The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model
<b>Objective:</b> To evaluate the drug release, cytocompatibility with periodontal ligament cells (PDLCs), and therapeutic efficacy of GelMA hydrogel loaded with resolvin D1 (RvD1) in treating rat periodontal inflammation and alveolar bone damage. <b>Methods:</b> An RvD1 comp...
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| author | Zhe Xing Jing Liu Jiazheng Cai Xiaofeng Jiang Jingwen Liang Masahito Fujio Elin Hadler-Olsen Jing Wang Alpdogan Kantarci Ying Xue |
| author_facet | Zhe Xing Jing Liu Jiazheng Cai Xiaofeng Jiang Jingwen Liang Masahito Fujio Elin Hadler-Olsen Jing Wang Alpdogan Kantarci Ying Xue |
| author_sort | Zhe Xing |
| collection | DOAJ |
| description | <b>Objective:</b> To evaluate the drug release, cytocompatibility with periodontal ligament cells (PDLCs), and therapeutic efficacy of GelMA hydrogel loaded with resolvin D1 (RvD1) in treating rat periodontal inflammation and alveolar bone damage. <b>Methods:</b> An RvD1 complexed with GelMA was prepared, and its release kinetics and compatibility with PDLCs were assessed. Rats with induced periodontitis were treated weekly with topical applications of vehicle, GelMA, RvD1, or RvD1 complexed with GelMA for four weeks. Periodontal inflammation and tissue regeneration were evaluated using quantitative PCR (qPCR) and histochemical staining, while alveolar bone repair and regeneration were analyzed through micro-CT. <b>Results:</b> The RvD1 complexed with GelMA effectively released RvD1 and enhanced the proliferation and viability of PDLCs. Compared to RvD1 alone, treatment with RvD1 complexed with GelMA significantly reduced inflammatory cell infiltration, TNF-α and RANKL expression, and osteoclast formation in periodontal tissues. Additionally, it promoted the expression of specific anti-inflammatory and regenerative markers. Micro-CT analysis confirmed significant new bone formation in the RvD1 complexed with GelMA-treated group. <b>Conclusions:</b> RvD1 complexed with GelMA provides sustained drug release and biocompatibility, effectively resolves periodontal inflammation, and promotes tissue regeneration in periodontitis. |
| format | Article |
| id | doaj-art-ed46a24addbc43649620fc2ff6ea0dd4 |
| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj-art-ed46a24addbc43649620fc2ff6ea0dd42025-01-24T13:45:35ZengMDPI AGPharmaceutics1999-49232024-12-011711610.3390/pharmaceutics17010016The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis ModelZhe Xing0Jing Liu1Jiazheng Cai2Xiaofeng Jiang3Jingwen Liang4Masahito Fujio5Elin Hadler-Olsen6Jing Wang7Alpdogan Kantarci8Ying Xue9School of Stomatology, Lanzhou University, Lanzhou 730000, ChinaSchool of Stomatology, Lanzhou University, Lanzhou 730000, ChinaSchool of Stomatology, Lanzhou University, Lanzhou 730000, ChinaSchool of Stomatology, Lanzhou University, Lanzhou 730000, ChinaSchool of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment of Oral and Maxillofacial Surgery, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, JapanDepartment of Medical Biology, Faculty of Health Sciences, UiT the Artic University of Norway, 9037 Tromsø, NorwaySchool of Stomatology, Lanzhou University, Lanzhou 730000, ChinaThe Forsyth Institute, Cambridge, MA 02142, USADepartment of Clinical Dentistry, Faculty of Health Sciences, UiT the Artic University of Norway, 9037 Tromsø, Norway<b>Objective:</b> To evaluate the drug release, cytocompatibility with periodontal ligament cells (PDLCs), and therapeutic efficacy of GelMA hydrogel loaded with resolvin D1 (RvD1) in treating rat periodontal inflammation and alveolar bone damage. <b>Methods:</b> An RvD1 complexed with GelMA was prepared, and its release kinetics and compatibility with PDLCs were assessed. Rats with induced periodontitis were treated weekly with topical applications of vehicle, GelMA, RvD1, or RvD1 complexed with GelMA for four weeks. Periodontal inflammation and tissue regeneration were evaluated using quantitative PCR (qPCR) and histochemical staining, while alveolar bone repair and regeneration were analyzed through micro-CT. <b>Results:</b> The RvD1 complexed with GelMA effectively released RvD1 and enhanced the proliferation and viability of PDLCs. Compared to RvD1 alone, treatment with RvD1 complexed with GelMA significantly reduced inflammatory cell infiltration, TNF-α and RANKL expression, and osteoclast formation in periodontal tissues. Additionally, it promoted the expression of specific anti-inflammatory and regenerative markers. Micro-CT analysis confirmed significant new bone formation in the RvD1 complexed with GelMA-treated group. <b>Conclusions:</b> RvD1 complexed with GelMA provides sustained drug release and biocompatibility, effectively resolves periodontal inflammation, and promotes tissue regeneration in periodontitis.https://www.mdpi.com/1999-4923/17/1/16periodontitisRvD1 complexed with GelMAdrug releaseinflammationtissue regeneration |
| spellingShingle | Zhe Xing Jing Liu Jiazheng Cai Xiaofeng Jiang Jingwen Liang Masahito Fujio Elin Hadler-Olsen Jing Wang Alpdogan Kantarci Ying Xue The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model Pharmaceutics periodontitis RvD1 complexed with GelMA drug release inflammation tissue regeneration |
| title | The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model |
| title_full | The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model |
| title_fullStr | The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model |
| title_full_unstemmed | The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model |
| title_short | The Application of Resolvin D1-Loaded Gelatin Methacrylate in a Rat Periodontitis Model |
| title_sort | application of resolvin d1 loaded gelatin methacrylate in a rat periodontitis model |
| topic | periodontitis RvD1 complexed with GelMA drug release inflammation tissue regeneration |
| url | https://www.mdpi.com/1999-4923/17/1/16 |
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