Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp.
Foodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. In...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2019/8942608 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556418080702464 |
|---|---|
| author | Sylvain Daton Kougblénou Alidéhou Jerrold Agbankpé Justin Gbèssohélé Béhanzin Tamègnon Victorien Dougnon Alidah Victonie Aniambossou Lamine Baba-Moussa Honoré Sourou Bankolé |
| author_facet | Sylvain Daton Kougblénou Alidéhou Jerrold Agbankpé Justin Gbèssohélé Béhanzin Tamègnon Victorien Dougnon Alidah Victonie Aniambossou Lamine Baba-Moussa Honoré Sourou Bankolé |
| author_sort | Sylvain Daton Kougblénou |
| collection | DOAJ |
| description | Foodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. In Southern Benin, an excessive use of poultry manure as fertilizer in vegetable farms was noted. This survey aimed to determine the prevalence and concentration of Campylobacter spp., especially Campylobacter jejuni and Campylobacter coli, in selected environmental samples (poultry manure, and irrigation water) and freshly harvested leafy vegetables in two (Houeyiho and Sèmè-Kpodji) vegetable farms in southern Benin. To achieve this objective, we analyzed 280 samples, including 224 samples of leafy vegetables (Solanum macrocarpon and Lactuca sativa capita), 28 samples of irrigation water, and 28 samples of poultry manure. The analysis of the samples taken was carried out according to the modified NF EN ISO 10272-1 standard. Of the 280 samples analyzed in this survey, 63 were positive for Campylobacter contamination. For leafy vegetable samples analyzed in this survey, the contamination rate was of 15.63%. 60.71% of poultry manure samples analyzed were contaminated with Campylobacter spp. and 39.29% of irrigation water samples were contaminated. The statistical analysis of these results showed that there is a correlation between the contamination of leafy vegetables, poultry manure, and irrigations (p<0.01). Campylobacter jejuni (53.97%) was more involved in contaminations than Campylobacter coli (36.57%). This study has shown that there is a real risk of food poisoning by Campylobacter jejuni and Campylobacter coli among consumers of leafy vegetables in southern Benin. The origin of contamination of these leafy vegetables is poultry manure used as fertilizer in vegetable gardens and irrigation water used. |
| format | Article |
| id | doaj-art-ed5b1b45f05e4745a30a63e056b9ba94 |
| institution | Kabale University |
| issn | 2356-7015 2314-5765 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-ed5b1b45f05e4745a30a63e056b9ba942025-02-03T05:45:28ZengWileyInternational Journal of Food Science2356-70152314-57652019-01-01201910.1155/2019/89426088942608Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp.Sylvain Daton Kougblénou0Alidéhou Jerrold Agbankpé1Justin Gbèssohélé Béhanzin2Tamègnon Victorien Dougnon3Alidah Victonie Aniambossou4Lamine Baba-Moussa5Honoré Sourou Bankolé6Laboratory of Food Microbiology, Ministry of Health, 01 P.O. Box 418, Cotonou, BeninResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, BeninLaboratory of Molecular Physiopathology and Toxicology, Faculty of Science and Technology, University of Abomey-Calavi, 01 P.O. Box 4521, Cotonou, BeninResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, BeninResearch Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, BeninLaboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, 05 P.O. Box 1604, Cotonou, BeninLaboratory of Food Microbiology, Ministry of Health, 01 P.O. Box 418, Cotonou, BeninFoodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. In Southern Benin, an excessive use of poultry manure as fertilizer in vegetable farms was noted. This survey aimed to determine the prevalence and concentration of Campylobacter spp., especially Campylobacter jejuni and Campylobacter coli, in selected environmental samples (poultry manure, and irrigation water) and freshly harvested leafy vegetables in two (Houeyiho and Sèmè-Kpodji) vegetable farms in southern Benin. To achieve this objective, we analyzed 280 samples, including 224 samples of leafy vegetables (Solanum macrocarpon and Lactuca sativa capita), 28 samples of irrigation water, and 28 samples of poultry manure. The analysis of the samples taken was carried out according to the modified NF EN ISO 10272-1 standard. Of the 280 samples analyzed in this survey, 63 were positive for Campylobacter contamination. For leafy vegetable samples analyzed in this survey, the contamination rate was of 15.63%. 60.71% of poultry manure samples analyzed were contaminated with Campylobacter spp. and 39.29% of irrigation water samples were contaminated. The statistical analysis of these results showed that there is a correlation between the contamination of leafy vegetables, poultry manure, and irrigations (p<0.01). Campylobacter jejuni (53.97%) was more involved in contaminations than Campylobacter coli (36.57%). This study has shown that there is a real risk of food poisoning by Campylobacter jejuni and Campylobacter coli among consumers of leafy vegetables in southern Benin. The origin of contamination of these leafy vegetables is poultry manure used as fertilizer in vegetable gardens and irrigation water used.http://dx.doi.org/10.1155/2019/8942608 |
| spellingShingle | Sylvain Daton Kougblénou Alidéhou Jerrold Agbankpé Justin Gbèssohélé Béhanzin Tamègnon Victorien Dougnon Alidah Victonie Aniambossou Lamine Baba-Moussa Honoré Sourou Bankolé Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp. International Journal of Food Science |
| title | Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp. |
| title_full | Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp. |
| title_fullStr | Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp. |
| title_full_unstemmed | Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp. |
| title_short | Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp. |
| title_sort | microbiological safety of leafy vegetables produced at houeyiho and seme kpodji vegetable farms in southern benin risk factors for campylobacter spp |
| url | http://dx.doi.org/10.1155/2019/8942608 |
| work_keys_str_mv | AT sylvaindatonkougblenou microbiologicalsafetyofleafyvegetablesproducedathoueyihoandsemekpodjivegetablefarmsinsouthernbeninriskfactorsforcampylobacterspp AT alidehoujerroldagbankpe microbiologicalsafetyofleafyvegetablesproducedathoueyihoandsemekpodjivegetablefarmsinsouthernbeninriskfactorsforcampylobacterspp AT justingbessohelebehanzin microbiologicalsafetyofleafyvegetablesproducedathoueyihoandsemekpodjivegetablefarmsinsouthernbeninriskfactorsforcampylobacterspp AT tamegnonvictoriendougnon microbiologicalsafetyofleafyvegetablesproducedathoueyihoandsemekpodjivegetablefarmsinsouthernbeninriskfactorsforcampylobacterspp AT alidahvictonieaniambossou microbiologicalsafetyofleafyvegetablesproducedathoueyihoandsemekpodjivegetablefarmsinsouthernbeninriskfactorsforcampylobacterspp AT laminebabamoussa microbiologicalsafetyofleafyvegetablesproducedathoueyihoandsemekpodjivegetablefarmsinsouthernbeninriskfactorsforcampylobacterspp AT honoresouroubankole microbiologicalsafetyofleafyvegetablesproducedathoueyihoandsemekpodjivegetablefarmsinsouthernbeninriskfactorsforcampylobacterspp |