showed higher toxicity with a precisely determined LC50 of 2.478 μL. As for the insecticidal action on A. aegypti, both products demonstrated toxicity with cumulative effects and dose-dependent mortality. |
| format | Article |
| id | doaj-art-edda0eda33d1404f98444f87a732e206 |
| institution | Kabale University |
| issn | 2214-7500 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Toxicology Reports |
| spelling | doaj-art-edda0eda33d1404f98444f87a732e2062025-01-26T05:03:54ZengElsevierToxicology Reports2214-75002025-06-0114101917Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicityGildênia Alves de Araújo0Cícera Datiane de Morais Oliveira Tintino1Raimundo Luíz Silva Pereira2Isaac Moura Araújo3Cícera Laura Roque Paulo4João Arthur de Oliveira Borges5Ewerton Yago de Sousa Rodrigues6Ângella Eduarda da Silva7Francisco Assis Bezerra da Cunha8Zildene de Sousa Silveira9Nair Silva Macedo10Henrique Douglas Melo Coutinho11José Maria Barbosa Filho12Daniela Maria do Amaral Ferraz Navarro13Francisco Roberto de Azevedo14Saulo Relison Tintino15Laboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, BrazilLaboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, BrazilLaboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, BrazilLaboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, BrazilLaboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, BrazilLaboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, BrazilLaboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, BrazilLaboratory of Semi-Arid Bioprospecting (LABSEMA), Department of Biological Chemistry - URCA, Crato, CE, BrazilLaboratory of Semi-Arid Bioprospecting (LABSEMA), Department of Biological Chemistry - URCA, Crato, CE, BrazilLaboratory of Semi-Arid Bioprospecting (LABSEMA), Department of Biological Chemistry - URCA, Crato, CE, BrazilLaboratory of Semi-Arid Bioprospecting (LABSEMA), Department of Biological Chemistry - URCA, Crato, CE, BrazilLaboratory of Microbiology and Molecular Biology, Department of Chemical Biology, Regional University of Cariri, Crato, Brazil; Corresponding author.Laboratory of Pharmaceutical Technology, Federal University of Paraíba — UFPB, João Pessoa 58033-455, BrazilDepartamento de Química Fundamental, Universidade Federal de Pernambuco, Recife, PE 50740-560, BrazilLaboratory of Entomology, Federal University of Cariri - UFCA, Crato, CE CEP: 63048-080, BrazilLaboratory of Semi-Arid Bioprospecting (LABSEMA), Department of Biological Chemistry - URCA, Crato, CE, BrazilThis study aimed to evaluate the antibacterial activity against multi-drug-resistant strains carrying efflux pumps and assess their toxicity on Drosophila melanogaster and Aedes aegypti models. Microdilution tests in broth were performed to determine the Minimum Inhibitory Concentration (MIC). The efflux pump inhibition was evaluated by analyzing the reduction in antibiotic MIC and Ethidium Bromide (EtBr) MIC when combined with the products. Mortality assay and negative geotaxis were conducted on D. melanogaster specimens, and insecticidal activity assays were performed on A. aegypti larvae. Only geraniol reduced the antibiotic MIC when combined, reducing from 64 µg/mL to 16 µg/mL in the 1199B strain of S. aureus. When combined with EtBr, both geraniol and citral reduced EtBr MIC, with geraniol decreasing from 64 µg/mL to 16 µg/mL and citral decreasing from 64 µg/mL to 32 µg/mL. Regarding the S. aureus K2068 strain, geraniol reduced the antibiotic MIC from 16 µg/mL to 8 µg/mL, and citral reduced it from 16 µg/mL to 4 µg/mL. In combination with EtBr, all monoterpenes reduced MIC from 64 µg/mL to 32 µg/mL. Both products exhibited toxicity in D. melanogaster; however, citral showed higher toxicity with a precisely determined LC50 of 2.478 μL. As for the insecticidal action on A. aegypti, both products demonstrated toxicity with cumulative effects and dose-dependent mortality.http://www.sciencedirect.com/science/article/pii/S2214750025000356Staphylococcus aureusEfflux pumpInsecticidal activityMonoterpenesToxicity |
| spellingShingle | Gildênia Alves de Araújo Cícera Datiane de Morais Oliveira Tintino Raimundo Luíz Silva Pereira Isaac Moura Araújo Cícera Laura Roque Paulo João Arthur de Oliveira Borges Ewerton Yago de Sousa Rodrigues Ângella Eduarda da Silva Francisco Assis Bezerra da Cunha Zildene de Sousa Silveira Nair Silva Macedo Henrique Douglas Melo Coutinho José Maria Barbosa Filho Daniela Maria do Amaral Ferraz Navarro Francisco Roberto de Azevedo Saulo Relison Tintino Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity Toxicology Reports Staphylococcus aureus Efflux pump Insecticidal activity Monoterpenes Toxicity |
| title | Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity |
| title_full | Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity |
| title_fullStr | Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity |
| title_full_unstemmed | Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity |
| title_short | Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity |
| title_sort | toxicological assessment of citral and geraniol efflux pump inhibition in staphylococcus aureus and invertebrate toxicity |
| topic | Staphylococcus aureus Efflux pump Insecticidal activity Monoterpenes Toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2214750025000356 |
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