Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop
Phytopathogenic <i>Sclerotinia minor</i> Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples from fields containing <i>S. minor</i> Jagger were identified using in...
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2025-01-01
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| author | Lihui Xu Qinghua Shang Mogens Nicolaisen Rong Zeng Shigang Gao Ping Gao Zhiwei Song Fuming Dai Jingze Zhang |
| author_facet | Lihui Xu Qinghua Shang Mogens Nicolaisen Rong Zeng Shigang Gao Ping Gao Zhiwei Song Fuming Dai Jingze Zhang |
| author_sort | Lihui Xu |
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| description | Phytopathogenic <i>Sclerotinia minor</i> Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples from fields containing <i>S. minor</i> Jagger were identified using in vitro antagonistic assays against <i>S. minor</i> Jagger. Bioactivity experiments showed that Bac20 had higher inhibitory activity against <i>S. minor</i> Jagger than Bac45. Based on 16S rRNA sequences and phylogenetic analysis of a combination of sequences from <i>gyrA</i>, <i>rpoB</i>, <i>purH</i>, <i>polC</i>, and <i>groEL</i>, Bac20 and Bac45 were identified as <i>Bacillus velezensis</i> and <i>Bacillus subtilis</i>, respectively. Lipopeptide compounds produced by each strain were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI–TOF MS) analysis. Both strains produced three types of lipopeptides, namely surfactins, iturins, and fengycins, whereas Bac20 showed the strongest intensity in its production of iturins, more than that of Bac45. Bac20 inhibited oxalic acid formation in early-stage lettuce leaves infected with <i>S. minor</i> Jagger, delaying pathogen infestation. Greenhouse experiments for controlling lettuce drop demonstrated that inoculation with Bac20 controlled lettuce drop by 71.7%. In conclusion, this study revealed that <i>B. velezensis</i> Bac20 has high potential for use as a biocontrol agent for controlling the lettuce drop caused by <i>S. minor</i> Jagger. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Microorganisms |
| spelling | doaj-art-ed24c0f4acf24cbfb9ec5895fdba78882025-01-24T13:42:30ZengMDPI AGMicroorganisms2076-26072025-01-011316810.3390/microorganisms13010068Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce DropLihui Xu0Qinghua Shang1Mogens Nicolaisen2Rong Zeng3Shigang Gao4Ping Gao5Zhiwei Song6Fuming Dai7Jingze Zhang8Institute of Eco-Environmental Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Agroecology, Faculty of Technical Sciences, Aarhus University, 4200 Slagelse, DenmarkInstitute of Eco-Environmental Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Eco-Environmental Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Eco-Environmental Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Eco-Environmental Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Eco-Environmental Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaPhytopathogenic <i>Sclerotinia minor</i> Jagger causes lettuce drop, a destructive soil-borne disease. As potential biocontrol agents for this disease, 2 of 31 bacterial strains isolated from soil samples from fields containing <i>S. minor</i> Jagger were identified using in vitro antagonistic assays against <i>S. minor</i> Jagger. Bioactivity experiments showed that Bac20 had higher inhibitory activity against <i>S. minor</i> Jagger than Bac45. Based on 16S rRNA sequences and phylogenetic analysis of a combination of sequences from <i>gyrA</i>, <i>rpoB</i>, <i>purH</i>, <i>polC</i>, and <i>groEL</i>, Bac20 and Bac45 were identified as <i>Bacillus velezensis</i> and <i>Bacillus subtilis</i>, respectively. Lipopeptide compounds produced by each strain were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI–TOF MS) analysis. Both strains produced three types of lipopeptides, namely surfactins, iturins, and fengycins, whereas Bac20 showed the strongest intensity in its production of iturins, more than that of Bac45. Bac20 inhibited oxalic acid formation in early-stage lettuce leaves infected with <i>S. minor</i> Jagger, delaying pathogen infestation. Greenhouse experiments for controlling lettuce drop demonstrated that inoculation with Bac20 controlled lettuce drop by 71.7%. In conclusion, this study revealed that <i>B. velezensis</i> Bac20 has high potential for use as a biocontrol agent for controlling the lettuce drop caused by <i>S. minor</i> Jagger.https://www.mdpi.com/2076-2607/13/1/68antagonism<i>Bacillus</i>biocontrollettuce drop<i>Sclerotinia minor</i> Jagger |
| spellingShingle | Lihui Xu Qinghua Shang Mogens Nicolaisen Rong Zeng Shigang Gao Ping Gao Zhiwei Song Fuming Dai Jingze Zhang Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop Microorganisms antagonism <i>Bacillus</i> biocontrol lettuce drop <i>Sclerotinia minor</i> Jagger |
| title | Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop |
| title_full | Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop |
| title_fullStr | Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop |
| title_full_unstemmed | Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop |
| title_short | Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop |
| title_sort | biocontrol potential of rhizospheric bacillus strains against i sclerotinia minor i jagger causing lettuce drop |
| topic | antagonism <i>Bacillus</i> biocontrol lettuce drop <i>Sclerotinia minor</i> Jagger |
| url | https://www.mdpi.com/2076-2607/13/1/68 |
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