Tannic acid elicits differential gene regulation in prostate cancer apoptosis
Prostate cancer is a significant global health concern that requires innovative therapeutic investigations. Here, the potential anticancer properties of tannic acid were evaluated by examining its effects on apoptosis in prostate cancer cell lines. PC-3 and LnCaP prostate adeno carcinoma cells, alon...
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| Format: | Article |
| Language: | English |
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2024-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2024-0020 |
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| author | Kandir Sinan Karakurt Sevtap Gökçek-Saraç Çiğdem Karakurt Serdar |
| author_facet | Kandir Sinan Karakurt Sevtap Gökçek-Saraç Çiğdem Karakurt Serdar |
| author_sort | Kandir Sinan |
| collection | DOAJ |
| description | Prostate cancer is a significant global health concern that requires innovative therapeutic investigations. Here, the potential anticancer properties of tannic acid were evaluated by examining its effects on apoptosis in prostate cancer cell lines. PC-3 and LnCaP prostate adeno carcinoma cells, along with PNT1A prostate control cells, were cultured and divided into untreated and tannic acid-treated groups. Cell proliferation, cytotoxicity, and effects of tannic acid on the cell death mechanism were evaluated. mRNA expression levels of 84 genes were explored in cells following tannic acid treatment. Notably, tannic acid-induced down-regulation of several pro-survival genes, including ATM, BCL2, BCL2A1, BIK, BIRC2, BIRC3, BRE, CASP3, CASP6, CASP8, CHEK2, CRADD, PPIA, RPA3, TNFSF18, TRAF1, TRAF2, TRAF4, and TRAF5 in both cell lines. Moreover, tannic acid treatment led to the up-regulation of various pro-apoptotic genes, such as BCL10, BIRC3, BNIP3, CASP1, CASP5, CD40, CIDEB, DAPK2, FASLG, GADD45A, MYD88, RPA 3, TNFRSF10D, TNFRSF17, TNFRSF8, TNFSF13B, TNFSF4, TNFSF7, TNFSF8, TNFSF9, TP53, TRAF1, and TRAF2 in both PC-3 and LnCap cells. These findings highlight tannic acid’s ability to induce apoptosis in prostate cancer cells through pro-apoptotic pathways. This study concludes that tannic acid selectively inhibits prostate cancer cell growth. |
| format | Article |
| id | doaj-art-ec710202e29a4708833d1799c9b685a7 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-ec710202e29a4708833d1799c9b685a72025-02-03T00:09:52ZengSciendoActa Pharmaceutica1846-95582024-09-0174353955010.2478/acph-2024-0020Tannic acid elicits differential gene regulation in prostate cancer apoptosisKandir Sinan0Karakurt Sevtap1Gökçek-Saraç Çiğdem2Karakurt Serdar3Faculty of Veterinary Medicine, Department of Physiology, Çukurova University, 01920Ceyhan Adana, TurkiyeFaculty of Science Department of BiochemistrySelçuk University42250Konya, TurkiyeFaculty of Engineering Department of Biomedical Engineering, Akdeniz University, 07050AntalyaTurkiyeFaculty of Science Department of BiochemistrySelçuk University42250Konya, TurkiyeProstate cancer is a significant global health concern that requires innovative therapeutic investigations. Here, the potential anticancer properties of tannic acid were evaluated by examining its effects on apoptosis in prostate cancer cell lines. PC-3 and LnCaP prostate adeno carcinoma cells, along with PNT1A prostate control cells, were cultured and divided into untreated and tannic acid-treated groups. Cell proliferation, cytotoxicity, and effects of tannic acid on the cell death mechanism were evaluated. mRNA expression levels of 84 genes were explored in cells following tannic acid treatment. Notably, tannic acid-induced down-regulation of several pro-survival genes, including ATM, BCL2, BCL2A1, BIK, BIRC2, BIRC3, BRE, CASP3, CASP6, CASP8, CHEK2, CRADD, PPIA, RPA3, TNFSF18, TRAF1, TRAF2, TRAF4, and TRAF5 in both cell lines. Moreover, tannic acid treatment led to the up-regulation of various pro-apoptotic genes, such as BCL10, BIRC3, BNIP3, CASP1, CASP5, CD40, CIDEB, DAPK2, FASLG, GADD45A, MYD88, RPA 3, TNFRSF10D, TNFRSF17, TNFRSF8, TNFSF13B, TNFSF4, TNFSF7, TNFSF8, TNFSF9, TP53, TRAF1, and TRAF2 in both PC-3 and LnCap cells. These findings highlight tannic acid’s ability to induce apoptosis in prostate cancer cells through pro-apoptotic pathways. This study concludes that tannic acid selectively inhibits prostate cancer cell growth.https://doi.org/10.2478/acph-2024-0020tannic acidprostate cancerapoptosisgene regulationselective cytotoxicity |
| spellingShingle | Kandir Sinan Karakurt Sevtap Gökçek-Saraç Çiğdem Karakurt Serdar Tannic acid elicits differential gene regulation in prostate cancer apoptosis Acta Pharmaceutica tannic acid prostate cancer apoptosis gene regulation selective cytotoxicity |
| title | Tannic acid elicits differential gene regulation in prostate cancer apoptosis |
| title_full | Tannic acid elicits differential gene regulation in prostate cancer apoptosis |
| title_fullStr | Tannic acid elicits differential gene regulation in prostate cancer apoptosis |
| title_full_unstemmed | Tannic acid elicits differential gene regulation in prostate cancer apoptosis |
| title_short | Tannic acid elicits differential gene regulation in prostate cancer apoptosis |
| title_sort | tannic acid elicits differential gene regulation in prostate cancer apoptosis |
| topic | tannic acid prostate cancer apoptosis gene regulation selective cytotoxicity |
| url | https://doi.org/10.2478/acph-2024-0020 |
| work_keys_str_mv | AT kandirsinan tannicacidelicitsdifferentialgeneregulationinprostatecancerapoptosis AT karakurtsevtap tannicacidelicitsdifferentialgeneregulationinprostatecancerapoptosis AT gokceksaraccigdem tannicacidelicitsdifferentialgeneregulationinprostatecancerapoptosis AT karakurtserdar tannicacidelicitsdifferentialgeneregulationinprostatecancerapoptosis |