Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC Method
ABSTRACT Background The matrix effect is accepted as one of the critical factors that affect trueness, and it is especially important in the analysis of critical compounds, such as drugs, toxins and pesticides, in complex matrices. Ceftiofur (CEF) is a leading drug used in dairy industry for pulmona...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70080 |
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| author | Saniye Özcan Serkan Levent Nafiz Öncü Can |
| author_facet | Saniye Özcan Serkan Levent Nafiz Öncü Can |
| author_sort | Saniye Özcan |
| collection | DOAJ |
| description | ABSTRACT Background The matrix effect is accepted as one of the critical factors that affect trueness, and it is especially important in the analysis of critical compounds, such as drugs, toxins and pesticides, in complex matrices. Ceftiofur (CEF) is a leading drug used in dairy industry for pulmonary infections. Because milk has a complex matrix, which is rich in fats, proteins and many other compounds, the determination of CEF in milk products deals with issues of trueness. On the other hand, pharmaceuticals also have a complex composition as they contain not only the active pharmaceutical ingredient but also many excipients, such as preservatives and pH modifiers. Due to its frequent use, its residues can be found in meat products or exist as an excreted compound in urine, milk and so on. Objective The aim of this study was to investigate the effect of different calibration methods on quantitative estimation of CEF in milk and pharmaceutical samples. Methods CEF was analysed using a new HPLC method, in which separation was achieved on a C18 bonded fused‐core silica particle column by using isocratic elution; the method was optimized by testing different chromatographic conditions and validated according to the ICH Q2(R1) and United States Pharmacopeia guidelines. Results Interestingly, it was found that CEF signals in milk samples were higher than the ones at the same level prepared in calibration solutions with a ratio of 11.28:1. Conclusion This finding reveals the necessity of matrix‐matched calibration for accurate quantitative determination of CEF in milk samples. Moreover, the milk in the market and the most used pharmaceutical formulations were analysed using the current method. |
| format | Article |
| id | doaj-art-ed7e0c4cc9594881ab337df474e59ac4 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-ed7e0c4cc9594881ab337df474e59ac42025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70080Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC MethodSaniye Özcan0Serkan Levent1Nafiz Öncü Can2Department of Analytical Chemistry, Faculty of Pharmacy Anadolu University Eskisehir TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy Anadolu University Eskisehir TurkeyDepartment of Analytical Chemistry, Faculty of Pharmacy Anadolu University Eskisehir TurkeyABSTRACT Background The matrix effect is accepted as one of the critical factors that affect trueness, and it is especially important in the analysis of critical compounds, such as drugs, toxins and pesticides, in complex matrices. Ceftiofur (CEF) is a leading drug used in dairy industry for pulmonary infections. Because milk has a complex matrix, which is rich in fats, proteins and many other compounds, the determination of CEF in milk products deals with issues of trueness. On the other hand, pharmaceuticals also have a complex composition as they contain not only the active pharmaceutical ingredient but also many excipients, such as preservatives and pH modifiers. Due to its frequent use, its residues can be found in meat products or exist as an excreted compound in urine, milk and so on. Objective The aim of this study was to investigate the effect of different calibration methods on quantitative estimation of CEF in milk and pharmaceutical samples. Methods CEF was analysed using a new HPLC method, in which separation was achieved on a C18 bonded fused‐core silica particle column by using isocratic elution; the method was optimized by testing different chromatographic conditions and validated according to the ICH Q2(R1) and United States Pharmacopeia guidelines. Results Interestingly, it was found that CEF signals in milk samples were higher than the ones at the same level prepared in calibration solutions with a ratio of 11.28:1. Conclusion This finding reveals the necessity of matrix‐matched calibration for accurate quantitative determination of CEF in milk samples. Moreover, the milk in the market and the most used pharmaceutical formulations were analysed using the current method.https://doi.org/10.1002/vms3.70080ceftiofurHigh Performance Liquid Chromatography (HPLC)matrix effect matrix‐matched calibrationmilkpharmaceutical preparation |
| spellingShingle | Saniye Özcan Serkan Levent Nafiz Öncü Can Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC Method Veterinary Medicine and Science ceftiofur High Performance Liquid Chromatography (HPLC) matrix effect matrix‐matched calibration milk pharmaceutical preparation |
| title | Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC Method |
| title_full | Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC Method |
| title_fullStr | Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC Method |
| title_full_unstemmed | Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC Method |
| title_short | Solvent‐Based and Matrix‐Matched Calibration Methods on Analysis of Ceftiofur Residues in Milk and Pharmaceutical Samples Using a Novel HPLC Method |
| title_sort | solvent based and matrix matched calibration methods on analysis of ceftiofur residues in milk and pharmaceutical samples using a novel hplc method |
| topic | ceftiofur High Performance Liquid Chromatography (HPLC) matrix effect matrix‐matched calibration milk pharmaceutical preparation |
| url | https://doi.org/10.1002/vms3.70080 |
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