Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in Taiwan
Background/purpose(s): Dengue virus (DENV) is one of the most troublesome mosquito-borne infectious viruses in tropical and subtropical zones. People with secondary/multiple DENV infections are at an increased risk of developing severe dengue. Both monocytes and T cells are known to play important r...
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Elsevier
2025-02-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118224002123 |
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| author | Sheng-Hsuan Wang Yun-Erh Chuang Sia-Seng Tan Tzu-Chuan Ho Oscar Guey Chuen Perng Po-Lin Chen |
| author_facet | Sheng-Hsuan Wang Yun-Erh Chuang Sia-Seng Tan Tzu-Chuan Ho Oscar Guey Chuen Perng Po-Lin Chen |
| author_sort | Sheng-Hsuan Wang |
| collection | DOAJ |
| description | Background/purpose(s): Dengue virus (DENV) is one of the most troublesome mosquito-borne infectious viruses in tropical and subtropical zones. People with secondary/multiple DENV infections are at an increased risk of developing severe dengue. Both monocytes and T cells are known to play important roles in the immune response against DENV. However, the function of monocytes and T cells in individuals with potentially multiple exposures to DENV is rarely reported. Method: In the present study, we performed a functional analysis of monocytes and T cells from people with previous DENV infection and DENV-naïve people that stimulated with DENV2 ex vivo. Results: Our preliminary analysis indicated that the response of monocytes and T cells to DENV2 restimulation was comparable between DENV-exposed and DENV-naïve individuals. Furthermore, the cytokine expression profiles in monocytes from both naïve individuals and previously DENV-exposed subjects were similar after DENV2 stimulation. In addition, it was observed that the function of T cells was also equivalent when monocytes were present as antigen-presenting cells for dengue antigen, NS3, in terms of cell proliferation, interferon-gamma (IFNγ) secretion, and memory response. Conclusions: Based on the results, it was observed that previously DENV-exposed monocytes and T cells seemed to be anergic during DENV reinfection. However, whether the impaired response of monocytes and T cells against DENV in people with a history of previous DENV infection leads to severe dengue upon secondary infection in endemic areas requires further investigation. |
| format | Article |
| id | doaj-art-ec62f998e24640c8821e54e58aeab4ba |
| institution | Kabale University |
| issn | 1684-1182 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj-art-ec62f998e24640c8821e54e58aeab4ba2025-02-06T05:11:21ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822025-02-015811726Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in TaiwanSheng-Hsuan Wang0Yun-Erh Chuang1Sia-Seng Tan2Tzu-Chuan Ho3Oscar Guey Chuen Perng4Po-Lin Chen5Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanInstitute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, TaiwanInstitute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Infection Control Center, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Corresponding author. Department of Internal Medicine, National Cheng Kung University Hospital, No. 138, Sheng Li Road, 704, Tainan, Taiwan.Background/purpose(s): Dengue virus (DENV) is one of the most troublesome mosquito-borne infectious viruses in tropical and subtropical zones. People with secondary/multiple DENV infections are at an increased risk of developing severe dengue. Both monocytes and T cells are known to play important roles in the immune response against DENV. However, the function of monocytes and T cells in individuals with potentially multiple exposures to DENV is rarely reported. Method: In the present study, we performed a functional analysis of monocytes and T cells from people with previous DENV infection and DENV-naïve people that stimulated with DENV2 ex vivo. Results: Our preliminary analysis indicated that the response of monocytes and T cells to DENV2 restimulation was comparable between DENV-exposed and DENV-naïve individuals. Furthermore, the cytokine expression profiles in monocytes from both naïve individuals and previously DENV-exposed subjects were similar after DENV2 stimulation. In addition, it was observed that the function of T cells was also equivalent when monocytes were present as antigen-presenting cells for dengue antigen, NS3, in terms of cell proliferation, interferon-gamma (IFNγ) secretion, and memory response. Conclusions: Based on the results, it was observed that previously DENV-exposed monocytes and T cells seemed to be anergic during DENV reinfection. However, whether the impaired response of monocytes and T cells against DENV in people with a history of previous DENV infection leads to severe dengue upon secondary infection in endemic areas requires further investigation.http://www.sciencedirect.com/science/article/pii/S1684118224002123Dengue virusMonocytesT cellsPreviously DENV-Exposed subjectsInterferon-gamma |
| spellingShingle | Sheng-Hsuan Wang Yun-Erh Chuang Sia-Seng Tan Tzu-Chuan Ho Oscar Guey Chuen Perng Po-Lin Chen Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in Taiwan Journal of Microbiology, Immunology and Infection Dengue virus Monocytes T cells Previously DENV-Exposed subjects Interferon-gamma |
| title | Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in Taiwan |
| title_full | Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in Taiwan |
| title_fullStr | Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in Taiwan |
| title_full_unstemmed | Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in Taiwan |
| title_short | Comparative monocyte and T cell responses in DENV-exposed subjects from South-East Asia and DENV-naïve residents in Taiwan |
| title_sort | comparative monocyte and t cell responses in denv exposed subjects from south east asia and denv naive residents in taiwan |
| topic | Dengue virus Monocytes T cells Previously DENV-Exposed subjects Interferon-gamma |
| url | http://www.sciencedirect.com/science/article/pii/S1684118224002123 |
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