Immunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 Patients
Background Diagnostic assays aimed at the identification of immunoglobulin G (IgG) and immunoglobulin M (IgM) offer a rapid and adjunct modality to conventional real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the diagnosis of coronavirus disease 2019 (COVID-19). Aim To...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-07-01
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| Series: | Dr. Sulaiman Al Habib Medical Journal |
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| Online Access: | https://journals.lww.com/10.1007/s44229-022-00014-x |
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| author | Ahmad A. Alharbi Mohammad K. Alshomrani Abdullah A. Alharbi Abdulrahman H. Almaeen Saad AlAsiri Awad Al-Omari Imad Alishat Saeed Dolgom |
| author_facet | Ahmad A. Alharbi Mohammad K. Alshomrani Abdullah A. Alharbi Abdulrahman H. Almaeen Saad AlAsiri Awad Al-Omari Imad Alishat Saeed Dolgom |
| author_sort | Ahmad A. Alharbi |
| collection | DOAJ |
| description | Background
Diagnostic assays aimed at the identification of immunoglobulin G (IgG) and immunoglobulin M (IgM) offer a rapid and adjunct modality to conventional real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the diagnosis of coronavirus disease 2019 (COVID-19).
Aim
To analyze the sensitivity of IgG and IgM-based serological assays in rRT-PCR-positive COVID-19 subjects.
Methods
A consecutive cohort of 69 patients with COVID-19-related symptoms or recent exposure to COVID-19-positive individuals were included after taking informed consent. Nasopharyngeal swabs for SARS-CoV-2 rRT-PCR analysis and venous blood samples for the COVID-19 IgG/IgM rapid test were simultaneously collected from each subject on day 0. Then, in the case of positive PCR results, subsequent blood samples for COVID-19 IgG/IgM analysis were collected on days 7, 10 and 14. Samples were statistically analyzed to determine the sensitivity of the serology-based assays.
Results
No correlation was found between age or sex and the rRT-PCR, IgG and IgM results; 65.2% of subjects tested positive by rRT-PCR. The sensitivity of the IgM and IgG rapid test increased gradually with time, reaching the highest level on day 14 (22.2% and 72%, respectively).
Conclusion
Serological assays for the detection of infection with SARS-CoV-2 were compared to rRT-PCR. These assays yielded lower sensitivities than rRT-PCR-based assays. However, given that these immunoassays are more affordable, faster, and easier to execute, they could be recommended for epidemiological research or characterizing the immune status of postinfection or post-vaccination subjects. |
| format | Article |
| id | doaj-art-ecea653341734fd091fc3b4d3e5efc38 |
| institution | Kabale University |
| issn | 2666-819X 2590-3349 |
| language | English |
| publishDate | 2022-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Dr. Sulaiman Al Habib Medical Journal |
| spelling | doaj-art-ecea653341734fd091fc3b4d3e5efc382025-08-20T03:36:42ZengWolters Kluwer Medknow PublicationsDr. Sulaiman Al Habib Medical Journal2666-819X2590-33492022-07-014315315810.1007/s44229-022-00014-xImmunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 PatientsAhmad A. AlharbiMohammad K. AlshomraniAbdullah A. AlharbiAbdulrahman H. AlmaeenSaad AlAsiriAwad Al-OmariImad AlishatSaeed DolgomBackground Diagnostic assays aimed at the identification of immunoglobulin G (IgG) and immunoglobulin M (IgM) offer a rapid and adjunct modality to conventional real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the diagnosis of coronavirus disease 2019 (COVID-19). Aim To analyze the sensitivity of IgG and IgM-based serological assays in rRT-PCR-positive COVID-19 subjects. Methods A consecutive cohort of 69 patients with COVID-19-related symptoms or recent exposure to COVID-19-positive individuals were included after taking informed consent. Nasopharyngeal swabs for SARS-CoV-2 rRT-PCR analysis and venous blood samples for the COVID-19 IgG/IgM rapid test were simultaneously collected from each subject on day 0. Then, in the case of positive PCR results, subsequent blood samples for COVID-19 IgG/IgM analysis were collected on days 7, 10 and 14. Samples were statistically analyzed to determine the sensitivity of the serology-based assays. Results No correlation was found between age or sex and the rRT-PCR, IgG and IgM results; 65.2% of subjects tested positive by rRT-PCR. The sensitivity of the IgM and IgG rapid test increased gradually with time, reaching the highest level on day 14 (22.2% and 72%, respectively). Conclusion Serological assays for the detection of infection with SARS-CoV-2 were compared to rRT-PCR. These assays yielded lower sensitivities than rRT-PCR-based assays. However, given that these immunoassays are more affordable, faster, and easier to execute, they could be recommended for epidemiological research or characterizing the immune status of postinfection or post-vaccination subjects.https://journals.lww.com/10.1007/s44229-022-00014-xcovid-19rapid serological testiggigmsensitivity |
| spellingShingle | Ahmad A. Alharbi Mohammad K. Alshomrani Abdullah A. Alharbi Abdulrahman H. Almaeen Saad AlAsiri Awad Al-Omari Imad Alishat Saeed Dolgom Immunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 Patients Dr. Sulaiman Al Habib Medical Journal covid-19 rapid serological test igg igm sensitivity |
| title | Immunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 Patients |
| title_full | Immunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 Patients |
| title_fullStr | Immunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 Patients |
| title_full_unstemmed | Immunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 Patients |
| title_short | Immunoglobulin Rapid Test Sensitivity in PCR-Positive COVID-19 Patients |
| title_sort | immunoglobulin rapid test sensitivity in pcr positive covid 19 patients |
| topic | covid-19 rapid serological test igg igm sensitivity |
| url | https://journals.lww.com/10.1007/s44229-022-00014-x |
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