Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model
Purpose. Pancreatic cancer is a lethal disease and usually is diagnosed at advanced stages of disease. This study assessed the effects of intratumoral ethanol injection using an endoscopic ultrasound (EUS) probe on the control of pancreatic cancer in a mouse orthotopic xenograft model. Materials and...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2018/7149565 |
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| author | Wen-Ying Zhang Zhen-Dong Jin Feng Liu Hai-Hua Yuan Bin Jiang |
| author_facet | Wen-Ying Zhang Zhen-Dong Jin Feng Liu Hai-Hua Yuan Bin Jiang |
| author_sort | Wen-Ying Zhang |
| collection | DOAJ |
| description | Purpose. Pancreatic cancer is a lethal disease and usually is diagnosed at advanced stages of disease. This study assessed the effects of intratumoral ethanol injection using an endoscopic ultrasound (EUS) probe on the control of pancreatic cancer in a mouse orthotopic xenograft model. Materials and Methods. The subcutaneous and orthotopic human pancreatic cancer cell mouse xenograft models were established. Different concentrations of ethanol (0–95%) were injected into subcutaneous xenograft tumors. In the orthotopic tumor model, ethanol was injected into the tumor lesions under the guidance of a high-frequency EUS probe. Tumor volume, relative tumor volume (RTV), and histopathology were evaluated. The serum amylase level was analyzed at baseline and 24 h after treatment in the orthotopic tumor model. Results. Injection of 40–95% ethanol induced tumor necrosis in the subcutaneous tumor model, while there was no statistical difference between the RTVs of the two groups (P=0.81). In the orthotopic tumor model, the RTV of the 80% ethanol treatment group was less than that of the saline injection group (P<0.01); and histologically, there was a large area of necrosis observed in the 80% ethanol group. The serum amylase level was slightly elevated at 24 h after injection and returned to the baseline level at 7 days. Conclusion. Injection of 80% ethanol into xenograft tumor lesions of orthotopic pancreatic cancer resulted in tumor necrosis, and the procedure was safe and effective. Future studies will further confirm its antitumor activity as well as assess its safety and feasibility. |
| format | Article |
| id | doaj-art-eb406b4632f448b1bd70cb5147d9bcfb |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-eb406b4632f448b1bd70cb5147d9bcfb2025-02-03T07:25:10ZengWileyGastroenterology Research and Practice1687-61211687-630X2018-01-01201810.1155/2018/71495657149565Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft ModelWen-Ying Zhang0Zhen-Dong Jin1Feng Liu2Hai-Hua Yuan3Bin Jiang4Department of Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai, ChinaDepartment of Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, ChinaPurpose. Pancreatic cancer is a lethal disease and usually is diagnosed at advanced stages of disease. This study assessed the effects of intratumoral ethanol injection using an endoscopic ultrasound (EUS) probe on the control of pancreatic cancer in a mouse orthotopic xenograft model. Materials and Methods. The subcutaneous and orthotopic human pancreatic cancer cell mouse xenograft models were established. Different concentrations of ethanol (0–95%) were injected into subcutaneous xenograft tumors. In the orthotopic tumor model, ethanol was injected into the tumor lesions under the guidance of a high-frequency EUS probe. Tumor volume, relative tumor volume (RTV), and histopathology were evaluated. The serum amylase level was analyzed at baseline and 24 h after treatment in the orthotopic tumor model. Results. Injection of 40–95% ethanol induced tumor necrosis in the subcutaneous tumor model, while there was no statistical difference between the RTVs of the two groups (P=0.81). In the orthotopic tumor model, the RTV of the 80% ethanol treatment group was less than that of the saline injection group (P<0.01); and histologically, there was a large area of necrosis observed in the 80% ethanol group. The serum amylase level was slightly elevated at 24 h after injection and returned to the baseline level at 7 days. Conclusion. Injection of 80% ethanol into xenograft tumor lesions of orthotopic pancreatic cancer resulted in tumor necrosis, and the procedure was safe and effective. Future studies will further confirm its antitumor activity as well as assess its safety and feasibility.http://dx.doi.org/10.1155/2018/7149565 |
| spellingShingle | Wen-Ying Zhang Zhen-Dong Jin Feng Liu Hai-Hua Yuan Bin Jiang Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model Gastroenterology Research and Practice |
| title | Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model |
| title_full | Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model |
| title_fullStr | Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model |
| title_full_unstemmed | Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model |
| title_short | Antitumor Activity of Intratumoral Ethanol Injection in an Orthotopic Pancreatic Cancer Cell Mouse Xenograft Model |
| title_sort | antitumor activity of intratumoral ethanol injection in an orthotopic pancreatic cancer cell mouse xenograft model |
| url | http://dx.doi.org/10.1155/2018/7149565 |
| work_keys_str_mv | AT wenyingzhang antitumoractivityofintratumoralethanolinjectioninanorthotopicpancreaticcancercellmousexenograftmodel AT zhendongjin antitumoractivityofintratumoralethanolinjectioninanorthotopicpancreaticcancercellmousexenograftmodel AT fengliu antitumoractivityofintratumoralethanolinjectioninanorthotopicpancreaticcancercellmousexenograftmodel AT haihuayuan antitumoractivityofintratumoralethanolinjectioninanorthotopicpancreaticcancercellmousexenograftmodel AT binjiang antitumoractivityofintratumoralethanolinjectioninanorthotopicpancreaticcancercellmousexenograftmodel |