Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on Cells
Nanoparticles (NPs) have been introduced as a suitable alternative in many in vivo bioapplications. The risks of utilizing nanoparticles continue to be an ongoing research. Furthermore, the various chemicals used in their synthesis influence the cytotoxic effects of nanoparticles. We have investigat...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2014/631248 |
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| author | Smitha Rao Chienwen Huang Uday Tata Peter Wu Nikhil Arora Jinsung Ahn Victor K. Lin Yaowu Hao J.-C. Chiao |
| author_facet | Smitha Rao Chienwen Huang Uday Tata Peter Wu Nikhil Arora Jinsung Ahn Victor K. Lin Yaowu Hao J.-C. Chiao |
| author_sort | Smitha Rao |
| collection | DOAJ |
| description | Nanoparticles (NPs) have been introduced as a suitable alternative in many in vivo bioapplications. The risks of utilizing nanoparticles continue to be an ongoing research. Furthermore, the various chemicals used in their synthesis influence the cytotoxic effects of nanoparticles. We have investigated the cytotoxicity of Porous Hollow Au Nanoparticles (PHAuNPs) on cancer cell lines PC-3, PC-3ML, and MDA-MB-231 and the normal cell line PNT1A. Cell proliferation for the different cells in the presence of different concentrations of the PHAuNPs was assessed after 24 hours and 72 hours of incubation using MTT assay. The study also included the cytotoxic evaluation of pegylated PHAuNPs. Identical cell seeding densities, particle concentrations, and incubation times were employed for these two types of Au nanoparticles. Our results indicated that (1) impact on cell proliferation was concentration dependent and was different for the different cell types without cellular necrosis and (b) cellular proliferation might be impacted more based on the cell line. |
| format | Article |
| id | doaj-art-ed20489d93374213837efed055cacc9f |
| institution | OA Journals |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-ed20489d93374213837efed055cacc9f2025-08-20T02:22:06ZengWileyJournal of Nanotechnology1687-95031687-95112014-01-01201410.1155/2014/631248631248Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on CellsSmitha Rao0Chienwen Huang1Uday Tata2Peter Wu3Nikhil Arora4Jinsung Ahn5Victor K. Lin6Yaowu Hao7J.-C. Chiao8Department of Electrical Engineering, UT Arlington, 416 Yates Street, P.O. Box 19016, Arlington, TX 76019, USADepartment of Materials Science and Engineering, UT Arlington, 501 West 1st Street, P.O. Box 19031, Arlington, TX 76019, USADepartment of Electrical Engineering, UT Arlington, 416 Yates Street, P.O. Box 19016, Arlington, TX 76019, USATexas Academy of Math and Science, Denton, TX 76203, USALamar High School, Arlington, TX 76012, USAArlington High School, Arlington, TX 76013, USADepartment of Electrical Engineering, UT Arlington, 416 Yates Street, P.O. Box 19016, Arlington, TX 76019, USADepartment of Materials Science and Engineering, UT Arlington, 501 West 1st Street, P.O. Box 19031, Arlington, TX 76019, USADepartment of Electrical Engineering, UT Arlington, 416 Yates Street, P.O. Box 19016, Arlington, TX 76019, USANanoparticles (NPs) have been introduced as a suitable alternative in many in vivo bioapplications. The risks of utilizing nanoparticles continue to be an ongoing research. Furthermore, the various chemicals used in their synthesis influence the cytotoxic effects of nanoparticles. We have investigated the cytotoxicity of Porous Hollow Au Nanoparticles (PHAuNPs) on cancer cell lines PC-3, PC-3ML, and MDA-MB-231 and the normal cell line PNT1A. Cell proliferation for the different cells in the presence of different concentrations of the PHAuNPs was assessed after 24 hours and 72 hours of incubation using MTT assay. The study also included the cytotoxic evaluation of pegylated PHAuNPs. Identical cell seeding densities, particle concentrations, and incubation times were employed for these two types of Au nanoparticles. Our results indicated that (1) impact on cell proliferation was concentration dependent and was different for the different cell types without cellular necrosis and (b) cellular proliferation might be impacted more based on the cell line.http://dx.doi.org/10.1155/2014/631248 |
| spellingShingle | Smitha Rao Chienwen Huang Uday Tata Peter Wu Nikhil Arora Jinsung Ahn Victor K. Lin Yaowu Hao J.-C. Chiao Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on Cells Journal of Nanotechnology |
| title | Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on Cells |
| title_full | Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on Cells |
| title_fullStr | Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on Cells |
| title_full_unstemmed | Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on Cells |
| title_short | Evaluation of Cytotoxic Effects of Different Concentrations of Porous Hollow Au Nanoparticles (PHAuNPs) on Cells |
| title_sort | evaluation of cytotoxic effects of different concentrations of porous hollow au nanoparticles phaunps on cells |
| url | http://dx.doi.org/10.1155/2014/631248 |
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