Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1
Objective To investigate the effects of artesunate (Art) on the proliferation, apoptosis, and autophagy of nephroblastoma cell line (SK-NEP-1). Methods SK-NEP-1 cells were intervened with different concentrations of Art (0, 10, 20, 40 and 80 μmol/L), and MTT method was applied to calculate the cel...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-04-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-4-493.pdf |
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| author | WEI Jianxin, FANG Yanle, LU Yubo, GAO Yuguang, LANG Xing, LI Jingtao, MA Xinsheng |
| author_facet | WEI Jianxin, FANG Yanle, LU Yubo, GAO Yuguang, LANG Xing, LI Jingtao, MA Xinsheng |
| author_sort | WEI Jianxin, FANG Yanle, LU Yubo, GAO Yuguang, LANG Xing, LI Jingtao, MA Xinsheng |
| collection | DOAJ |
| description | Objective To investigate the effects of artesunate (Art) on the proliferation, apoptosis, and autophagy of nephroblastoma cell line (SK-NEP-1). Methods SK-NEP-1 cells were intervened with different concentrations of Art (0, 10, 20, 40 and 80 μmol/L), and MTT method was applied to calculate the cell proliferation inhibition rate to screen the optimal intervention concentration; SK-NEP-1 cells were separated into control group, Art group, 3-MA group (Art+autophagy inhibitor, 3-methyladenine), and Rapa group (Art+autophagy activator rapamycin). EdU and flow cytometry were applied to detect cell proliferation and apoptosis, respectively; MDC staining was applied to detect autophagy in cells; the level of reactive oxygen species (ROS) in cells was detected by DCFH-DA fluorescent probe; the expression of proliferating cell nuclear antigen (PCNA), anti apoptotic factor B cell lymphomatoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), microtubule junction protein 1 light chain 3Ⅱ/3Ⅰ (LC3Ⅱ/LC3Ⅰ), selective autophagy junction protein 1 (p62), and benzyl chloride 1 (Beclin-1) proteins in cells were detected by Western blot. Results Compared with 0 μmol/L Art, the proliferation inhibition rate of SK-NEP-1 cells was gradually increased after 10, 20, 40 and 80 μmol/L Art treatment (P＜0.05), and the IC50 value was 46.881 μmol/L, so 40 μmol/L Art was selected for follow-up experiments. Compared with the control group, the apoptosis rate, relative autophagy fluorescence intensity, ROS level, Bax, LC3 Ⅱ/LC3 Ⅰ, Beclin-1, PINK1, and Parkin protein expression levels of SK-NEP-1 cells in the Art group were obviously increased, the EdU positive cell rate, PCNA, Bcl-2, and P62 protein expression levels were obviously reduced (P＜0.05); The autophagy inhibitor 3-MA inhibited the promoting effect of Art on apoptosis and autophagy of nephroblastoma cells and inhibit proliferation (P＜0.05). Conclusions Art inhibits the proliferation of nephroblastoma cell line SK-NEP-1, and promotes autophagy and apoptosis. |
| format | Article |
| id | doaj-art-ecee48e52b1f447899abaab02e89d0ef |
| institution | Kabale University |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-04-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-ecee48e52b1f447899abaab02e89d0ef2025-08-20T03:44:33ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-04-0145449349810.16352/j.issn.1001-6325.2025.04.0493Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1WEI Jianxin, FANG Yanle, LU Yubo, GAO Yuguang, LANG Xing, LI Jingtao, MA Xinsheng01. the Second Department of Pediatric Surgery, Handan Central Hospital, Handan 056000;;2. the Third Department of General Surgery, the Second Hospital of Hebei Medical University, Shijiazhuang 050000;;3. Department of Pharmacy, Handan Central Hospital, Handan 056000, ChinaObjective To investigate the effects of artesunate (Art) on the proliferation, apoptosis, and autophagy of nephroblastoma cell line (SK-NEP-1). Methods SK-NEP-1 cells were intervened with different concentrations of Art (0, 10, 20, 40 and 80 μmol/L), and MTT method was applied to calculate the cell proliferation inhibition rate to screen the optimal intervention concentration; SK-NEP-1 cells were separated into control group, Art group, 3-MA group (Art+autophagy inhibitor, 3-methyladenine), and Rapa group (Art+autophagy activator rapamycin). EdU and flow cytometry were applied to detect cell proliferation and apoptosis, respectively; MDC staining was applied to detect autophagy in cells; the level of reactive oxygen species (ROS) in cells was detected by DCFH-DA fluorescent probe; the expression of proliferating cell nuclear antigen (PCNA), anti apoptotic factor B cell lymphomatoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), microtubule junction protein 1 light chain 3Ⅱ/3Ⅰ (LC3Ⅱ/LC3Ⅰ), selective autophagy junction protein 1 (p62), and benzyl chloride 1 (Beclin-1) proteins in cells were detected by Western blot. Results Compared with 0 μmol/L Art, the proliferation inhibition rate of SK-NEP-1 cells was gradually increased after 10, 20, 40 and 80 μmol/L Art treatment (P＜0.05), and the IC50 value was 46.881 μmol/L, so 40 μmol/L Art was selected for follow-up experiments. Compared with the control group, the apoptosis rate, relative autophagy fluorescence intensity, ROS level, Bax, LC3 Ⅱ/LC3 Ⅰ, Beclin-1, PINK1, and Parkin protein expression levels of SK-NEP-1 cells in the Art group were obviously increased, the EdU positive cell rate, PCNA, Bcl-2, and P62 protein expression levels were obviously reduced (P＜0.05); The autophagy inhibitor 3-MA inhibited the promoting effect of Art on apoptosis and autophagy of nephroblastoma cells and inhibit proliferation (P＜0.05). Conclusions Art inhibits the proliferation of nephroblastoma cell line SK-NEP-1, and promotes autophagy and apoptosis.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-4-493.pdfartesunate|nephroblastoma|proliferation|apoptosis|autophagy |
| spellingShingle | WEI Jianxin, FANG Yanle, LU Yubo, GAO Yuguang, LANG Xing, LI Jingtao, MA Xinsheng Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1 Jichu yixue yu linchuang artesunate|nephroblastoma|proliferation|apoptosis|autophagy |
| title | Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1 |
| title_full | Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1 |
| title_fullStr | Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1 |
| title_full_unstemmed | Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1 |
| title_short | Artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line SK-NEP-1 |
| title_sort | artesunate inhibits proliferation and promotes apoptosis and autophagy of nephroblastoma cell line sk nep 1 |
| topic | artesunate|nephroblastoma|proliferation|apoptosis|autophagy |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-4-493.pdf |
| work_keys_str_mv | AT weijianxinfangyanleluyubogaoyuguanglangxinglijingtaomaxinsheng artesunateinhibitsproliferationandpromotesapoptosisandautophagyofnephroblastomacelllinesknep1 |