Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles
Xiaomin Lan,1,* Yi Wang,2,* Ming Yin3,4 1Department of Stomatology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, China; 2Department of Stomatology, The Second People’s Hospital of Taiyuan, Taiyuan, 030002, China; 3Department of Stomatology, Shanxi Bethune...
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Dove Medical Press
2025-01-01
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| author | Lan X Wang Y Yin M |
| author_facet | Lan X Wang Y Yin M |
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| description | Xiaomin Lan,1,* Yi Wang,2,* Ming Yin3,4 1Department of Stomatology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, China; 2Department of Stomatology, The Second People’s Hospital of Taiyuan, Taiyuan, 030002, China; 3Department of Stomatology, Shanxi Bethune Hospital, Shanxi Academy of Medical Science, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, 030032, China; 4Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China*These authors contributed equally to this workCorrespondence: Ming Yin, Email yinm0616@163.comBackground: Regenerating periodontal ligament (PDL) tissue is a vital challenge in dentistry that aims to restore periodontal function and aesthetics. This study explores a tissue engineering strategy that combines polycaprolactone (PCL)/collagen/cellulose acetate electrospun scaffolds with collagen hydrogels to deliver curcumin-loaded ZIF-8 nanoparticles fand periodontal ligament stem cells (PDLSCs).Methods: Scaffolds were fabricated via electrospinningand collagen hydrogels incorporated PDLSCs and curcumin-loaded ZIF-8 nanoparticles (CURZIF-8) were developed using cross-linking. In vitro assays evaluated biocompatibility, anti-inflammatory, and antioxidative properties. In vivo efficacy was assessed in a rat PDL injury model using histological and ELISA analyses examining tissue regeneration and inflammatory cytokine modulation.Results: In vitro studies demonstrated that the scaffolds effectively supported PDLSC viability and migration. CURZIF-8 hydrogels enhanced anti-inflammatory and antioxidative activities. In vivo study showed that the combined scaffold-hydrogel system significantly promoted PDL regeneration. Tissue levels of bFGF, HGF, and TGF-β that are crucial for tissue repair, angiogenesis, and cell proliferation were evaluated. Whereas, pro-inflammatory cytokines TNF-α and IL-6=were downregulated. Histological analysis confirmed the formation of organized PDL structures and improved bone-cementum integration that arekey indicators of successful periodontal regeneration.Conclusion: The developed scaffold-hydrogel system facilitates PDL regeneration by modulating inflammation and promoting pro-healing factor expression. This approach shows promise for advancing periodontal tissue engineering and warrants further investigation in clinical settings. Keywords: periodontal ligament regeneration, periodontal ligament stem cells, electrospun scaffolds, collagen hydrogel, curcumin, ZIF-8 nanoparticles, tissue engineering |
| format | Article |
| id | doaj-art-eaf23df68246470d92c55e27e2a16433 |
| institution | Kabale University |
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| spelling | doaj-art-eaf23df68246470d92c55e27e2a164332025-01-21T16:58:06ZengDove Medical PressInternational Journal of Nanomedicine1178-20132025-01-01Volume 2088790699405Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 NanoparticlesLan XWang YYin MXiaomin Lan,1,* Yi Wang,2,* Ming Yin3,4 1Department of Stomatology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030001, China; 2Department of Stomatology, The Second People’s Hospital of Taiyuan, Taiyuan, 030002, China; 3Department of Stomatology, Shanxi Bethune Hospital, Shanxi Academy of Medical Science, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, 030032, China; 4Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China*These authors contributed equally to this workCorrespondence: Ming Yin, Email yinm0616@163.comBackground: Regenerating periodontal ligament (PDL) tissue is a vital challenge in dentistry that aims to restore periodontal function and aesthetics. This study explores a tissue engineering strategy that combines polycaprolactone (PCL)/collagen/cellulose acetate electrospun scaffolds with collagen hydrogels to deliver curcumin-loaded ZIF-8 nanoparticles fand periodontal ligament stem cells (PDLSCs).Methods: Scaffolds were fabricated via electrospinningand collagen hydrogels incorporated PDLSCs and curcumin-loaded ZIF-8 nanoparticles (CURZIF-8) were developed using cross-linking. In vitro assays evaluated biocompatibility, anti-inflammatory, and antioxidative properties. In vivo efficacy was assessed in a rat PDL injury model using histological and ELISA analyses examining tissue regeneration and inflammatory cytokine modulation.Results: In vitro studies demonstrated that the scaffolds effectively supported PDLSC viability and migration. CURZIF-8 hydrogels enhanced anti-inflammatory and antioxidative activities. In vivo study showed that the combined scaffold-hydrogel system significantly promoted PDL regeneration. Tissue levels of bFGF, HGF, and TGF-β that are crucial for tissue repair, angiogenesis, and cell proliferation were evaluated. Whereas, pro-inflammatory cytokines TNF-α and IL-6=were downregulated. Histological analysis confirmed the formation of organized PDL structures and improved bone-cementum integration that arekey indicators of successful periodontal regeneration.Conclusion: The developed scaffold-hydrogel system facilitates PDL regeneration by modulating inflammation and promoting pro-healing factor expression. This approach shows promise for advancing periodontal tissue engineering and warrants further investigation in clinical settings. Keywords: periodontal ligament regeneration, periodontal ligament stem cells, electrospun scaffolds, collagen hydrogel, curcumin, ZIF-8 nanoparticles, tissue engineeringhttps://www.dovepress.com/enhancing-periodontal-ligament-regeneration-via-pdlsc-delivery-using-e-peer-reviewed-fulltext-article-IJNperiodontal ligament regenerationperiodontal ligament stem cellselectrospun scaffoldscollagen hydrogelcurcuminzif-8 nanoparticlestissue engineering |
| spellingShingle | Lan X Wang Y Yin M Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles International Journal of Nanomedicine periodontal ligament regeneration periodontal ligament stem cells electrospun scaffolds collagen hydrogel curcumin zif-8 nanoparticles tissue engineering |
| title | Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles |
| title_full | Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles |
| title_fullStr | Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles |
| title_full_unstemmed | Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles |
| title_short | Enhancing Periodontal Ligament Regeneration via PDLSC Delivery Using Electrospun PCL/Collagen/Cellulose Acetate Scaffolds and Collagen Hydrogel Incorporated with Curcumin-Loaded ZIF-8 Nanoparticles |
| title_sort | enhancing periodontal ligament regeneration via pdlsc delivery using electrospun pcl collagen cellulose acetate scaffolds and collagen hydrogel incorporated with curcumin loaded zif 8 nanoparticles |
| topic | periodontal ligament regeneration periodontal ligament stem cells electrospun scaffolds collagen hydrogel curcumin zif-8 nanoparticles tissue engineering |
| url | https://www.dovepress.com/enhancing-periodontal-ligament-regeneration-via-pdlsc-delivery-using-e-peer-reviewed-fulltext-article-IJN |
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