Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cells
Arsenic is a pervasive environmental pollutant that can bioaccumulate in Antarctic krill through the food chain, posing potential risks to human health. This study investigates the toxic effects of arsenic in Antarctic krill oil (AKO) on Caco-2 cells, focusing on oxidative stress and apoptosis induc...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000168 |
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| author | Haiyan Zhang Yunyun Ji Zhongquan Jiang Guangxin Yang Cong Kong Zhemin Shen Tao Yuan Xiaosheng Shen |
| author_facet | Haiyan Zhang Yunyun Ji Zhongquan Jiang Guangxin Yang Cong Kong Zhemin Shen Tao Yuan Xiaosheng Shen |
| author_sort | Haiyan Zhang |
| collection | DOAJ |
| description | Arsenic is a pervasive environmental pollutant that can bioaccumulate in Antarctic krill through the food chain, posing potential risks to human health. This study investigates the toxic effects of arsenic in Antarctic krill oil (AKO) on Caco-2 cells, focusing on oxidative stress and apoptosis induction. AKO is nutrient-rich and contains various arsenic species, including arsenite (As³⁺), arsenate (As⁵⁺), dimethyl arsinic acid (DMA), and arsenobetaine (AsB), each exhibiting different toxic potencies. Caco-2 cells were treated with arsenic standards and AKO to assess cell viability, lactate dehydrogenase (LDH) release, oxidative stress markers (superoxide dismutase [SOD], catalase [CAT], malondialdehyde [MDA], and glutathione peroxidase [GSH-Px]), reactive oxygen species (ROS) production, and apoptosis. Results demonstrated dose-dependent cytotoxicity, with As³ ⁺ being the most toxic, followed by As⁵⁺, DMA, and AsB. After 24 hours of exposure, cell viability in the high-concentration AKO group decreased to 63.95 %. Arsenic exposure elevated ROS levels, disrupted mitochondrial membrane potential, upregulated apoptosis-related genes such as Caspase-3, Caspase-9, and Bax, and downregulated the PI3K/AKT/mTOR signaling pathway. This study elucidates the mechanisms underlying arsenic toxicity in AKO and underscores its implications for food safety assessments. |
| format | Article |
| id | doaj-art-ecf0638a990042e485af411122cc7e8a |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-ecf0638a990042e485af411122cc7e8a2025-01-23T05:26:05ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117680Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cellsHaiyan Zhang0Yunyun Ji1Zhongquan Jiang2Guangxin Yang3Cong Kong4Zhemin Shen5Tao Yuan6Xiaosheng Shen7East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Ministry of Ecology and Environment, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaKey Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Ministry of Ecology and Environment, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaKey Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Ministry of Ecology and Environment, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding authors.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; Corresponding authors.Arsenic is a pervasive environmental pollutant that can bioaccumulate in Antarctic krill through the food chain, posing potential risks to human health. This study investigates the toxic effects of arsenic in Antarctic krill oil (AKO) on Caco-2 cells, focusing on oxidative stress and apoptosis induction. AKO is nutrient-rich and contains various arsenic species, including arsenite (As³⁺), arsenate (As⁵⁺), dimethyl arsinic acid (DMA), and arsenobetaine (AsB), each exhibiting different toxic potencies. Caco-2 cells were treated with arsenic standards and AKO to assess cell viability, lactate dehydrogenase (LDH) release, oxidative stress markers (superoxide dismutase [SOD], catalase [CAT], malondialdehyde [MDA], and glutathione peroxidase [GSH-Px]), reactive oxygen species (ROS) production, and apoptosis. Results demonstrated dose-dependent cytotoxicity, with As³ ⁺ being the most toxic, followed by As⁵⁺, DMA, and AsB. After 24 hours of exposure, cell viability in the high-concentration AKO group decreased to 63.95 %. Arsenic exposure elevated ROS levels, disrupted mitochondrial membrane potential, upregulated apoptosis-related genes such as Caspase-3, Caspase-9, and Bax, and downregulated the PI3K/AKT/mTOR signaling pathway. This study elucidates the mechanisms underlying arsenic toxicity in AKO and underscores its implications for food safety assessments.http://www.sciencedirect.com/science/article/pii/S0147651325000168Antarctic krill oilArsenic speciesCaco-2 cellsOxidative stressApoptosis |
| spellingShingle | Haiyan Zhang Yunyun Ji Zhongquan Jiang Guangxin Yang Cong Kong Zhemin Shen Tao Yuan Xiaosheng Shen Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cells Ecotoxicology and Environmental Safety Antarctic krill oil Arsenic species Caco-2 cells Oxidative stress Apoptosis |
| title | Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cells |
| title_full | Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cells |
| title_fullStr | Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cells |
| title_full_unstemmed | Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cells |
| title_short | Arsenic toxicity in Antarctic krill oil and its impact on human intestinal cells |
| title_sort | arsenic toxicity in antarctic krill oil and its impact on human intestinal cells |
| topic | Antarctic krill oil Arsenic species Caco-2 cells Oxidative stress Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000168 |
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