Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitors

A library of substituted indolo[2,3-c]quinolone-6-ones was developed as simplified Lamellarin isosters. Synthesis was achieved from indole after a four-step pathway sequence involving iodination, a Suzuki-Miyaura cross-coupling reaction, and a reduction/lactamization sequence. The inhibitory activit...

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Main Authors: Sreenivas Avula, Xudan Peng, Xingfen Lang, Micky Tortorella, Béatrice Josselin, Stéphane Bach, Stephane Bourg, Pascal Bonnet, Frédéric Buron, Sandrine Ruchaud, Sylvain Routier, Cleopatra Neagoie
Format: Article
Language:English
Published: Taylor & Francis Group 2022-12-01
Series:Journal of Enzyme Inhibition and Medicinal Chemistry
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Online Access:https://www.tandfonline.com/doi/10.1080/14756366.2022.2082419
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author Sreenivas Avula
Xudan Peng
Xingfen Lang
Micky Tortorella
Béatrice Josselin
Stéphane Bach
Stephane Bourg
Pascal Bonnet
Frédéric Buron
Sandrine Ruchaud
Sylvain Routier
Cleopatra Neagoie
author_facet Sreenivas Avula
Xudan Peng
Xingfen Lang
Micky Tortorella
Béatrice Josselin
Stéphane Bach
Stephane Bourg
Pascal Bonnet
Frédéric Buron
Sandrine Ruchaud
Sylvain Routier
Cleopatra Neagoie
author_sort Sreenivas Avula
collection DOAJ
description A library of substituted indolo[2,3-c]quinolone-6-ones was developed as simplified Lamellarin isosters. Synthesis was achieved from indole after a four-step pathway sequence involving iodination, a Suzuki-Miyaura cross-coupling reaction, and a reduction/lactamization sequence. The inhibitory activity of the 22 novel derivatives was assessed on Haspin kinase. Two of them possessed an IC50 of 1 and 2 nM with selectivity towards a panel of 10 other kinases including the parent kinases DYRK1A and CLK1. The most selective compound exerted additionally a very interesting cell effect on the osteosarcoma U-2 OS cell line.
format Article
id doaj-art-eae579ca70af4ec7902f09b889e08fbc
institution OA Journals
issn 1475-6366
1475-6374
language English
publishDate 2022-12-01
publisher Taylor & Francis Group
record_format Article
series Journal of Enzyme Inhibition and Medicinal Chemistry
spelling doaj-art-eae579ca70af4ec7902f09b889e08fbc2025-08-20T02:36:41ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742022-12-013711632165010.1080/14756366.2022.2082419Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitorsSreenivas Avula0Xudan Peng1Xingfen Lang2Micky Tortorella3Béatrice Josselin4Stéphane Bach5Stephane Bourg6Pascal Bonnet7Frédéric Buron8Sandrine Ruchaud9Sylvain Routier10Cleopatra Neagoie11Guangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, ChinaGuangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, ChinaGuangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, ChinaGuangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, ChinaSorbonne Université/CNRS UMR8227, Roscoff cedex, FranceSorbonne Université/CNRS UMR8227, Roscoff cedex, FranceInstitut de Chimie Organique et Analytique, Université d’Orléans, UMR CNRS 7311, Orleans, FranceInstitut de Chimie Organique et Analytique, Université d’Orléans, UMR CNRS 7311, Orleans, FranceInstitut de Chimie Organique et Analytique, Université d’Orléans, UMR CNRS 7311, Orleans, FranceSorbonne Université/CNRS UMR8227, Roscoff cedex, FranceInstitut de Chimie Organique et Analytique, Université d’Orléans, UMR CNRS 7311, Orleans, FranceGuangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, ChinaA library of substituted indolo[2,3-c]quinolone-6-ones was developed as simplified Lamellarin isosters. Synthesis was achieved from indole after a four-step pathway sequence involving iodination, a Suzuki-Miyaura cross-coupling reaction, and a reduction/lactamization sequence. The inhibitory activity of the 22 novel derivatives was assessed on Haspin kinase. Two of them possessed an IC50 of 1 and 2 nM with selectivity towards a panel of 10 other kinases including the parent kinases DYRK1A and CLK1. The most selective compound exerted additionally a very interesting cell effect on the osteosarcoma U-2 OS cell line.https://www.tandfonline.com/doi/10.1080/14756366.2022.2082419IndoloquinolineHaspin kinasedockingcell viability
spellingShingle Sreenivas Avula
Xudan Peng
Xingfen Lang
Micky Tortorella
Béatrice Josselin
Stéphane Bach
Stephane Bourg
Pascal Bonnet
Frédéric Buron
Sandrine Ruchaud
Sylvain Routier
Cleopatra Neagoie
Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitors
Journal of Enzyme Inhibition and Medicinal Chemistry
Indoloquinoline
Haspin kinase
docking
cell viability
title Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitors
title_full Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitors
title_fullStr Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitors
title_full_unstemmed Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitors
title_short Design and biological evaluation of substituted 5,7-dihydro-6H-indolo[2,3-c]quinolin-6-one as novel selective Haspin inhibitors
title_sort design and biological evaluation of substituted 5 7 dihydro 6h indolo 2 3 c quinolin 6 one as novel selective haspin inhibitors
topic Indoloquinoline
Haspin kinase
docking
cell viability
url https://www.tandfonline.com/doi/10.1080/14756366.2022.2082419
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