Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures
Summary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modif...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672500187X |
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| author | Stina Oredsson Atena Malakpour-Permlid Johan Zhu Tilo Weber |
| author_facet | Stina Oredsson Atena Malakpour-Permlid Johan Zhu Tilo Weber |
| author_sort | Stina Oredsson |
| collection | DOAJ |
| description | Summary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-eba82bdc1ca84a3ca4cc954d3dfd9cee |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-eba82bdc1ca84a3ca4cc954d3dfd9cee2025-08-20T03:52:24ZengElsevierSTAR Protocols2666-16672025-06-016210378110.1016/j.xpro.2025.103781Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension culturesStina Oredsson0Atena Malakpour-Permlid1Johan Zhu2Tilo Weber3Department of Biology, Lund University, 22362 Lund, Sweden; Corresponding authorCenter for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkClinical Microbiology and Infection Prevention and Control, Region Skåne, 22185 Lund, SwedenAnimal Welfare Academy of the German Animal Welfare Federation, 85579 Neubiberg, GermanySummary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672500187XCell cultureCell-based assaysCancer |
| spellingShingle | Stina Oredsson Atena Malakpour-Permlid Johan Zhu Tilo Weber Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures STAR Protocols Cell culture Cell-based assays Cancer |
| title | Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures |
| title_full | Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures |
| title_fullStr | Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures |
| title_full_unstemmed | Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures |
| title_short | Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures |
| title_sort | protocol for the use of oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures |
| topic | Cell culture Cell-based assays Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S266616672500187X |
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