Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines
The secretion of extracellular membrane vesicles (EMVs) is a common phenomenon that occurs in archaea, bacteria, and mammalian cells. The EMVs of bacteria play important roles in their virulence, biogenesis mechanisms, and host cell interactions. Bacterial EMVs have recently become the focus of atte...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/7931982 |
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| author | Chi-Won Choi Edmond Changkyun Park Sung Ho Yun Sang-Yeop Lee Seung Il Kim Gun-Hwa Kim |
| author_facet | Chi-Won Choi Edmond Changkyun Park Sung Ho Yun Sang-Yeop Lee Seung Il Kim Gun-Hwa Kim |
| author_sort | Chi-Won Choi |
| collection | DOAJ |
| description | The secretion of extracellular membrane vesicles (EMVs) is a common phenomenon that occurs in archaea, bacteria, and mammalian cells. The EMVs of bacteria play important roles in their virulence, biogenesis mechanisms, and host cell interactions. Bacterial EMVs have recently become the focus of attention because of their potential as highly effective vaccines that cause few side effects. Here, we isolated the EMVs of Streptococcus pneumoniae and examined their potential as new vaccine candidates. Although the S. pneumoniae bacteria were highly pathogenic in a mouse model, the EMVs purified from these bacteria showed low pathological activity both in cell culture and in mice. When mice were injected intraperitoneally with S. pneumoniae EMVs and then challenged, they were protected from both the homologous strain and another pathogenic serotype of S. pneumoniae. We also identified a number of proteins that may have immunogenic activity and may be responsible for the immune responses by the hosts. These results suggest that S. pneumoniae EMVs or their individual immunogenic antigens may be useful as new vaccine agents. |
| format | Article |
| id | doaj-art-ec050f11a624402aa24eb4a9a955d5ad |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-ec050f11a624402aa24eb4a9a955d5ad2025-02-03T05:54:09ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/79319827931982Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial VaccinesChi-Won Choi0Edmond Changkyun Park1Sung Ho Yun2Sang-Yeop Lee3Seung Il Kim4Gun-Hwa Kim5Division of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of KoreaDivision of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of KoreaDivision of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of KoreaDivision of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of KoreaDivision of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of KoreaDivision of Bioconvergence Analysis, Korea Basic Science Institute, Daejeon, Republic of KoreaThe secretion of extracellular membrane vesicles (EMVs) is a common phenomenon that occurs in archaea, bacteria, and mammalian cells. The EMVs of bacteria play important roles in their virulence, biogenesis mechanisms, and host cell interactions. Bacterial EMVs have recently become the focus of attention because of their potential as highly effective vaccines that cause few side effects. Here, we isolated the EMVs of Streptococcus pneumoniae and examined their potential as new vaccine candidates. Although the S. pneumoniae bacteria were highly pathogenic in a mouse model, the EMVs purified from these bacteria showed low pathological activity both in cell culture and in mice. When mice were injected intraperitoneally with S. pneumoniae EMVs and then challenged, they were protected from both the homologous strain and another pathogenic serotype of S. pneumoniae. We also identified a number of proteins that may have immunogenic activity and may be responsible for the immune responses by the hosts. These results suggest that S. pneumoniae EMVs or their individual immunogenic antigens may be useful as new vaccine agents.http://dx.doi.org/10.1155/2017/7931982 |
| spellingShingle | Chi-Won Choi Edmond Changkyun Park Sung Ho Yun Sang-Yeop Lee Seung Il Kim Gun-Hwa Kim Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines Journal of Immunology Research |
| title | Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines |
| title_full | Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines |
| title_fullStr | Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines |
| title_full_unstemmed | Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines |
| title_short | Potential Usefulness of Streptococcus pneumoniae Extracellular Membrane Vesicles as Antibacterial Vaccines |
| title_sort | potential usefulness of streptococcus pneumoniae extracellular membrane vesicles as antibacterial vaccines |
| url | http://dx.doi.org/10.1155/2017/7931982 |
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