Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms
Zinc-supplementation (20 μM) effects on growth, photosynthesis, antioxidant enzyme activities (superoxide dismutase, ascorbate peroxidase, catalase), and the expression of phytochelatin synthase gene were investigated in four marine diatoms (Amphora acutiuscula, Nitzschia palea, Amphora coffeaeformi...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/982957 |
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| author | Thi Le Nhung Nguyen-Deroche Aurore Caruso Thi Trung Le Trang Viet Bui Benoît Schoefs Gérard Tremblin Annick Morant-Manceau |
| author_facet | Thi Le Nhung Nguyen-Deroche Aurore Caruso Thi Trung Le Trang Viet Bui Benoît Schoefs Gérard Tremblin Annick Morant-Manceau |
| author_sort | Thi Le Nhung Nguyen-Deroche |
| collection | DOAJ |
| description | Zinc-supplementation (20 μM) effects on growth, photosynthesis, antioxidant enzyme activities (superoxide dismutase, ascorbate peroxidase, catalase), and the expression of phytochelatin synthase gene were investigated in four marine diatoms (Amphora acutiuscula, Nitzschia palea, Amphora coffeaeformis and Entomoneis paludosa). Zn-supplementation reduced the maximum cell density. A linear relationship was found between the evolution of gross photosynthesis and total chlorophyll content. The Zn treatment decreased the electron transport rate except in A. coffeaeformis and in E. paludosa at high irradiance. A linear relationship was found between the efficiency of light to evolve oxygen and the size of the light-harvesting antenna. The external carbonic anhydrase activity was stimulated in Zn-supplemented E. paludosa but was not correlated with an increase of photosynthesis. The total activity of the antioxidant enzymes did not display any clear increase except in ascorbate peroxidase activity in N. palea. The phytochelatin synthase gene was identified in the four diatoms, but its expression was only revealed in N. palea, without a clear difference between control and Zn-supplemented cells. Among the four species, A. paludosa was the most sensitive and A. coffeaeformis, the most tolerant. A. acutiuscula seemed to be under metal starvation, whereas, to survive, only N. palea developed several stress responses. |
| format | Article |
| id | doaj-art-ece74b18e5564d3fb28c5a2076e06b3f |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ece74b18e5564d3fb28c5a2076e06b3f2025-02-03T01:03:51ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/982957982957Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine DiatomsThi Le Nhung Nguyen-Deroche0Aurore Caruso1Thi Trung Le2Trang Viet Bui3Benoît Schoefs4Gérard Tremblin5Annick Morant-Manceau6Mer, Molécules, Santé, EA 2160, LUNAM Université, Faculté des Sciences et Techniques, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans cedex 9, FranceMer, Molécules, Santé, EA 2160, LUNAM Université, Faculté des Sciences et Techniques, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans cedex 9, FranceLaboratory of Plant Physiology, Department of Biology, University of Education of Ho Chi Minh City, 5th District, 280 An Duong Vuong, Ho Chi Minh City, VietnamPlant Physiology Department, Faculty of Biology, University of Natural Sciences, 227 Nguyen Van Cu, 5th District, Ho Chi Minh City, VietnamMer, Molécules, Santé, EA 2160, LUNAM Université, Faculté des Sciences et Techniques, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans cedex 9, FranceMer, Molécules, Santé, EA 2160, LUNAM Université, Faculté des Sciences et Techniques, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans cedex 9, FranceMer, Molécules, Santé, EA 2160, LUNAM Université, Faculté des Sciences et Techniques, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans cedex 9, FranceZinc-supplementation (20 μM) effects on growth, photosynthesis, antioxidant enzyme activities (superoxide dismutase, ascorbate peroxidase, catalase), and the expression of phytochelatin synthase gene were investigated in four marine diatoms (Amphora acutiuscula, Nitzschia palea, Amphora coffeaeformis and Entomoneis paludosa). Zn-supplementation reduced the maximum cell density. A linear relationship was found between the evolution of gross photosynthesis and total chlorophyll content. The Zn treatment decreased the electron transport rate except in A. coffeaeformis and in E. paludosa at high irradiance. A linear relationship was found between the efficiency of light to evolve oxygen and the size of the light-harvesting antenna. The external carbonic anhydrase activity was stimulated in Zn-supplemented E. paludosa but was not correlated with an increase of photosynthesis. The total activity of the antioxidant enzymes did not display any clear increase except in ascorbate peroxidase activity in N. palea. The phytochelatin synthase gene was identified in the four diatoms, but its expression was only revealed in N. palea, without a clear difference between control and Zn-supplemented cells. Among the four species, A. paludosa was the most sensitive and A. coffeaeformis, the most tolerant. A. acutiuscula seemed to be under metal starvation, whereas, to survive, only N. palea developed several stress responses.http://dx.doi.org/10.1100/2012/982957 |
| spellingShingle | Thi Le Nhung Nguyen-Deroche Aurore Caruso Thi Trung Le Trang Viet Bui Benoît Schoefs Gérard Tremblin Annick Morant-Manceau Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms The Scientific World Journal |
| title | Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms |
| title_full | Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms |
| title_fullStr | Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms |
| title_full_unstemmed | Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms |
| title_short | Zinc Affects Differently Growth, Photosynthesis, Antioxidant Enzyme Activities and Phytochelatin Synthase Expression of Four Marine Diatoms |
| title_sort | zinc affects differently growth photosynthesis antioxidant enzyme activities and phytochelatin synthase expression of four marine diatoms |
| url | http://dx.doi.org/10.1100/2012/982957 |
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