Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline Stress
(1) Background: Global climate change is intensifying, and the vigorous development and utilization of saline–alkali land is of great significance. As an important economic aquatic species in the context of saline–alkali aquaculture, it is highly significant to explore the regulatory mechanisms of &...
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2025-01-01
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| author | Meiyao Wang Jun Zhou Jiachun Ge Gangchun Xu Yongkai Tang |
| author_facet | Meiyao Wang Jun Zhou Jiachun Ge Gangchun Xu Yongkai Tang |
| author_sort | Meiyao Wang |
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| description | (1) Background: Global climate change is intensifying, and the vigorous development and utilization of saline–alkali land is of great significance. As an important economic aquatic species in the context of saline–alkali aquaculture, it is highly significant to explore the regulatory mechanisms of <i>Eriocheir sinensis</i> under alkaline conditions. In particular, the brain (cerebral ganglion for crustaceans) serves as a vital regulatory organ in response to environmental stress; (2) Methods: In this study, a comparative transcriptome approach was employed to investigate the key regulatory genes and molecular regulatory mechanisms in the cerebral ganglion of <i>E. sinensis</i> under alkaline stress. (3) Results: The results demonstrated that the cerebral ganglion of <i>E. sinensis</i> exhibited a positive response to acute alkaline stress. Pathways associated with signal transduction and substance transportation, such as “phagosome” and “regulation of actin cytoskeleton”, along with regulatory genes involved in antioxidation, were upregulated synergistically to maintain homeostasis under alkaline stress. Furthermore, it was discovered for the first time that <i>bursicon</i> plays a positive regulatory role in the adaptation of <i>E. sinensis</i> to alkalinity. (4) Conclusions: The present study elucidates the molecular regulatory pattern of the cerebral ganglion in <i>E. sinensis</i> under acute alkaline stress as well as revealing a novel role of <i>bursicon</i> in facilitating adaptation to alkalinity in <i>E. sinensis</i>, providing valuable theoretical insights into the molecular regulatory mechanisms underlying the responses of cerebral ganglia to saline–alkali environments. These findings also offer a theoretical reference for promoting the sustainable development of the <i>E. sinensis</i> breeding industry under saline–alkali conditions. |
| format | Article |
| id | doaj-art-edd5ec8542044a238e01af24573e1dc0 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-edd5ec8542044a238e01af24573e1dc02025-01-24T13:23:34ZengMDPI AGBiology2079-77372025-01-011418410.3390/biology14010084Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline StressMeiyao Wang0Jun Zhou1Jiachun Ge2Gangchun Xu3Yongkai Tang4Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi 214081, ChinaFreshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaFreshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi 214081, China(1) Background: Global climate change is intensifying, and the vigorous development and utilization of saline–alkali land is of great significance. As an important economic aquatic species in the context of saline–alkali aquaculture, it is highly significant to explore the regulatory mechanisms of <i>Eriocheir sinensis</i> under alkaline conditions. In particular, the brain (cerebral ganglion for crustaceans) serves as a vital regulatory organ in response to environmental stress; (2) Methods: In this study, a comparative transcriptome approach was employed to investigate the key regulatory genes and molecular regulatory mechanisms in the cerebral ganglion of <i>E. sinensis</i> under alkaline stress. (3) Results: The results demonstrated that the cerebral ganglion of <i>E. sinensis</i> exhibited a positive response to acute alkaline stress. Pathways associated with signal transduction and substance transportation, such as “phagosome” and “regulation of actin cytoskeleton”, along with regulatory genes involved in antioxidation, were upregulated synergistically to maintain homeostasis under alkaline stress. Furthermore, it was discovered for the first time that <i>bursicon</i> plays a positive regulatory role in the adaptation of <i>E. sinensis</i> to alkalinity. (4) Conclusions: The present study elucidates the molecular regulatory pattern of the cerebral ganglion in <i>E. sinensis</i> under acute alkaline stress as well as revealing a novel role of <i>bursicon</i> in facilitating adaptation to alkalinity in <i>E. sinensis</i>, providing valuable theoretical insights into the molecular regulatory mechanisms underlying the responses of cerebral ganglia to saline–alkali environments. These findings also offer a theoretical reference for promoting the sustainable development of the <i>E. sinensis</i> breeding industry under saline–alkali conditions.https://www.mdpi.com/2079-7737/14/1/84<i>Eriocheir sinensis</i>cerebral gangliaalkalinity stress<i>bursicon</i>signal transduction |
| spellingShingle | Meiyao Wang Jun Zhou Jiachun Ge Gangchun Xu Yongkai Tang Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline Stress Biology <i>Eriocheir sinensis</i> cerebral ganglia alkalinity stress <i>bursicon</i> signal transduction |
| title | Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline Stress |
| title_full | Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline Stress |
| title_fullStr | Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline Stress |
| title_full_unstemmed | Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline Stress |
| title_short | Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via <i>Bursicon</i> in the Cerebral Ganglion of Chinese Mitten Crab <i>Eriocheir sinensis</i> Under Alkaline Stress |
| title_sort | molecular mechanisms underlying substance transport signal transduction and anti stress regulation as well as anti alkaline regulation via i bursicon i in the cerebral ganglion of chinese mitten crab i eriocheir sinensis i under alkaline stress |
| topic | <i>Eriocheir sinensis</i> cerebral ganglia alkalinity stress <i>bursicon</i> signal transduction |
| url | https://www.mdpi.com/2079-7737/14/1/84 |
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