Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering
Hydrogel scaffolds play a crucial role in tissue engineering; however, traditional bulk hydrogel scaffolds (BHS) often suffer from insufficiently sized pores (nanoscales), impeding cellular infiltration, development, and expansion. This limitation affects oxygen and nutrient exchange efficiency, in...
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Elsevier
2025-08-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425005769 |
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| author | Yaqi Guo Shan Mou Litao Suo Yingqian Zhou Shuang Wu Xinfang Xie Di Sun Bingqian Wang Zhenxing Wang Raymund E. Horch Jie Yang Jiaming Sun |
| author_facet | Yaqi Guo Shan Mou Litao Suo Yingqian Zhou Shuang Wu Xinfang Xie Di Sun Bingqian Wang Zhenxing Wang Raymund E. Horch Jie Yang Jiaming Sun |
| author_sort | Yaqi Guo |
| collection | DOAJ |
| description | Hydrogel scaffolds play a crucial role in tissue engineering; however, traditional bulk hydrogel scaffolds (BHS) often suffer from insufficiently sized pores (nanoscales), impeding cellular infiltration, development, and expansion. This limitation affects oxygen and nutrient exchange efficiency, in which case it relies extensively on liquid permeation and bulk hydrogels swelling. In contrast, hydrogel microparticles (HMPs) have proven to be both printable and injectable, allowing the development of modular thick constructs with interconnected pores. This study introduces a novel method of fabricating porous granular hydrogel scaffolds (GHS) by printing thermo-crosslinked gelatin methacryloyl (GelMA) HMPs granular hydrogels before chemical crosslinking (dual-crosslinking). The scaffolds exhibit an average pore fraction ranging from 14 % to 23 % and an average pore size varying from 4923 μm2 to 8185 μm2 (with equivalent circular diameter of 80–102 μm). In vitro experiments demonstrated the effective infiltration, adhesion, proliferation, and adipogenic differentiation of human adipose-derived stem cells (hADSCs) within the scaffold pores. Additionally, in vivo observations confirmed the presence of differentiated adipose cells within the central pores after 4 weeks. These results collectively suggest the proposed microspheres printing technique holds significant promise for fabricating microporous scaffolds and further applications in tissue engineering. |
| format | Article |
| id | doaj-art-ebeebd3f6de04724a974de5dcbe3bb9e |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-ebeebd3f6de04724a974de5dcbe3bb9e2025-08-20T03:27:05ZengElsevierMaterials Today Bio2590-00642025-08-013310200610.1016/j.mtbio.2025.102006Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineeringYaqi Guo0Shan Mou1Litao Suo2Yingqian Zhou3Shuang Wu4Xinfang Xie5Di Sun6Bingqian Wang7Zhenxing Wang8Raymund E. Horch9Jie Yang10Jiaming Sun11Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China; Wuhan Clinical Research Center for Superficial Organ Reconstruction, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of ChinaDepartment of Plastic and Hand Surgery and Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital Erlangen, Friedrich Alexander University of Erlangen-Nürnberg, FAU, Schlossplatz 4, Erlangen, 91054, Bavaria, Germany; Corresponding author.Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China; Corresponding author.Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China; Wuhan Clinical Research Center for Superficial Organ Reconstruction, Wuhan, 430022, People's Republic of China; Corresponding author. Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.Hydrogel scaffolds play a crucial role in tissue engineering; however, traditional bulk hydrogel scaffolds (BHS) often suffer from insufficiently sized pores (nanoscales), impeding cellular infiltration, development, and expansion. This limitation affects oxygen and nutrient exchange efficiency, in which case it relies extensively on liquid permeation and bulk hydrogels swelling. In contrast, hydrogel microparticles (HMPs) have proven to be both printable and injectable, allowing the development of modular thick constructs with interconnected pores. This study introduces a novel method of fabricating porous granular hydrogel scaffolds (GHS) by printing thermo-crosslinked gelatin methacryloyl (GelMA) HMPs granular hydrogels before chemical crosslinking (dual-crosslinking). The scaffolds exhibit an average pore fraction ranging from 14 % to 23 % and an average pore size varying from 4923 μm2 to 8185 μm2 (with equivalent circular diameter of 80–102 μm). In vitro experiments demonstrated the effective infiltration, adhesion, proliferation, and adipogenic differentiation of human adipose-derived stem cells (hADSCs) within the scaffold pores. Additionally, in vivo observations confirmed the presence of differentiated adipose cells within the central pores after 4 weeks. These results collectively suggest the proposed microspheres printing technique holds significant promise for fabricating microporous scaffolds and further applications in tissue engineering.http://www.sciencedirect.com/science/article/pii/S2590006425005769Hydrogel microparticlesGranular hydrogel scaffoldsExtrusion printinghADSCsBreast tissue engineering |
| spellingShingle | Yaqi Guo Shan Mou Litao Suo Yingqian Zhou Shuang Wu Xinfang Xie Di Sun Bingqian Wang Zhenxing Wang Raymund E. Horch Jie Yang Jiaming Sun Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering Materials Today Bio Hydrogel microparticles Granular hydrogel scaffolds Extrusion printing hADSCs Breast tissue engineering |
| title | Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering |
| title_full | Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering |
| title_fullStr | Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering |
| title_full_unstemmed | Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering |
| title_short | Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering |
| title_sort | porous granular hydrogel scaffolds biofabricated from dual crosslinked hydrogel microparticles for breast tissue engineering |
| topic | Hydrogel microparticles Granular hydrogel scaffolds Extrusion printing hADSCs Breast tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425005769 |
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