Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep
Abstract Streptococcus dysgalactiae (S. dysgalactiae ) is a common pathogen of humans and various animals. However, the phylogenetic position of animal S. dysgalactiae isolates and their zoonotic potential remain unclear. Most molecular epidemiological studies explicate beta-hemolytic streptococci a...
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Nature Portfolio
2025-01-01
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| author | Ilona Stefańska Ewelina Kwiecień Anna Didkowska Magdalena Kizerwetter-Świda Dorota Chrobak-Chmiel Agnieszka Sałamaszyńska-Guz Piotr Żmuda Krzysztof Anusz Magdalena Rzewuska |
| author_facet | Ilona Stefańska Ewelina Kwiecień Anna Didkowska Magdalena Kizerwetter-Świda Dorota Chrobak-Chmiel Agnieszka Sałamaszyńska-Guz Piotr Żmuda Krzysztof Anusz Magdalena Rzewuska |
| author_sort | Ilona Stefańska |
| collection | DOAJ |
| description | Abstract Streptococcus dysgalactiae (S. dysgalactiae ) is a common pathogen of humans and various animals. However, the phylogenetic position of animal S. dysgalactiae isolates and their zoonotic potential remain unclear. Most molecular epidemiological studies explicate beta-hemolytic streptococci according to their MLST and M protein gene (emm) types. Although human S. dysgalactiae isolates are relatively well characterized, the data concerning animal isolates are scarce. Here, we report the molecular characteristics and antimicrobial resistance of S. dysgalactiae strains recovered from sheep and their genetic relationship with isolates from other animal hosts and humans. Overall, 11 PFGE pulsotypes, five MLST sequence types (STs), and two emm types were distinguished, with ST248 and stL1376 being the most prevalent, indicating genetic diversity among tested 17 ovine isolates. Some isolates exhibited resistance to doxycycline (59%), erythromycin (6%), ciprofloxacin (6%), and trimethoprim/sulfamethoxazole (6%), harboring various resistance determinants. Phylogenetic analysis showed that studied ovine isolates grouped together with human S. dysgalactiae isolates from the cases of zoonotic infections. Moreover, some ovine isolates shared identical STs and emm gene sequences with human non-invasive and invasive S. dysgalactiae strains. These findings suggest a possible link between human and ovine isolates and indicate the zoonotic potential of this pathogen. |
| format | Article |
| id | doaj-art-ed150cf8c0674860ba033cadcc4c2270 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-ed150cf8c0674860ba033cadcc4c22702025-01-26T12:28:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-87781-3Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheepIlona Stefańska0Ewelina Kwiecień1Anna Didkowska2Magdalena Kizerwetter-Świda3Dorota Chrobak-Chmiel4Agnieszka Sałamaszyńska-Guz5Piotr Żmuda6Krzysztof Anusz7Magdalena Rzewuska8Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesUniversity Centre of Veterinary Medicine, University of Agriculture in KrakowDepartment of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life SciencesAbstract Streptococcus dysgalactiae (S. dysgalactiae ) is a common pathogen of humans and various animals. However, the phylogenetic position of animal S. dysgalactiae isolates and their zoonotic potential remain unclear. Most molecular epidemiological studies explicate beta-hemolytic streptococci according to their MLST and M protein gene (emm) types. Although human S. dysgalactiae isolates are relatively well characterized, the data concerning animal isolates are scarce. Here, we report the molecular characteristics and antimicrobial resistance of S. dysgalactiae strains recovered from sheep and their genetic relationship with isolates from other animal hosts and humans. Overall, 11 PFGE pulsotypes, five MLST sequence types (STs), and two emm types were distinguished, with ST248 and stL1376 being the most prevalent, indicating genetic diversity among tested 17 ovine isolates. Some isolates exhibited resistance to doxycycline (59%), erythromycin (6%), ciprofloxacin (6%), and trimethoprim/sulfamethoxazole (6%), harboring various resistance determinants. Phylogenetic analysis showed that studied ovine isolates grouped together with human S. dysgalactiae isolates from the cases of zoonotic infections. Moreover, some ovine isolates shared identical STs and emm gene sequences with human non-invasive and invasive S. dysgalactiae strains. These findings suggest a possible link between human and ovine isolates and indicate the zoonotic potential of this pathogen.https://doi.org/10.1038/s41598-025-87781-3Beta-hemolytic streptococciMultilocus sequence typingM protein gene (emm) typingSheepStreptococcus dysgalactiaeZoonotic pathogen |
| spellingShingle | Ilona Stefańska Ewelina Kwiecień Anna Didkowska Magdalena Kizerwetter-Świda Dorota Chrobak-Chmiel Agnieszka Sałamaszyńska-Guz Piotr Żmuda Krzysztof Anusz Magdalena Rzewuska Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep Scientific Reports Beta-hemolytic streptococci Multilocus sequence typing M protein gene (emm) typing Sheep Streptococcus dysgalactiae Zoonotic pathogen |
| title | Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep |
| title_full | Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep |
| title_fullStr | Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep |
| title_full_unstemmed | Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep |
| title_short | Genetic analysis reveals the genetic diversity and zoonotic potential of Streptococcus dysgalactiae isolates from sheep |
| title_sort | genetic analysis reveals the genetic diversity and zoonotic potential of streptococcus dysgalactiae isolates from sheep |
| topic | Beta-hemolytic streptococci Multilocus sequence typing M protein gene (emm) typing Sheep Streptococcus dysgalactiae Zoonotic pathogen |
| url | https://doi.org/10.1038/s41598-025-87781-3 |
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