Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico Studies
<p align="justify"><em>Candida</em> species are globally recognized for invasive infections with poor prognosis. Burgeoning quest for the discovery of novel therapeutics has increased the scientific scrutiny of several medicinal plants. This study assessed the efficacy of...
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Academy Publishing Center
2025-03-01
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| Series: | Advances in Medical, Pharmaceutical and Dental Research |
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| Online Access: | http://apc.aast.edu/ojs/index.php/AMPDR/article/view/1060 |
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| author | Olumide Oluyele Damilola Alex Omoboyowa Ayomide Esther Aderogba Koffi Adusa Osei |
| author_facet | Olumide Oluyele Damilola Alex Omoboyowa Ayomide Esther Aderogba Koffi Adusa Osei |
| author_sort | Olumide Oluyele |
| collection | DOAJ |
| description | <p align="justify"><em>Candida</em> species are globally recognized for invasive infections with poor prognosis. Burgeoning quest for the discovery of novel therapeutics has increased the scientific scrutiny of several medicinal plants. This study assessed the efficacy of <em>Piper guineense </em>crude extract and fractions against selected multi-drug resistant non- <em>albicans</em> <em>Candida</em> species. Both the crude and fractionated extracts of <em>P. guineense </em>elicited marked anti-candidal activity. Overall, the crude extract showed better efficacy over the fractions with the highest inhibition zone (25.0mm) recorded against <em>Candida tropicalis</em>; amongst the fractions, n-hexane fraction (F2) produced the highest inhibition zone (23mm) against <em>Candida glabrata</em>. The MIC ranged from 25mg/ml to 50mg/ml; while the MBC ranged between 100 mg/ml to 200 mg/ml. HPLC analysis revealed the presence of 14 compounds in the extract, with prominent members being quercetin, ellagic acid, persin, catechin, p-coumaric acid, and lutein. The binding affinity and free binding energy results reveal that most of these bioactive compounds were better than the standard drug (Fluconazole). Conclusively, <em>P. guineense</em> extracts demonstrated impressive anti-candidal properties against the tested multi-drug resistant non-<em>albicans</em> <em>Candida</em> species and could have potential as new drug lead for the treatment of infections resulting from these pathogens.</p><p align="justify"><strong>Received on, 17 October 2024</strong></p><p align="justify"><strong>Accepted on, 15 January 2025</strong></p><p align="justify"><strong>Published on, 15 March 2025</strong></p> |
| format | Article |
| id | doaj-art-ece9fd1e8c624695b6daef4be5a480d3 |
| institution | Kabale University |
| issn | 2812-488X 2812-4898 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Academy Publishing Center |
| record_format | Article |
| series | Advances in Medical, Pharmaceutical and Dental Research |
| spelling | doaj-art-ece9fd1e8c624695b6daef4be5a480d32025-08-20T03:28:06ZengAcademy Publishing CenterAdvances in Medical, Pharmaceutical and Dental Research2812-488X2812-48982025-03-0151102110.21622/AMPDR.2025.05.1.1060463Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico StudiesOlumide Oluyele0Damilola Alex Omoboyowa1Ayomide Esther Aderogba2Koffi Adusa Osei3Department of Microbiology, Adekunle Ajasin University Akungba-AkokoPhyto-medicine and Computational Biology Lab., Department of Biochemistry, Adekunle Ajasin University Akungba-AkokoDepartment of Microbiology, Adekunle Ajasin University Akungba-AkokoDepartment of Microbiology, Adekunle Ajasin University Akungba-Akoko<p align="justify"><em>Candida</em> species are globally recognized for invasive infections with poor prognosis. Burgeoning quest for the discovery of novel therapeutics has increased the scientific scrutiny of several medicinal plants. This study assessed the efficacy of <em>Piper guineense </em>crude extract and fractions against selected multi-drug resistant non- <em>albicans</em> <em>Candida</em> species. Both the crude and fractionated extracts of <em>P. guineense </em>elicited marked anti-candidal activity. Overall, the crude extract showed better efficacy over the fractions with the highest inhibition zone (25.0mm) recorded against <em>Candida tropicalis</em>; amongst the fractions, n-hexane fraction (F2) produced the highest inhibition zone (23mm) against <em>Candida glabrata</em>. The MIC ranged from 25mg/ml to 50mg/ml; while the MBC ranged between 100 mg/ml to 200 mg/ml. HPLC analysis revealed the presence of 14 compounds in the extract, with prominent members being quercetin, ellagic acid, persin, catechin, p-coumaric acid, and lutein. The binding affinity and free binding energy results reveal that most of these bioactive compounds were better than the standard drug (Fluconazole). Conclusively, <em>P. guineense</em> extracts demonstrated impressive anti-candidal properties against the tested multi-drug resistant non-<em>albicans</em> <em>Candida</em> species and could have potential as new drug lead for the treatment of infections resulting from these pathogens.</p><p align="justify"><strong>Received on, 17 October 2024</strong></p><p align="justify"><strong>Accepted on, 15 January 2025</strong></p><p align="justify"><strong>Published on, 15 March 2025</strong></p>http://apc.aast.edu/ojs/index.php/AMPDR/article/view/1060p. guineense, non-albicans candida, lanosterol- 14α-demethylase, antimicrobial resistance |
| spellingShingle | Olumide Oluyele Damilola Alex Omoboyowa Ayomide Esther Aderogba Koffi Adusa Osei Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico Studies Advances in Medical, Pharmaceutical and Dental Research p. guineense, non-albicans candida, lanosterol- 14α-demethylase, antimicrobial resistance |
| title | Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico Studies |
| title_full | Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico Studies |
| title_fullStr | Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico Studies |
| title_full_unstemmed | Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico Studies |
| title_short | Piper guineense (Swhan and Thon) Inhibits Lanosterol-14α-demethylase in Multi-Drug Resistant Non-albicans Candida Species: In vitro and In silico Studies |
| title_sort | piper guineense swhan and thon inhibits lanosterol 14α demethylase in multi drug resistant non albicans candida species in vitro and in silico studies |
| topic | p. guineense, non-albicans candida, lanosterol- 14α-demethylase, antimicrobial resistance |
| url | http://apc.aast.edu/ojs/index.php/AMPDR/article/view/1060 |
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