Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitors
The effects of PDE inhibitors on oxazolone-induced contact hypersensitivity (CS) were studied in mice. Rolipram, Ro 20-1724 and theophylline dose dependently inhibited CS but none caused >53% inhibition. ED30 values at 24 h before challenge for rolipram, Ro 20-1724 and theophylline were 2.1, 5.4...
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000196 |
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| author | I. Moodley Y. Sotsios B. Bertin |
| author_facet | I. Moodley Y. Sotsios B. Bertin |
| author_sort | I. Moodley |
| collection | DOAJ |
| description | The effects of PDE inhibitors on oxazolone-induced contact hypersensitivity (CS) were studied in mice. Rolipram, Ro 20-1724 and theophylline dose dependently inhibited CS but none caused >53% inhibition. ED30 values at 24 h before challenge for rolipram, Ro 20-1724 and theophylline were 2.1, 5.4 and 30.4 mg/kg, p.o., respectively. Milrinone and SKF 94836 at 30 mg/kg caused a small, but significant inhibition of 13% and 18%, respectively, although the inhibition (8%) caused by zaprinast was not significant. Betamethasone (10 mg/kg, p.o.) caused a marked inhibition (80%) as did indomethacin (65% at 5 mg/kg, p.o.). Rolipram and Ro 20-1724 inhibited proliferation of mouse lymphoblasts with IC50 values of 0.08 μM and 0.83 μM, respectively. In contrast, zaprinast caused only a weak inhibition (IC50 = 119 μM) of lymphocyte proliferation, whereas SKF 94836 and theophylline failed to cause any significant inhibition at 100 μM (26% and 2%, respectively). These findings suggest that PDE IV isozymes play a principal role in mediating CS by inhibiting lymphocyte activation. |
| format | Article |
| id | doaj-art-ed85853495b34f46982e799984cd5f9b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ed85853495b34f46982e799984cd5f9b2025-02-03T06:14:01ZengWileyMediators of Inflammation0962-93511466-18611995-01-014211211610.1155/S0962935195000196Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitorsI. Moodley0Y. Sotsios1B. Bertin2Institut de Recherche Jouveinal, 9 rue de la Loge, Fresnes Cedex 94265, FranceInstitut de Recherche Jouveinal, 9 rue de la Loge, Fresnes Cedex 94265, FranceInstitut de Recherche Jouveinal, 9 rue de la Loge, Fresnes Cedex 94265, FranceThe effects of PDE inhibitors on oxazolone-induced contact hypersensitivity (CS) were studied in mice. Rolipram, Ro 20-1724 and theophylline dose dependently inhibited CS but none caused >53% inhibition. ED30 values at 24 h before challenge for rolipram, Ro 20-1724 and theophylline were 2.1, 5.4 and 30.4 mg/kg, p.o., respectively. Milrinone and SKF 94836 at 30 mg/kg caused a small, but significant inhibition of 13% and 18%, respectively, although the inhibition (8%) caused by zaprinast was not significant. Betamethasone (10 mg/kg, p.o.) caused a marked inhibition (80%) as did indomethacin (65% at 5 mg/kg, p.o.). Rolipram and Ro 20-1724 inhibited proliferation of mouse lymphoblasts with IC50 values of 0.08 μM and 0.83 μM, respectively. In contrast, zaprinast caused only a weak inhibition (IC50 = 119 μM) of lymphocyte proliferation, whereas SKF 94836 and theophylline failed to cause any significant inhibition at 100 μM (26% and 2%, respectively). These findings suggest that PDE IV isozymes play a principal role in mediating CS by inhibiting lymphocyte activation.http://dx.doi.org/10.1155/S0962935195000196 |
| spellingShingle | I. Moodley Y. Sotsios B. Bertin Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitors Mediators of Inflammation |
| title | Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitors |
| title_full | Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitors |
| title_fullStr | Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitors |
| title_full_unstemmed | Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitors |
| title_short | Modulation of oxazolone-induced hypersensitivity in mice by selective PDE inhibitors |
| title_sort | modulation of oxazolone induced hypersensitivity in mice by selective pde inhibitors |
| url | http://dx.doi.org/10.1155/S0962935195000196 |
| work_keys_str_mv | AT imoodley modulationofoxazoloneinducedhypersensitivityinmicebyselectivepdeinhibitors AT ysotsios modulationofoxazoloneinducedhypersensitivityinmicebyselectivepdeinhibitors AT bbertin modulationofoxazoloneinducedhypersensitivityinmicebyselectivepdeinhibitors |