Transplantation of Amniotic Membrane to the Subretinal Space in Pigs
Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch’s membrane (BM) and served as a control group. 15 pigs additionally had AM...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/716968 |
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| _version_ | 1832552887131045888 |
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| author | Jens Folke Kiilgaard Erik Scherfig Jan Ulrik Prause Morten la Cour |
| author_facet | Jens Folke Kiilgaard Erik Scherfig Jan Ulrik Prause Morten la Cour |
| author_sort | Jens Folke Kiilgaard |
| collection | DOAJ |
| description | Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch’s membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space. Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P<0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE. Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE. |
| format | Article |
| id | doaj-art-ec60840a22024e5c96de252fa320098a |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ec60840a22024e5c96de252fa320098a2025-02-03T05:57:37ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/716968716968Transplantation of Amniotic Membrane to the Subretinal Space in PigsJens Folke Kiilgaard0Erik Scherfig1Jan Ulrik Prause2Morten la Cour3Department of Ophthalmology, Glostrup Hospital, Copenhagen University Hospital, Nordre Ringvej 57, 2600 Glostrup, DenmarkEye Pathology Institute, Teilumbygningen, University of Copenhagen, 2100 Copenhagen, DenmarkEye Pathology Institute, Teilumbygningen, University of Copenhagen, 2100 Copenhagen, DenmarkDepartment of Ophthalmology, Glostrup Hospital, Copenhagen University Hospital, Nordre Ringvej 57, 2600 Glostrup, DenmarkPurpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch’s membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space. Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P<0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE. Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE.http://dx.doi.org/10.1155/2012/716968 |
| spellingShingle | Jens Folke Kiilgaard Erik Scherfig Jan Ulrik Prause Morten la Cour Transplantation of Amniotic Membrane to the Subretinal Space in Pigs Stem Cells International |
| title | Transplantation of Amniotic Membrane to the Subretinal Space in Pigs |
| title_full | Transplantation of Amniotic Membrane to the Subretinal Space in Pigs |
| title_fullStr | Transplantation of Amniotic Membrane to the Subretinal Space in Pigs |
| title_full_unstemmed | Transplantation of Amniotic Membrane to the Subretinal Space in Pigs |
| title_short | Transplantation of Amniotic Membrane to the Subretinal Space in Pigs |
| title_sort | transplantation of amniotic membrane to the subretinal space in pigs |
| url | http://dx.doi.org/10.1155/2012/716968 |
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