Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells
<i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene...
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2025-01-01
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| author | Hua He Yueyue Lin Xi Zhang Hengli Xie Zhujun Wang Shenqiang Hu Liang Li Hehe Liu Chunchun Han Lu Xia Jiwei Hu Jiwen Wang Lin Liao Xin Yuan |
| author_facet | Hua He Yueyue Lin Xi Zhang Hengli Xie Zhujun Wang Shenqiang Hu Liang Li Hehe Liu Chunchun Han Lu Xia Jiwei Hu Jiwen Wang Lin Liao Xin Yuan |
| author_sort | Hua He |
| collection | DOAJ |
| description | <i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched (<i>p</i> < 0.05) in pathways related to biological processes (BPs), particularly those associated with the regulation of cellular lipid metabolism and oxidative stress. KEGG analysis further identified significant enrichment (<i>p</i> < 0.05) in pathways related to cell apoptosis and the cell cycle. A joint analysis of KEGG and PPI on the upregulated and downregulated DEGs revealed that the TGF-β signaling pathway was the only pathway significantly enriched among both upregulated and downregulated DEGs after <i>PLIN1</i> overexpression in hGCs and phGCs. Based on these findings, we hypothesize that <i>PLIN1</i> overexpression may promote granulosa cell proliferation and apoptosis by activating the TGF-β signaling pathway in goose follicular GCs. Additionally, nine potential candidate genes were identified: <i>PPARγ</i>, <i>MGLL</i>, <i>PTEN</i>, <i>BAMBI</i>, <i>BMPR2</i>, <i>JUN</i>, <i>FST</i>, <i>ACSF3</i>, and <i>ACSL4</i>. These results address a significant research gap concerning the role of this gene in granulosa cells and contribute to the understanding of its molecular regulatory mechanisms. |
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| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Animals |
| spelling | doaj-art-ecec59c5e20841caa3d84be570f7a7882025-01-24T13:18:26ZengMDPI AGAnimals2076-26152025-01-0115228410.3390/ani15020284Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa CellsHua He0Yueyue Lin1Xi Zhang2Hengli Xie3Zhujun Wang4Shenqiang Hu5Liang Li6Hehe Liu7Chunchun Han8Lu Xia9Jiwei Hu10Jiwen Wang11Lin Liao12Xin Yuan13Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China<i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched (<i>p</i> < 0.05) in pathways related to biological processes (BPs), particularly those associated with the regulation of cellular lipid metabolism and oxidative stress. KEGG analysis further identified significant enrichment (<i>p</i> < 0.05) in pathways related to cell apoptosis and the cell cycle. A joint analysis of KEGG and PPI on the upregulated and downregulated DEGs revealed that the TGF-β signaling pathway was the only pathway significantly enriched among both upregulated and downregulated DEGs after <i>PLIN1</i> overexpression in hGCs and phGCs. Based on these findings, we hypothesize that <i>PLIN1</i> overexpression may promote granulosa cell proliferation and apoptosis by activating the TGF-β signaling pathway in goose follicular GCs. Additionally, nine potential candidate genes were identified: <i>PPARγ</i>, <i>MGLL</i>, <i>PTEN</i>, <i>BAMBI</i>, <i>BMPR2</i>, <i>JUN</i>, <i>FST</i>, <i>ACSF3</i>, and <i>ACSL4</i>. These results address a significant research gap concerning the role of this gene in granulosa cells and contribute to the understanding of its molecular regulatory mechanisms.https://www.mdpi.com/2076-2615/15/2/284<i>PLIN1</i>GCstranscriptomemolecular mechanismgoose |
| spellingShingle | Hua He Yueyue Lin Xi Zhang Hengli Xie Zhujun Wang Shenqiang Hu Liang Li Hehe Liu Chunchun Han Lu Xia Jiwei Hu Jiwen Wang Lin Liao Xin Yuan Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells Animals <i>PLIN1</i> GCs transcriptome molecular mechanism goose |
| title | Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells |
| title_full | Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells |
| title_fullStr | Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells |
| title_full_unstemmed | Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells |
| title_short | Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells |
| title_sort | transcriptome analysis reveals the molecular mechanism of plin1 in goose hierarchical and pre hierarchical follicle granulosa cells |
| topic | <i>PLIN1</i> GCs transcriptome molecular mechanism goose |
| url | https://www.mdpi.com/2076-2615/15/2/284 |
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