L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in rats
Constipation is a common gastrointestinal disorder, and while L-arabinose has been shown to relieve it, the exact molecular mechanisms remain unclear. This study investigated the effects of L-arabinose in constipated rat at doses of 400, 600, and 800 mg/kg body weight (B.W.). The 800 mg/kg B.W. dose...
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000398 |
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| author | Ting Gong Linzheng Liao Yong Tang Weiwei Liu Ling Yao Zhen Wu Jianmei Li Fulai Bai Qian Zhang Liling Tang |
| author_facet | Ting Gong Linzheng Liao Yong Tang Weiwei Liu Ling Yao Zhen Wu Jianmei Li Fulai Bai Qian Zhang Liling Tang |
| author_sort | Ting Gong |
| collection | DOAJ |
| description | Constipation is a common gastrointestinal disorder, and while L-arabinose has been shown to relieve it, the exact molecular mechanisms remain unclear. This study investigated the effects of L-arabinose in constipated rat at doses of 400, 600, and 800 mg/kg body weight (B.W.). The 800 mg/kg B.W. dose significantly improved overall physiological state, stool consistency, intestinal transit, and gastric emptying. It also improved gastric, liver, and kidney function, while modulating key gastrointestinal peptides, including motilin (MTL), substance P (SP), vasoactive intestinal peptide (VIP), acetylcholinesterase (AchE), endothelin-1 (ET-1), serotonin (5-HT), somatostatin (SS), gastrin (Gas), and nitric oxide (NO). In addition, the 800 mg/kg dose notably increased goblet cell numbers, mucus layer thickness, and beneficial gut microbes such as Ruminococcus flavefaciens and Muribaculum intestinale. It also upregulated the expression of Cd38, Phlpp2, Muc3, and Acat2, while downregulating Cd34, C1qa, and C1qc. These results indicate that L-arabinose not only alleviates constipation but also enhances immune function and regulates signaling pathways, restoring gastrointestinal peptides to near-normal levels. |
| format | Article |
| id | doaj-art-ed1f271fb9c84b35ba28d0651947e8f5 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-ed1f271fb9c84b35ba28d0651947e8f52025-02-09T04:59:59ZengElsevierJournal of Functional Foods1756-46462025-02-01125106697L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in ratsTing Gong0Linzheng Liao1Yong Tang2Weiwei Liu3Ling Yao4Zhen Wu5Jianmei Li6Fulai Bai7Qian Zhang8Liling Tang9Chongqing University of Chinese Medicine, Institude of Intelligent Chinese Medicine, Chongqing 402760, ChinaChongqing University of Arts and Science, Horticultural Plant Engineering Research Center of Chongqing Universities, Chongqing, ChinaChongqing University of Chinese Medicine, Institude of Intelligent Chinese Medicine, Chongqing 402760, ChinaChongqing University of Chinese Medicine, Institude of Intelligent Chinese Medicine, Chongqing 402760, ChinaChongqing University of Chinese Medicine, Institude of Intelligent Chinese Medicine, Chongqing 402760, ChinaChongqing Key Laboratory of Innovative Traditional Chinese Medicine and Health Intervention, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, ChinaChongqing University of Chinese Medicine, Institude of Intelligent Chinese Medicine, Chongqing 402760, ChinaHealtang Biotech Co., Ltd. in China, Shandong, ChinaHealtang Biotech Co., Ltd. in China, Shandong, ChinaChongqing University of Chinese Medicine, Institude of Intelligent Chinese Medicine, Chongqing 402760, China; Corresponding author at: Chongqing University of Chinese Medicine, No. 61 Bicheng Road, Bishan District, Chongqing, 402760, China.Constipation is a common gastrointestinal disorder, and while L-arabinose has been shown to relieve it, the exact molecular mechanisms remain unclear. This study investigated the effects of L-arabinose in constipated rat at doses of 400, 600, and 800 mg/kg body weight (B.W.). The 800 mg/kg B.W. dose significantly improved overall physiological state, stool consistency, intestinal transit, and gastric emptying. It also improved gastric, liver, and kidney function, while modulating key gastrointestinal peptides, including motilin (MTL), substance P (SP), vasoactive intestinal peptide (VIP), acetylcholinesterase (AchE), endothelin-1 (ET-1), serotonin (5-HT), somatostatin (SS), gastrin (Gas), and nitric oxide (NO). In addition, the 800 mg/kg dose notably increased goblet cell numbers, mucus layer thickness, and beneficial gut microbes such as Ruminococcus flavefaciens and Muribaculum intestinale. It also upregulated the expression of Cd38, Phlpp2, Muc3, and Acat2, while downregulating Cd34, C1qa, and C1qc. These results indicate that L-arabinose not only alleviates constipation but also enhances immune function and regulates signaling pathways, restoring gastrointestinal peptides to near-normal levels.http://www.sciencedirect.com/science/article/pii/S1756464625000398ConstipationL-arabinoseGastrointestinal peptidesGut microbiotaTranscriptomics |
| spellingShingle | Ting Gong Linzheng Liao Yong Tang Weiwei Liu Ling Yao Zhen Wu Jianmei Li Fulai Bai Qian Zhang Liling Tang L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in rats Journal of Functional Foods Constipation L-arabinose Gastrointestinal peptides Gut microbiota Transcriptomics |
| title | L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in rats |
| title_full | L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in rats |
| title_fullStr | L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in rats |
| title_full_unstemmed | L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in rats |
| title_short | L-arabinose alleviates constipation through gastrointestinal peptide，gut microbiota，and Phlpp2 of gastrointestinal rhythms in rats |
| title_sort | l arabinose alleviates constipation through gastrointestinal peptide gut microbiota and phlpp2 of gastrointestinal rhythms in rats |
| topic | Constipation L-arabinose Gastrointestinal peptides Gut microbiota Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000398 |
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