Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway
Soil alkalization is a major challenge for global crop production. This study reveals a novel defense mechanism in rice seedlings against alkaline stress, involving methyl jasmonate (MeJA) and auxin pathways. Under alkaline stress (15 mM Na2CO3), rice seedlings exhibited elevated levels of endogenou...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X24002653 |
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| author | Chun-Lan Chen Di Wu Qian-Kun Li Xiao-hu Liu Xu-Guang Niu Guo-Xian Zhang Yong-Yong Zhang Hui Zhang Chang-Jie Jiang |
| author_facet | Chun-Lan Chen Di Wu Qian-Kun Li Xiao-hu Liu Xu-Guang Niu Guo-Xian Zhang Yong-Yong Zhang Hui Zhang Chang-Jie Jiang |
| author_sort | Chun-Lan Chen |
| collection | DOAJ |
| description | Soil alkalization is a major challenge for global crop production. This study reveals a novel defense mechanism in rice seedlings against alkaline stress, involving methyl jasmonate (MeJA) and auxin pathways. Under alkaline stress (15 mM Na2CO3), rice seedlings exhibited elevated levels of endogenous MeJA and upregulation of JA-responsive genes. Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress. This treatment also upregulated genes associated with cell death suppression (OsBI1) and stress tolerance (OsJRL, OsNAC). Notably, MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots, and upregulated genes involved in IAA synthesis (OASA1, OASA2) and auxin signaling (Aux/IAA, ARFs). Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress and diminished the protective effect of MeJA. These results highlight the crucial role of MeJA-induced activation of auxin pathway in enhancing rice tolerance to alkaline stress, and provide valuable insights for developing strategies to improve crop resilience in alkaline soils. |
| format | Article |
| id | doaj-art-ece4046dc5ca4123a44c295abbbdcf2a |
| institution | DOAJ |
| issn | 2667-064X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-ece4046dc5ca4123a44c295abbbdcf2a2025-08-20T02:52:27ZengElsevierPlant Stress2667-064X2024-12-011410061210.1016/j.stress.2024.100612Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathwayChun-Lan Chen0Di Wu1Qian-Kun Li2Xiao-hu Liu3Xu-Guang Niu4Guo-Xian Zhang5Yong-Yong Zhang6Hui Zhang7Chang-Jie Jiang8College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, ChinaCollege of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, China; Pratacultural College, Inner Mongolia University for Nationalities, Tongliao 028000, Innei Mongolia, ChinaCollege of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, ChinaCollege of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, ChinaCollege of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, ChinaCollege of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, ChinaCollege of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, ChinaCollege of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, China; Corresponding authors.Rice Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China; Corresponding authors.Soil alkalization is a major challenge for global crop production. This study reveals a novel defense mechanism in rice seedlings against alkaline stress, involving methyl jasmonate (MeJA) and auxin pathways. Under alkaline stress (15 mM Na2CO3), rice seedlings exhibited elevated levels of endogenous MeJA and upregulation of JA-responsive genes. Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress. This treatment also upregulated genes associated with cell death suppression (OsBI1) and stress tolerance (OsJRL, OsNAC). Notably, MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots, and upregulated genes involved in IAA synthesis (OASA1, OASA2) and auxin signaling (Aux/IAA, ARFs). Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress and diminished the protective effect of MeJA. These results highlight the crucial role of MeJA-induced activation of auxin pathway in enhancing rice tolerance to alkaline stress, and provide valuable insights for developing strategies to improve crop resilience in alkaline soils.http://www.sciencedirect.com/science/article/pii/S2667064X24002653Rice (Oryza sativa L.)Alkaline stressMethyl jasmonate (MeJA)AuxinPhytohormoneCrosstalk |
| spellingShingle | Chun-Lan Chen Di Wu Qian-Kun Li Xiao-hu Liu Xu-Guang Niu Guo-Xian Zhang Yong-Yong Zhang Hui Zhang Chang-Jie Jiang Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway Plant Stress Rice (Oryza sativa L.) Alkaline stress Methyl jasmonate (MeJA) Auxin Phytohormone Crosstalk |
| title | Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway |
| title_full | Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway |
| title_fullStr | Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway |
| title_full_unstemmed | Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway |
| title_short | Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway |
| title_sort | methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway |
| topic | Rice (Oryza sativa L.) Alkaline stress Methyl jasmonate (MeJA) Auxin Phytohormone Crosstalk |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X24002653 |
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