Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In Vitro
A series of in vitro studies were conducted to explore the anti-methanogenic potential of five seaweeds collected from the Indian sea and to optimize the level(s) of incorporation of the most promising seaweed(s) into a straw and concentrate diet to achieve a significant reduction in methane (CH<...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/123 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587891111362560 |
|---|---|
| author | Pradeep Kumar Malik Atul Purshottam Kolte Shraddha Trivedi Govindan Tamilmani Archit Mohapatra Shalini Vaswani Johnson Belevendran Artabandhu Sahoo Achamveetil Gopalakrishnan Raghavendra Bhatta |
| author_facet | Pradeep Kumar Malik Atul Purshottam Kolte Shraddha Trivedi Govindan Tamilmani Archit Mohapatra Shalini Vaswani Johnson Belevendran Artabandhu Sahoo Achamveetil Gopalakrishnan Raghavendra Bhatta |
| author_sort | Pradeep Kumar Malik |
| collection | DOAJ |
| description | A series of in vitro studies were conducted to explore the anti-methanogenic potential of five seaweeds collected from the Indian sea and to optimize the level(s) of incorporation of the most promising seaweed(s) into a straw and concentrate diet to achieve a significant reduction in methane (CH<sub>4</sub>) production without disturbing rumen fermentation characteristics. A chemical composition analysis revealed a notable ash content varying between 55 and 70% in seaweeds. The crude protein content was highly variable and ranged between 3.25 and 15.3% of dry matter. Seaweeds contained appreciable concentrations of tannins and saponins. Among the seaweeds, <i>Spyridia filamentosa</i> exhibited significantly higher CH<sub>4</sub> production, whereas the percentage of CH<sub>4</sub> in total gas was significantly lower in the cases of <i>Kappaphycus alvarezii</i> and <i>Sargassum wightii</i>. The ranking of seaweeds in terms of CH<sub>4</sub> production (mL/g OM) is as follows: <i>Sargassum wightii</i> < <i>Kappaphycus alvarezii</i> < <i>Acanthophora specifera</i> < <i>Padina gymnospora</i> < <i>Spyridia filamentosa</i>. A remarkable decrease of 31–42% in CH<sub>4</sub> production was recorded with the incremental inclusion of <i>Kappaphycus alvarezii</i> at levels of 3–5% of the dry matter in the diet. The addition of <i>Sargassum wightii</i> led to a significant decrease of 36–48% in CH<sub>4</sub> emissions when incorporated at levels of 4–5% of dry matter, respectively. The findings of this study revealed a significant decrease in the numbers of total protozoa and <i>Entodinomorphs</i>, coupled with increasing abundances of sulfate-reducing microbes and minor methanogens. Metagenome data revealed that irrespective of the seaweed and treatment, the predominant microbial phyla included Bacteroidota, Bacillota, Pseudomonadota, Actinomycetota, Fibrobacterota, and Euryarchaeota. The prevalence of <i>Methanobrevibacter</i> was similar across treatments, constituting the majority (~79%) of the archaeal community. The results also demonstrated that the supplementation of <i>Kappaphycus alvarezii</i> and <i>Sargassum wightii</i> did not alter the feed fermentation pattern, and therefore, the reduction in CH<sub>4</sub> production in the present study could not be attributed to it. Animal studies are warranted to validate the extent of reduction in CH<sub>4</sub> production and the key processes involved by supplementation with <i>Kappaphycus alvarezii</i> and <i>Sargassum wightii</i> at the recommended levels. |
| format | Article |
| id | doaj-art-eb451f60862d403d8775706ae068f252 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-eb451f60862d403d8775706ae068f2522025-01-24T13:42:44ZengMDPI AGMicroorganisms2076-26072025-01-0113112310.3390/microorganisms13010123Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In VitroPradeep Kumar Malik0Atul Purshottam Kolte1Shraddha Trivedi2Govindan Tamilmani3Archit Mohapatra4Shalini Vaswani5Johnson Belevendran6Artabandhu Sahoo7Achamveetil Gopalakrishnan8Raghavendra Bhatta9ICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, IndiaICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, IndiaICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, IndiaICAR-Central Marine Fisheries Research Institute, Mandapam Regional Station, Mandapam 623518, IndiaICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, IndiaCollege of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura 281001, IndiaICAR-Central Marine Fisheries Research Institute, Mandapam Regional Station, Mandapam 623518, IndiaICAR-National Institute of Animal Nutrition and Physiology, Bangalore 560030, IndiaICAR-Central Marine Fisheries Research Institute, Kochi 682018, IndiaIndian Council of Agricultural Research, New Delhi 110001, IndiaA series of in vitro studies were conducted to explore the anti-methanogenic potential of five seaweeds collected from the Indian sea and to optimize the level(s) of incorporation of the most promising seaweed(s) into a straw and concentrate diet to achieve a significant reduction in methane (CH<sub>4</sub>) production without disturbing rumen fermentation characteristics. A chemical composition analysis revealed a notable ash content varying between 55 and 70% in seaweeds. The crude protein content was highly variable and ranged between 3.25 and 15.3% of dry matter. Seaweeds contained appreciable concentrations of tannins and saponins. Among the seaweeds, <i>Spyridia filamentosa</i> exhibited significantly higher CH<sub>4</sub> production, whereas the percentage of CH<sub>4</sub> in total gas was significantly lower in the cases of <i>Kappaphycus alvarezii</i> and <i>Sargassum wightii</i>. The ranking of seaweeds in terms of CH<sub>4</sub> production (mL/g OM) is as follows: <i>Sargassum wightii</i> < <i>Kappaphycus alvarezii</i> < <i>Acanthophora specifera</i> < <i>Padina gymnospora</i> < <i>Spyridia filamentosa</i>. A remarkable decrease of 31–42% in CH<sub>4</sub> production was recorded with the incremental inclusion of <i>Kappaphycus alvarezii</i> at levels of 3–5% of the dry matter in the diet. The addition of <i>Sargassum wightii</i> led to a significant decrease of 36–48% in CH<sub>4</sub> emissions when incorporated at levels of 4–5% of dry matter, respectively. The findings of this study revealed a significant decrease in the numbers of total protozoa and <i>Entodinomorphs</i>, coupled with increasing abundances of sulfate-reducing microbes and minor methanogens. Metagenome data revealed that irrespective of the seaweed and treatment, the predominant microbial phyla included Bacteroidota, Bacillota, Pseudomonadota, Actinomycetota, Fibrobacterota, and Euryarchaeota. The prevalence of <i>Methanobrevibacter</i> was similar across treatments, constituting the majority (~79%) of the archaeal community. The results also demonstrated that the supplementation of <i>Kappaphycus alvarezii</i> and <i>Sargassum wightii</i> did not alter the feed fermentation pattern, and therefore, the reduction in CH<sub>4</sub> production in the present study could not be attributed to it. Animal studies are warranted to validate the extent of reduction in CH<sub>4</sub> production and the key processes involved by supplementation with <i>Kappaphycus alvarezii</i> and <i>Sargassum wightii</i> at the recommended levels.https://www.mdpi.com/2076-2607/13/1/123<i>Kappaphycus alvarezii</i>methanemitigationseaweeds<i>Sargassum wightii</i> |
| spellingShingle | Pradeep Kumar Malik Atul Purshottam Kolte Shraddha Trivedi Govindan Tamilmani Archit Mohapatra Shalini Vaswani Johnson Belevendran Artabandhu Sahoo Achamveetil Gopalakrishnan Raghavendra Bhatta Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In Vitro Microorganisms <i>Kappaphycus alvarezii</i> methane mitigation seaweeds <i>Sargassum wightii</i> |
| title | Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In Vitro |
| title_full | Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In Vitro |
| title_fullStr | Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In Vitro |
| title_full_unstemmed | Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In Vitro |
| title_short | Anti-Methanogenic Potential of Seaweeds and Impact on Feed Fermentation and Rumen Microbiome In Vitro |
| title_sort | anti methanogenic potential of seaweeds and impact on feed fermentation and rumen microbiome in vitro |
| topic | <i>Kappaphycus alvarezii</i> methane mitigation seaweeds <i>Sargassum wightii</i> |
| url | https://www.mdpi.com/2076-2607/13/1/123 |
| work_keys_str_mv | AT pradeepkumarmalik antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT atulpurshottamkolte antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT shraddhatrivedi antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT govindantamilmani antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT architmohapatra antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT shalinivaswani antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT johnsonbelevendran antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT artabandhusahoo antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT achamveetilgopalakrishnan antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro AT raghavendrabhatta antimethanogenicpotentialofseaweedsandimpactonfeedfermentationandrumenmicrobiomeinvitro |