Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock
Changes in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/496121 |
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| author | Nathalia Oda Amaral Lara Marques Naves Marcos Luiz Ferreira-Neto André Henrique Freiria-Oliveira Eduardo Colombari Daniel Alves Rosa Angela Adamski da Silva Reis Danielle Ianzer Carlos Henrique Xavier Gustavo Rodrigues Pedrino |
| author_facet | Nathalia Oda Amaral Lara Marques Naves Marcos Luiz Ferreira-Neto André Henrique Freiria-Oliveira Eduardo Colombari Daniel Alves Rosa Angela Adamski da Silva Reis Danielle Ianzer Carlos Henrique Xavier Gustavo Rodrigues Pedrino |
| author_sort | Nathalia Oda Amaral |
| collection | DOAJ |
| description | Changes in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline infusion (HSI) in rats submitted to hemorrhagic shock. The recordings of mean arterial pressure (MAP) and renal vascular conductance (RVC) were carried out on male Wistar rats (250–300 g). Hemorrhagic shock was induced by blood withdrawal over 20 min until the MAP values of approximately 60 mmHg were attained. The nanoinjection (100 nL) of GABAA agonist (Muscimol 4 mM; experimental group (EXP)) or isotonic saline (NaCl 150 mM; control (CONT)) into MnPO was performed 2 min prior to intravenous overload of sodium through HSI (3 M NaCl, 1.8 mL/kg, b.wt.). Hemorrhagic shock reduced the MAP in control (62±1.1 mmHg) and EXP (61±0.4 mmHg) equipotently. The inhibition of MnPO impaired MAP (CONT: 104±4.2 versus EXP: 60±6.2 mmHg) and RVC (CONT: 6.4±11.4 versus EXP: -53.5±10.0) recovery 10 min after HSI. The overall results in this study demonstrated, for the first time, that the MnPO plays an essential role in the HSI induced resuscitation during hypovolemic hemorrhagic shock. |
| format | Article |
| id | doaj-art-ebe7ecae9122419e98ed78818ba9edea |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ebe7ecae9122419e98ed78818ba9edea2025-02-03T01:25:59ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/496121496121Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic ShockNathalia Oda Amaral0Lara Marques Naves1Marcos Luiz Ferreira-Neto2André Henrique Freiria-Oliveira3Eduardo Colombari4Daniel Alves Rosa5Angela Adamski da Silva Reis6Danielle Ianzer7Carlos Henrique Xavier8Gustavo Rodrigues Pedrino9Center for Neuroscience and Cardiovascular Physiology, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilCenter for Neuroscience and Cardiovascular Physiology, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilFaculty of Physical Education, Federal University of Uberlândia, Rua Bejamin Constant 1286, 14801-903 Uberlândia, MG, BrazilCenter for Neuroscience and Cardiovascular Physiology, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilDepartment of Physiology and Pathology, São Paulo State University (UNESP), Rua Humaita 1680, 14801-903 Araraquara, SP, BrazilCenter for Neuroscience and Cardiovascular Physiology, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilDepartment of Biochemistry and Molecular Biology, Biological Sciences Institute, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilCenter for Neuroscience and Cardiovascular Physiology, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilCenter for Neuroscience and Cardiovascular Physiology, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilCenter for Neuroscience and Cardiovascular Physiology, Federal University of Goiás, Estrada do Campus s/n, 74690-900 Goiânia, GO, BrazilChanges in plasma osmolarity, through central and peripheral osmoreceptors, activate the median preoptic nucleus (MnPO) that modulates autonomic and neuroendocrine adjustments. The present study sought to determine the participation of MnPO in the cardiovascular recovery induced by hypertonic saline infusion (HSI) in rats submitted to hemorrhagic shock. The recordings of mean arterial pressure (MAP) and renal vascular conductance (RVC) were carried out on male Wistar rats (250–300 g). Hemorrhagic shock was induced by blood withdrawal over 20 min until the MAP values of approximately 60 mmHg were attained. The nanoinjection (100 nL) of GABAA agonist (Muscimol 4 mM; experimental group (EXP)) or isotonic saline (NaCl 150 mM; control (CONT)) into MnPO was performed 2 min prior to intravenous overload of sodium through HSI (3 M NaCl, 1.8 mL/kg, b.wt.). Hemorrhagic shock reduced the MAP in control (62±1.1 mmHg) and EXP (61±0.4 mmHg) equipotently. The inhibition of MnPO impaired MAP (CONT: 104±4.2 versus EXP: 60±6.2 mmHg) and RVC (CONT: 6.4±11.4 versus EXP: -53.5±10.0) recovery 10 min after HSI. The overall results in this study demonstrated, for the first time, that the MnPO plays an essential role in the HSI induced resuscitation during hypovolemic hemorrhagic shock.http://dx.doi.org/10.1155/2014/496121 |
| spellingShingle | Nathalia Oda Amaral Lara Marques Naves Marcos Luiz Ferreira-Neto André Henrique Freiria-Oliveira Eduardo Colombari Daniel Alves Rosa Angela Adamski da Silva Reis Danielle Ianzer Carlos Henrique Xavier Gustavo Rodrigues Pedrino Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock The Scientific World Journal |
| title | Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock |
| title_full | Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock |
| title_fullStr | Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock |
| title_full_unstemmed | Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock |
| title_short | Median Preoptic Nucleus Mediates the Cardiovascular Recovery Induced by Hypertonic Saline in Hemorrhagic Shock |
| title_sort | median preoptic nucleus mediates the cardiovascular recovery induced by hypertonic saline in hemorrhagic shock |
| url | http://dx.doi.org/10.1155/2014/496121 |
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