A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid
Viral meningitis poses a significant clinical challenge due to its rapid onset and potential progression to life-threatening encephalitis. Early detection of treatable viral pathogens such as Herpes simplex virus (HSV), Cytomegalovirus (CMV), and Varicella-zoster virus (VZV) is essential for initiat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/1/111 |
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| author | Tatjana Luzius Samuel D. Jeske Julia Baer Uta Goelnitz Ulrike Protzer Jochen M. Wettengel |
| author_facet | Tatjana Luzius Samuel D. Jeske Julia Baer Uta Goelnitz Ulrike Protzer Jochen M. Wettengel |
| author_sort | Tatjana Luzius |
| collection | DOAJ |
| description | Viral meningitis poses a significant clinical challenge due to its rapid onset and potential progression to life-threatening encephalitis. Early detection of treatable viral pathogens such as Herpes simplex virus (HSV), Cytomegalovirus (CMV), and Varicella-zoster virus (VZV) is essential for initiating appropriate therapies. However, multiplex PCRs for the rapid and simultaneous detection of these pathogens are scarce due to the complex PCR design and the elaborate validation process using cerebrospinal fluid samples. In this study, we established and validated a novel multiplex PCR assay for detecting HSV, CMV, and VZV in cerebrospinal fluid samples and implemented the assay on a fully automated platform. |
| format | Article |
| id | doaj-art-ed752a046d774daab5d9210c5ae56ad7 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-ed752a046d774daab5d9210c5ae56ad72025-01-24T13:42:41ZengMDPI AGMicroorganisms2076-26072025-01-0113111110.3390/microorganisms13010111A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal FluidTatjana Luzius0Samuel D. Jeske1Julia Baer2Uta Goelnitz3Ulrike Protzer4Jochen M. Wettengel5School of Medicine and Health, Institute of Virology, Technical University of Munich/Helmholtz Munich, 81675 Munich, GermanySchool of Medicine and Health, Institute of Virology, Technical University of Munich/Helmholtz Munich, 81675 Munich, GermanyQIAGEN GmbH, Strategic Lab Consultancy, 40724 Hilden, GermanyQIAGEN GmbH, Strategic Lab Consultancy, 40724 Hilden, GermanySchool of Medicine and Health, Institute of Virology, Technical University of Munich/Helmholtz Munich, 81675 Munich, GermanySchool of Medicine and Health, Institute of Virology, Technical University of Munich/Helmholtz Munich, 81675 Munich, GermanyViral meningitis poses a significant clinical challenge due to its rapid onset and potential progression to life-threatening encephalitis. Early detection of treatable viral pathogens such as Herpes simplex virus (HSV), Cytomegalovirus (CMV), and Varicella-zoster virus (VZV) is essential for initiating appropriate therapies. However, multiplex PCRs for the rapid and simultaneous detection of these pathogens are scarce due to the complex PCR design and the elaborate validation process using cerebrospinal fluid samples. In this study, we established and validated a novel multiplex PCR assay for detecting HSV, CMV, and VZV in cerebrospinal fluid samples and implemented the assay on a fully automated platform.https://www.mdpi.com/2076-2607/13/1/111multiplex PCRviral meningitisclinical virology |
| spellingShingle | Tatjana Luzius Samuel D. Jeske Julia Baer Uta Goelnitz Ulrike Protzer Jochen M. Wettengel A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid Microorganisms multiplex PCR viral meningitis clinical virology |
| title | A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid |
| title_full | A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid |
| title_fullStr | A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid |
| title_full_unstemmed | A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid |
| title_short | A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid |
| title_sort | multiplex polymerase chain reaction assay for the detection of herpes simplex virus cytomegalovirus and varicella zoster virus in cerebrospinal fluid |
| topic | multiplex PCR viral meningitis clinical virology |
| url | https://www.mdpi.com/2076-2607/13/1/111 |
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