Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells
This study aimed to investigate the effect of catalpol on the inflammatory response of murine microglial cell line (BV2 cells) induced by lipopolysaccharide. Cell proliferation activity was detected by CCK-8 assay. The morphology of BV2 was observed by an optical microscope. The inflammatory factor...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2024-12-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/76556 |
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| _version_ | 1832571138320891904 |
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| author | Sijie Liu Jiayi Zhu Hao Huang Jiaxiang Xu Biao Zhang Yi Luo |
| author_facet | Sijie Liu Jiayi Zhu Hao Huang Jiaxiang Xu Biao Zhang Yi Luo |
| author_sort | Sijie Liu |
| collection | DOAJ |
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This study aimed to investigate the effect of catalpol on the inflammatory response of murine microglial cell line (BV2 cells) induced by lipopolysaccharide. Cell proliferation activity was detected by CCK-8 assay. The morphology of BV2 was observed by an optical microscope. The inflammatory factors interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) were detected by enzyme‐linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) were detected by flow cytometry. Induced nitric oxide synthase (iNOS) level was detected by immunofluorescence. The results showed that 5 μg/mL catalpol did not effect the proliferation of BV2 cells, while 10 μg/mL catalpol significantly decreased the viability of BV2 cells. Then the experiment was carried out with 5 μg/mL catalpol. Catalpol can improve the morphology of lipopolysaccharide-induced BV2 cells, decrease the level of inflammatory factors, and reduce the production of iNOS and ROS. Therefore, catalpol can inhibit the lipopolysaccharide-induced activation of BV2 cells and has anti-inflammatory effects.
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| format | Article |
| id | doaj-art-ec8014df212048f9936ff0b16a3048a6 |
| institution | Kabale University |
| issn | 1991-0088 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj-art-ec8014df212048f9936ff0b16a3048a62025-02-02T13:09:57ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882024-12-0119310.3329/bjp.v19i3.76556Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cellsSijie Liu0https://orcid.org/0009-0003-9070-5097Jiayi Zhu1https://orcid.org/0009-0003-0463-9442Hao Huang2https://orcid.org/0009-0002-7438-4011Jiaxiang Xu3https://orcid.org/0009-0007-9576-8416Biao Zhang4https://orcid.org/0000-0002-0128-6149Yi Luo5Department of Neurology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Neurology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Neurology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Neurology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Central Laboratory, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Neurology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China. This study aimed to investigate the effect of catalpol on the inflammatory response of murine microglial cell line (BV2 cells) induced by lipopolysaccharide. Cell proliferation activity was detected by CCK-8 assay. The morphology of BV2 was observed by an optical microscope. The inflammatory factors interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) were detected by enzyme‐linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) were detected by flow cytometry. Induced nitric oxide synthase (iNOS) level was detected by immunofluorescence. The results showed that 5 μg/mL catalpol did not effect the proliferation of BV2 cells, while 10 μg/mL catalpol significantly decreased the viability of BV2 cells. Then the experiment was carried out with 5 μg/mL catalpol. Catalpol can improve the morphology of lipopolysaccharide-induced BV2 cells, decrease the level of inflammatory factors, and reduce the production of iNOS and ROS. Therefore, catalpol can inhibit the lipopolysaccharide-induced activation of BV2 cells and has anti-inflammatory effects. https://www.banglajol.info/index.php/BJP/article/view/76556BV2 cellCatalpol Immunofluorescence detectionInflammationLipopolysaccharideMicroglia |
| spellingShingle | Sijie Liu Jiayi Zhu Hao Huang Jiaxiang Xu Biao Zhang Yi Luo Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells Bangladesh Journal of Pharmacology BV2 cell Catalpol Immunofluorescence detection Inflammation Lipopolysaccharide Microglia |
| title | Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells |
| title_full | Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells |
| title_fullStr | Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells |
| title_full_unstemmed | Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells |
| title_short | Catalpol alleviates the lipopolysaccharide-induced inflammatory response of BV2 cells |
| title_sort | catalpol alleviates the lipopolysaccharide induced inflammatory response of bv2 cells |
| topic | BV2 cell Catalpol Immunofluorescence detection Inflammation Lipopolysaccharide Microglia |
| url | https://www.banglajol.info/index.php/BJP/article/view/76556 |
| work_keys_str_mv | AT sijieliu catalpolalleviatesthelipopolysaccharideinducedinflammatoryresponseofbv2cells AT jiayizhu catalpolalleviatesthelipopolysaccharideinducedinflammatoryresponseofbv2cells AT haohuang catalpolalleviatesthelipopolysaccharideinducedinflammatoryresponseofbv2cells AT jiaxiangxu catalpolalleviatesthelipopolysaccharideinducedinflammatoryresponseofbv2cells AT biaozhang catalpolalleviatesthelipopolysaccharideinducedinflammatoryresponseofbv2cells AT yiluo catalpolalleviatesthelipopolysaccharideinducedinflammatoryresponseofbv2cells |