Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailing

Microplastics (MPs) are present throughout the environment, and due to their nature, they are extremely difficult to decompose. Reportedly, microorganisms play an important role in degrading and decomposing MPs. Bacillus pasteurii can degrade various complex organic matter, including MPs, which are...

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Main Authors: Zhichao Wang, Yaxinyue Li, Long Bai, Chenli Hou, Chunli Zheng, Weiping Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-03-01
Series:Emerging Contaminants
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405665024000982
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author Zhichao Wang
Yaxinyue Li
Long Bai
Chenli Hou
Chunli Zheng
Weiping Li
author_facet Zhichao Wang
Yaxinyue Li
Long Bai
Chenli Hou
Chunli Zheng
Weiping Li
author_sort Zhichao Wang
collection DOAJ
description Microplastics (MPs) are present throughout the environment, and due to their nature, they are extremely difficult to decompose. Reportedly, microorganisms play an important role in degrading and decomposing MPs. Bacillus pasteurii can degrade various complex organic matter, including MPs, which are a class of polymeric organic compounds. This study investigated the degradation effect of B. pasteurii on polypropylene MPs (PP-MPs) in soil. B. pasteurii was extracted from gold mine tailings. Herein, three experimental groups were established—a blank control treatment group, a group with bacteria without Ca2+ added (T2 group), and a group with bacteria supplemented with Ca2+ (T3 group)—for a 30-day indoor simulation of MP degradation in MP-treated soil. The results showed that the total mass change rate of the PP-MPs in the T2 group was 20.95 %, and grooves and holes appeared on the PP-MP surfaces. The total mass change rate of the PP-MPs in the T3 group was 23.22 %, and abundant fissures and pits appeared on the PP-MP surfaces. Additionally, new dominant phyla, such as Bacteroidetes and Firmicutes, appeared after bacterial addition. The relative abundance of several common soil genera, such as Bacillus, Brevundimonas, Flavobacterium, and Arthrobacter, and genera capable of breaking down complex compounds increased after B. pasteurii addition. The soil microbial community diversity improved, with the distribution of each species being relatively uniform. These findings indicated that the B. pasteurii strain can be used to degrade PP-MPs. Additionally, the addition of Ca2+ generated microbially induced calcium carbonate precipitation, which further improved the degradation of MPs. This study provides theoretical support for studying the degradation mechanism of PP-MPs.
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spelling doaj-art-edac1946b8d14d0b8d13f8564ba208602025-08-20T02:27:47ZengKeAi Communications Co., Ltd.Emerging Contaminants2405-66502025-03-0111110039710.1016/j.emcon.2024.100397Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailingZhichao Wang0Yaxinyue Li1Long Bai2Chenli Hou3Chunli Zheng4Weiping Li5College of Environment and Energy Resources, Inner Mongolia University of Science and Technology, Baotou, 014010, ChinaCollege of Environment and Energy Resources, Inner Mongolia University of Science and Technology, Baotou, 014010, ChinaCollege of Environment and Energy Resources, Inner Mongolia University of Science and Technology, Baotou, 014010, ChinaCollege of Environment and Energy Resources, Inner Mongolia University of Science and Technology, Baotou, 014010, ChinaCollege of Faculty of Resources and Environmental Engineering, Shanghai Polytechnic University, Shanghai, 201209, China; Corresponding author.College of Environment and Energy Resources, Inner Mongolia University of Science and Technology, Baotou, 014010, China; Corresponding author. College of Environment and Energy Resources, Inner Mongolia University of Science and Technology, No. 7, Alding Street, Kundulun District, Baotou, 014010, China.Microplastics (MPs) are present throughout the environment, and due to their nature, they are extremely difficult to decompose. Reportedly, microorganisms play an important role in degrading and decomposing MPs. Bacillus pasteurii can degrade various complex organic matter, including MPs, which are a class of polymeric organic compounds. This study investigated the degradation effect of B. pasteurii on polypropylene MPs (PP-MPs) in soil. B. pasteurii was extracted from gold mine tailings. Herein, three experimental groups were established—a blank control treatment group, a group with bacteria without Ca2+ added (T2 group), and a group with bacteria supplemented with Ca2+ (T3 group)—for a 30-day indoor simulation of MP degradation in MP-treated soil. The results showed that the total mass change rate of the PP-MPs in the T2 group was 20.95 %, and grooves and holes appeared on the PP-MP surfaces. The total mass change rate of the PP-MPs in the T3 group was 23.22 %, and abundant fissures and pits appeared on the PP-MP surfaces. Additionally, new dominant phyla, such as Bacteroidetes and Firmicutes, appeared after bacterial addition. The relative abundance of several common soil genera, such as Bacillus, Brevundimonas, Flavobacterium, and Arthrobacter, and genera capable of breaking down complex compounds increased after B. pasteurii addition. The soil microbial community diversity improved, with the distribution of each species being relatively uniform. These findings indicated that the B. pasteurii strain can be used to degrade PP-MPs. Additionally, the addition of Ca2+ generated microbially induced calcium carbonate precipitation, which further improved the degradation of MPs. This study provides theoretical support for studying the degradation mechanism of PP-MPs.http://www.sciencedirect.com/science/article/pii/S2405665024000982Plastic pollutionPolypropyleneBiodegradationBacillus pasteuriiMICPSoil
spellingShingle Zhichao Wang
Yaxinyue Li
Long Bai
Chenli Hou
Chunli Zheng
Weiping Li
Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailing
Emerging Contaminants
Plastic pollution
Polypropylene
Biodegradation
Bacillus pasteurii
MICP
Soil
title Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailing
title_full Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailing
title_fullStr Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailing
title_full_unstemmed Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailing
title_short Biodegradation of polypropylene microplastics by Bacillus pasteurii isolated from a gold mine tailing
title_sort biodegradation of polypropylene microplastics by bacillus pasteurii isolated from a gold mine tailing
topic Plastic pollution
Polypropylene
Biodegradation
Bacillus pasteurii
MICP
Soil
url http://www.sciencedirect.com/science/article/pii/S2405665024000982
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