Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell Line
Background. Chemotherapy, radiotherapy, and surgery are routine treatments of breast cancer. However, these methods could only improve the living survival. Nowadays the combined therapy including herbals such as crocin is to study for improving breast cancer treatment. The purpose of this study was...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | International Journal of Breast Cancer |
| Online Access: | http://dx.doi.org/10.1155/2015/139349 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545750163128320 |
|---|---|
| author | Faeze Vali Vahid Changizi Majid Safa |
| author_facet | Faeze Vali Vahid Changizi Majid Safa |
| author_sort | Faeze Vali |
| collection | DOAJ |
| description | Background. Chemotherapy, radiotherapy, and surgery are routine treatments of breast cancer. However, these methods could only improve the living survival. Nowadays the combined therapy including herbals such as crocin is to study for improving breast cancer treatment. The purpose of this study was to evaluate the effects of crocin, paclitaxel, and radiation on MCF-7 cell. Methods. To evaluate the effect of crocin, paclitaxel, and radiation on survival rate of MCF-7 cells MTT assay was done. To investigate the apoptotic effect of experimental groups PI-flow cytometry was used and expression of apoptotic proteins (caspase-7, caspase-9, PARP, and p53) was studied by western blot. Results. This study revealed that the combined therapy of 0.01µmol/mL paclitaxel and 2.5 mg/mL crocin after 48 h could cause IC50 for MCF-7 cell line. This study showed that the combined therapy of 2 Gy gamma radiation with crocin could rise apoptosis in MCF-7 cell line from 21% (related to using 2 Gy gamma radiation alone) to 46.6%. Conclusion. Crocin and paclitaxel and crocin and gamma radiation had synergistic effect on MCF-7 cell line to get more significant apoptosis. |
| format | Article |
| id | doaj-art-eb62ad0d9134430f824adde4cda6905a |
| institution | Kabale University |
| issn | 2090-3170 2090-3189 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Breast Cancer |
| spelling | doaj-art-eb62ad0d9134430f824adde4cda6905a2025-02-03T07:24:58ZengWileyInternational Journal of Breast Cancer2090-31702090-31892015-01-01201510.1155/2015/139349139349Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell LineFaeze Vali0Vahid Changizi1Majid Safa2Technology of Radiology and Radiotherapy Department, Allied Medical Sciences School, Tehran University of Medical Sciences, No. 22, Keshavarz Boulevard, Tehran 14177-44361, IranTechnology of Radiology and Radiotherapy Department, Allied Medical Sciences School, Tehran University of Medical Sciences, No. 22, Keshavarz Boulevard, Tehran 14177-44361, IranAllied Medical Sciences School, Iran University of Medical Sciences, Tehran, IranBackground. Chemotherapy, radiotherapy, and surgery are routine treatments of breast cancer. However, these methods could only improve the living survival. Nowadays the combined therapy including herbals such as crocin is to study for improving breast cancer treatment. The purpose of this study was to evaluate the effects of crocin, paclitaxel, and radiation on MCF-7 cell. Methods. To evaluate the effect of crocin, paclitaxel, and radiation on survival rate of MCF-7 cells MTT assay was done. To investigate the apoptotic effect of experimental groups PI-flow cytometry was used and expression of apoptotic proteins (caspase-7, caspase-9, PARP, and p53) was studied by western blot. Results. This study revealed that the combined therapy of 0.01µmol/mL paclitaxel and 2.5 mg/mL crocin after 48 h could cause IC50 for MCF-7 cell line. This study showed that the combined therapy of 2 Gy gamma radiation with crocin could rise apoptosis in MCF-7 cell line from 21% (related to using 2 Gy gamma radiation alone) to 46.6%. Conclusion. Crocin and paclitaxel and crocin and gamma radiation had synergistic effect on MCF-7 cell line to get more significant apoptosis.http://dx.doi.org/10.1155/2015/139349 |
| spellingShingle | Faeze Vali Vahid Changizi Majid Safa Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell Line International Journal of Breast Cancer |
| title | Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell Line |
| title_full | Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell Line |
| title_fullStr | Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell Line |
| title_full_unstemmed | Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell Line |
| title_short | Synergistic Apoptotic Effect of Crocin and Paclitaxel or Crocin and Radiation on MCF-7 Cells, a Type of Breast Cancer Cell Line |
| title_sort | synergistic apoptotic effect of crocin and paclitaxel or crocin and radiation on mcf 7 cells a type of breast cancer cell line |
| url | http://dx.doi.org/10.1155/2015/139349 |
| work_keys_str_mv | AT faezevali synergisticapoptoticeffectofcrocinandpaclitaxelorcrocinandradiationonmcf7cellsatypeofbreastcancercellline AT vahidchangizi synergisticapoptoticeffectofcrocinandpaclitaxelorcrocinandradiationonmcf7cellsatypeofbreastcancercellline AT majidsafa synergisticapoptoticeffectofcrocinandpaclitaxelorcrocinandradiationonmcf7cellsatypeofbreastcancercellline |