Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic Mice
Introduction: An exercise-induced myokine, Irisin, is shown to cause the browning of white adipose tissue, promote glycogen storage, and inhibit glucose and cholesterol formation in the liver. Studies have, however, shown a reduction of circulating irisin in type 2 diabetes, suggesting altered secr...
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Shaikh Zayed Medical Complex, Lahore
2025-03-01
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| Series: | Proceedings |
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| Online Access: | https://mail.proceedings-szmc.org.pk/index.php/szmc/article/view/476 |
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| author | Sana Akram Hira Zahid Maimona Tabbsum Zehra Niazi Jahanzab Salim Chaman Nasrullah |
| author_facet | Sana Akram Hira Zahid Maimona Tabbsum Zehra Niazi Jahanzab Salim Chaman Nasrullah |
| author_sort | Sana Akram |
| collection | DOAJ |
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Introduction: An exercise-induced myokine, Irisin, is shown to cause the browning of white adipose tissue, promote glycogen storage, and inhibit glucose and cholesterol formation in the liver. Studies have, however, shown a reduction of circulating irisin in type 2 diabetes, suggesting altered secretion of myokines in the setting of insulin resistance.
Aims & Objectives: This study explored the effect of recombinant irisin on insulin resistance and oxidative strain in laboratory induced type 2 diabetic mice.
Place and Duration of Study: Animal House and Laboratory of Akhter Saeed Medical and Dental College, Lahore from March 2018 to December 2018.
Material & Methods: A randomized comparative trial was conducted. Type 2 diabetes mellitus was produced in 60 male albino mice by feeding them a fat rich diet (60% kcal fat) for 6 weeks, followed by low dose streptozotocin (40 mg/kg body weight). Thirty diabetic mice acted as diabetic controls and thirty mice were administered recombinant irisin intra-peritoneally, 1mg/kg body weight daily for 4 weeks. Serum fasting glucose, insulin, lipid profile, and malondialdehyde were estimated thereafter. Insulin resistance was predicted by employing the HOMA-IR score and triglyceride-HDL ratio. The data was analyzed by using SPSS (version 21), a p value less than 0.05 was regarded statistically significant
Results: Diabetic mice that received recombinant irisin showed significant (p=0.000) reduction in fasting blood glucose (249.03±66.17mg/dl) and serum insulin (4.61±0.08µU/L) as compared to the diabetic control mice (428.50±78.13 mg/dl, 5.95±0.72µU/L). The degree of insulin sensitivity as determined by HOMA-IR score and triglyceride HDL ratio was significantly increased in the diabetic irisin groups as compared to the diabetic control group. This correlated with reduction in the oxidative stress marker malondialdehyde.
Conclusion: Recombinant Irisin improved insulin sensitivity and reduced oxidative strain in type 2 diabetic mice.
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| format | Article |
| id | doaj-art-edb539123e3a43d8bf6b86a7f6ba51e1 |
| institution | OA Journals |
| issn | 1815-4905 2518-203X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Shaikh Zayed Medical Complex, Lahore |
| record_format | Article |
| series | Proceedings |
| spelling | doaj-art-edb539123e3a43d8bf6b86a7f6ba51e12025-08-20T02:36:09ZengShaikh Zayed Medical Complex, LahoreProceedings1815-49052518-203X2025-03-0139110.47489/szmc.v39i1.476Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic MiceSana Akram0Hira Zahid1Maimona Tabbsum2Zehra Niazi3Jahanzab Salim4Chaman Nasrullah5University Medical & Dental College, The University of FaisalabadUniversity Medical & Dental College, The University of FaisalabadAkhter Saeed Medical & Dental College, LahoreUniversity Medical & Dental College, The University of FaisalabadKhawaja Muhammad Safdar Medical College, SialkotUniversity College of Medicine & Dentistry, The University of Lahore Introduction: An exercise-induced myokine, Irisin, is shown to cause the browning of white adipose tissue, promote glycogen storage, and inhibit glucose and cholesterol formation in the liver. Studies have, however, shown a reduction of circulating irisin in type 2 diabetes, suggesting altered secretion of myokines in the setting of insulin resistance. Aims & Objectives: This study explored the effect of recombinant irisin on insulin resistance and oxidative strain in laboratory induced type 2 diabetic mice. Place and Duration of Study: Animal House and Laboratory of Akhter Saeed Medical and Dental College, Lahore from March 2018 to December 2018. Material & Methods: A randomized comparative trial was conducted. Type 2 diabetes mellitus was produced in 60 male albino mice by feeding them a fat rich diet (60% kcal fat) for 6 weeks, followed by low dose streptozotocin (40 mg/kg body weight). Thirty diabetic mice acted as diabetic controls and thirty mice were administered recombinant irisin intra-peritoneally, 1mg/kg body weight daily for 4 weeks. Serum fasting glucose, insulin, lipid profile, and malondialdehyde were estimated thereafter. Insulin resistance was predicted by employing the HOMA-IR score and triglyceride-HDL ratio. The data was analyzed by using SPSS (version 21), a p value less than 0.05 was regarded statistically significant Results: Diabetic mice that received recombinant irisin showed significant (p=0.000) reduction in fasting blood glucose (249.03±66.17mg/dl) and serum insulin (4.61±0.08µU/L) as compared to the diabetic control mice (428.50±78.13 mg/dl, 5.95±0.72µU/L). The degree of insulin sensitivity as determined by HOMA-IR score and triglyceride HDL ratio was significantly increased in the diabetic irisin groups as compared to the diabetic control group. This correlated with reduction in the oxidative stress marker malondialdehyde. Conclusion: Recombinant Irisin improved insulin sensitivity and reduced oxidative strain in type 2 diabetic mice. https://mail.proceedings-szmc.org.pk/index.php/szmc/article/view/476Irisininsulin resistanceoxidative stressdiabetes mellitus type 2 |
| spellingShingle | Sana Akram Hira Zahid Maimona Tabbsum Zehra Niazi Jahanzab Salim Chaman Nasrullah Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic Mice Proceedings Irisin insulin resistance oxidative stress diabetes mellitus type 2 |
| title | Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic Mice |
| title_full | Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic Mice |
| title_fullStr | Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic Mice |
| title_full_unstemmed | Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic Mice |
| title_short | Recombinant Irisin Ameliorates Insulin Resistance in Type 2 Diabetic Mice |
| title_sort | recombinant irisin ameliorates insulin resistance in type 2 diabetic mice |
| topic | Irisin insulin resistance oxidative stress diabetes mellitus type 2 |
| url | https://mail.proceedings-szmc.org.pk/index.php/szmc/article/view/476 |
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