Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts
Reconstitution of the hematopoietic system during immune responses and immunological and neoplastic diseases or upon transplantation depends on the emergent differentiation of hematopoietic stem/progenitor cells within the bone marrow. Although in the last decade the use of dialyzable leukocyte extr...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4097642 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555820508774400 |
|---|---|
| author | Dalia Ramírez-Ramírez Eduardo Vadillo Lourdes Andrea Arriaga-Pizano Héctor Mayani Sergio Estrada-Parra Marco Antonio Velasco-Velázquez Sonia Mayra Pérez-Tapia Rosana Pelayo |
| author_facet | Dalia Ramírez-Ramírez Eduardo Vadillo Lourdes Andrea Arriaga-Pizano Héctor Mayani Sergio Estrada-Parra Marco Antonio Velasco-Velázquez Sonia Mayra Pérez-Tapia Rosana Pelayo |
| author_sort | Dalia Ramírez-Ramírez |
| collection | DOAJ |
| description | Reconstitution of the hematopoietic system during immune responses and immunological and neoplastic diseases or upon transplantation depends on the emergent differentiation of hematopoietic stem/progenitor cells within the bone marrow. Although in the last decade the use of dialyzable leukocyte extracts (DLE) as supportive therapy in both infectious and malignant settings has increased, its activity on the earliest stages of human hematopoietic development remains poorly understood. Here, we have examined the ability of DLE to promote replenishment of functional lymphoid lineages from CD34+ cells. Our findings suggest that DLE increases their differentiation toward a conspicuous CD56+CD16+CD11c+ NK-like cell population endowed with properties such as IFNy production, tumor cell cytotoxicity, and the capability of inducing γδ T lymphocyte proliferation. Of note, long-term coculture controlled systems showed the bystander effect of DLE-stromal cells by providing NK progenitors with signals to overproduce this cell subset. Thus, by direct effect on progenitor cells and through activation and remodeling of the supporting hematopoietic microenvironment, DLE may contribute a robust innate immune response by promoting the emerging lymphopoiesis of functional CD11c+ NK cells in a partially TLR-related manner. Unraveling the identity and mechanisms of the involved DLE components may be fundamental to advance the NK cell-based therapy field. |
| format | Article |
| id | doaj-art-ec6408d8f1ef4d8b92ef5fcc6e05dfb7 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-ec6408d8f1ef4d8b92ef5fcc6e05dfb72025-02-03T05:47:13ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/40976424097642Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte ExtractsDalia Ramírez-Ramírez0Eduardo Vadillo1Lourdes Andrea Arriaga-Pizano2Héctor Mayani3Sergio Estrada-Parra4Marco Antonio Velasco-Velázquez5Sonia Mayra Pérez-Tapia6Rosana Pelayo7Oncology Research Unit, Oncology Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, MexicoOncology Research Unit, Oncology Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, MexicoImmunochemistry Research Unit, Medical Specialties Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, MexicoOncology Research Unit, Oncology Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, MexicoDepartment of Immunology, National School of Biological Sciences (ENCB), National Polytechnic Institute (IPN), Carpio y Plan de Ayala s/n, Colonia Santo Tomás, 11340 Mexico City, MexicoDepartment of Pharmacology, School of Medicine, National Autonomous University of Mexico, Ciudad Universitaria, 04510 Mexico City, MexicoDepartment of Immunology, National School of Biological Sciences (ENCB), National Polytechnic Institute (IPN), Carpio y Plan de Ayala s/n, Colonia Santo Tomás, 11340 Mexico City, MexicoOncology Research Unit, Oncology Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, MexicoReconstitution of the hematopoietic system during immune responses and immunological and neoplastic diseases or upon transplantation depends on the emergent differentiation of hematopoietic stem/progenitor cells within the bone marrow. Although in the last decade the use of dialyzable leukocyte extracts (DLE) as supportive therapy in both infectious and malignant settings has increased, its activity on the earliest stages of human hematopoietic development remains poorly understood. Here, we have examined the ability of DLE to promote replenishment of functional lymphoid lineages from CD34+ cells. Our findings suggest that DLE increases their differentiation toward a conspicuous CD56+CD16+CD11c+ NK-like cell population endowed with properties such as IFNy production, tumor cell cytotoxicity, and the capability of inducing γδ T lymphocyte proliferation. Of note, long-term coculture controlled systems showed the bystander effect of DLE-stromal cells by providing NK progenitors with signals to overproduce this cell subset. Thus, by direct effect on progenitor cells and through activation and remodeling of the supporting hematopoietic microenvironment, DLE may contribute a robust innate immune response by promoting the emerging lymphopoiesis of functional CD11c+ NK cells in a partially TLR-related manner. Unraveling the identity and mechanisms of the involved DLE components may be fundamental to advance the NK cell-based therapy field.http://dx.doi.org/10.1155/2016/4097642 |
| spellingShingle | Dalia Ramírez-Ramírez Eduardo Vadillo Lourdes Andrea Arriaga-Pizano Héctor Mayani Sergio Estrada-Parra Marco Antonio Velasco-Velázquez Sonia Mayra Pérez-Tapia Rosana Pelayo Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts Journal of Immunology Research |
| title | Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts |
| title_full | Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts |
| title_fullStr | Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts |
| title_full_unstemmed | Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts |
| title_short | Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts |
| title_sort | early differentiation of human cd11c nk cells with γδ t cell activation properties is promoted by dialyzable leukocyte extracts |
| url | http://dx.doi.org/10.1155/2016/4097642 |
| work_keys_str_mv | AT daliaramirezramirez earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts AT eduardovadillo earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts AT lourdesandreaarriagapizano earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts AT hectormayani earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts AT sergioestradaparra earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts AT marcoantoniovelascovelazquez earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts AT soniamayrapereztapia earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts AT rosanapelayo earlydifferentiationofhumancd11cnkcellswithgdtcellactivationpropertiesispromotedbydialyzableleukocyteextracts |