Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells

Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells

<i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene...

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Main Authors: Hua He, Yueyue Lin, Xi Zhang, Hengli Xie, Zhujun Wang, Shenqiang Hu, Liang Li, Hehe Liu, Chunchun Han, Lu Xia, Jiwei Hu, Jiwen Wang, Lin Liao, Xin Yuan
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Animals
Subjects:
<i>PLIN1</i>
GCs
transcriptome
molecular mechanism
goose
Online Access:https://www.mdpi.com/2076-2615/15/2/284
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Summary:<i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched (<i>p</i> < 0.05) in pathways related to biological processes (BPs), particularly those associated with the regulation of cellular lipid metabolism and oxidative stress. KEGG analysis further identified significant enrichment (<i>p</i> < 0.05) in pathways related to cell apoptosis and the cell cycle. A joint analysis of KEGG and PPI on the upregulated and downregulated DEGs revealed that the TGF-β signaling pathway was the only pathway significantly enriched among both upregulated and downregulated DEGs after <i>PLIN1</i> overexpression in hGCs and phGCs. Based on these findings, we hypothesize that <i>PLIN1</i> overexpression may promote granulosa cell proliferation and apoptosis by activating the TGF-β signaling pathway in goose follicular GCs. Additionally, nine potential candidate genes were identified: <i>PPARγ</i>, <i>MGLL</i>, <i>PTEN</i>, <i>BAMBI</i>, <i>BMPR2</i>, <i>JUN</i>, <i>FST</i>, <i>ACSF3</i>, and <i>ACSL4</i>. These results address a significant research gap concerning the role of this gene in granulosa cells and contribute to the understanding of its molecular regulatory mechanisms.
ISSN:2076-2615

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