Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines
The aim of this study was to assess the biological effect of organically coated Grias neuberthii (piton) fruit and Persea americana (avocado) leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2018/9689131 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556418313486336 |
|---|---|
| author | Lizeth Salazar María José Vallejo López Marcelo Grijalva Luis Castillo Alexander Maldonado |
| author_facet | Lizeth Salazar María José Vallejo López Marcelo Grijalva Luis Castillo Alexander Maldonado |
| author_sort | Lizeth Salazar |
| collection | DOAJ |
| description | The aim of this study was to assess the biological effect of organically coated Grias neuberthii (piton) fruit and Persea americana (avocado) leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and glutathione-S-transferase GST) and cell viability. UV-Vis spectroscopy analysis showed that synthesized AgNPs remained partially stable under cell culture conditions. HeLa cells remained viable when exposed to piton and avocado AgNPs. A statistically significant, dose-dependent cytotoxic response to both AgNPs was found on the breast cancer (MCF-7) cell line at concentrations above 50 µM. While expression levels of transcription factor p53 showed downregulation in treated MCF-7 and HeLa cells, GST expression was not affected in both cell lines treated. Cell viability assays along with gene expression levels in treated MCF-7 cells support a cancer cell population undergoing cell cycle arrest. The selective toxicity of biosynthesized piton/avocado AgNPs on MCF-7 cells might be of value for novel therapeutics. |
| format | Article |
| id | doaj-art-ed5e32dbeaae466d803bd8b0b0294c68 |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-ed5e32dbeaae466d803bd8b0b0294c682025-02-03T05:45:31ZengWileyJournal of Nanotechnology1687-95031687-95112018-01-01201810.1155/2018/96891319689131Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell LinesLizeth Salazar0María José Vallejo López1Marcelo Grijalva2Luis Castillo3Alexander Maldonado4Centro de Nanociencia y Nanotecnología, Universidad de las Fuerzas Armadas ESPE, Avenida General Rumiñahui S/N y Ambato, P.O. Box 171-5-231B, Sangolquí, EcuadorDepartamento de Ciencias de la Vida, Universidad de las Fuerzas Armadas ESPE, Avenida General Rumiñahui S/N y Ambato, P.O. Box 171-5-231B, Sangolquí, EcuadorCentro de Nanociencia y Nanotecnología, Universidad de las Fuerzas Armadas ESPE, Avenida General Rumiñahui S/N y Ambato, P.O. Box 171-5-231B, Sangolquí, EcuadorFacultad de Ingeniería, Ciencias Físicas y Matemáticas, Universidad Central del Ecuador, P.O. Box 1701521, Quito, EcuadorCentro de Nanociencia y Nanotecnología, Universidad de las Fuerzas Armadas ESPE, Avenida General Rumiñahui S/N y Ambato, P.O. Box 171-5-231B, Sangolquí, EcuadorThe aim of this study was to assess the biological effect of organically coated Grias neuberthii (piton) fruit and Persea americana (avocado) leaves nanoparticles (NPs) on cervical cancer (HeLa) and breast adenocarcinoma (MCF-7) cells with an emphasis on gene expression (p53 transcription factor and glutathione-S-transferase GST) and cell viability. UV-Vis spectroscopy analysis showed that synthesized AgNPs remained partially stable under cell culture conditions. HeLa cells remained viable when exposed to piton and avocado AgNPs. A statistically significant, dose-dependent cytotoxic response to both AgNPs was found on the breast cancer (MCF-7) cell line at concentrations above 50 µM. While expression levels of transcription factor p53 showed downregulation in treated MCF-7 and HeLa cells, GST expression was not affected in both cell lines treated. Cell viability assays along with gene expression levels in treated MCF-7 cells support a cancer cell population undergoing cell cycle arrest. The selective toxicity of biosynthesized piton/avocado AgNPs on MCF-7 cells might be of value for novel therapeutics.http://dx.doi.org/10.1155/2018/9689131 |
| spellingShingle | Lizeth Salazar María José Vallejo López Marcelo Grijalva Luis Castillo Alexander Maldonado Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines Journal of Nanotechnology |
| title | Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines |
| title_full | Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines |
| title_fullStr | Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines |
| title_full_unstemmed | Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines |
| title_short | Biological Effect of Organically Coated Grias neuberthii and Persea americana Silver Nanoparticles on HeLa and MCF-7 Cancer Cell Lines |
| title_sort | biological effect of organically coated grias neuberthii and persea americana silver nanoparticles on hela and mcf 7 cancer cell lines |
| url | http://dx.doi.org/10.1155/2018/9689131 |
| work_keys_str_mv | AT lizethsalazar biologicaleffectoforganicallycoatedgriasneuberthiiandperseaamericanasilvernanoparticlesonhelaandmcf7cancercelllines AT mariajosevallejolopez biologicaleffectoforganicallycoatedgriasneuberthiiandperseaamericanasilvernanoparticlesonhelaandmcf7cancercelllines AT marcelogrijalva biologicaleffectoforganicallycoatedgriasneuberthiiandperseaamericanasilvernanoparticlesonhelaandmcf7cancercelllines AT luiscastillo biologicaleffectoforganicallycoatedgriasneuberthiiandperseaamericanasilvernanoparticlesonhelaandmcf7cancercelllines AT alexandermaldonado biologicaleffectoforganicallycoatedgriasneuberthiiandperseaamericanasilvernanoparticlesonhelaandmcf7cancercelllines |