Immunohistochemistry for (Pro)renin Receptor in Humans
The (pro)renin receptor is a multifunctional protein with roles in angiotensin-II-dependent and -independent intracellular cell signaling and roles as an intracellular accessory protein for the vacuolar H+-ATPase, including hormone secretion. While (pro)renin receptor mRNA is widely expressed in var...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2021/8828610 |
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| author | Satoshi Morimoto Noriko Morishima Daisuke Watanabe Yoichiro Kato Noriyuki Shibata Atsuhiro Ichihara |
| author_facet | Satoshi Morimoto Noriko Morishima Daisuke Watanabe Yoichiro Kato Noriyuki Shibata Atsuhiro Ichihara |
| author_sort | Satoshi Morimoto |
| collection | DOAJ |
| description | The (pro)renin receptor is a multifunctional protein with roles in angiotensin-II-dependent and -independent intracellular cell signaling and roles as an intracellular accessory protein for the vacuolar H+-ATPase, including hormone secretion. While (pro)renin receptor mRNA is widely expressed in various human tissues, localization of (pro)renin receptor protein expression has not yet been systemically determined. Therefore, this study localized (pro)renin receptor protein expression in human organs. Systemic immunohistochemical examination of (pro)renin receptor expression was performed in whole body organs of autopsy cases. (Pro)renin receptor immunostaining was observed in the cytoplasm of cells in almost all human organs. It was observed in thyroid follicular epithelial cells, hepatic cells, pancreatic duct epithelial cells, zona glomerulosa and zona reticularis of the cortex and medulla of the adrenal gland, proximal and distal tubules and collecting ducts of the kidney, cardiomyocytes, and skeletal muscle cells. In the brain, (pro)renin receptor staining was detected in neurons throughout all areas, especially in the medulla oblongata, paraventricular nucleus and supraoptic nucleus of the hypothalamus, cerebrum, granular layer of the hippocampus, Purkinje cell layer of the cerebellum, and the pituitary anterior and posterior lobes. In the anterior lobe of the pituitary gland, all types of anterior pituitary hormone-positive cells showed double staining with (pro)renin receptor. These data showed that (pro)renin receptor protein was expressed in almost all organs of the human body. Its expression pattern was not uniform, and cell-specific expression pattern was observed, supporting the notion that (pro)renin receptor plays numerous physiological roles in each human organ. |
| format | Article |
| id | doaj-art-eae455b894b44cd5b1e0e8b1e0866ea0 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-eae455b894b44cd5b1e0e8b1e0866ea02025-02-03T05:48:11ZengWileyInternational Journal of Endocrinology1687-83371687-83452021-01-01202110.1155/2021/88286108828610Immunohistochemistry for (Pro)renin Receptor in HumansSatoshi Morimoto0Noriko Morishima1Daisuke Watanabe2Yoichiro Kato3Noriyuki Shibata4Atsuhiro Ichihara5Department of Endocrinology and Hypertension, Tokyo Women’s Medical University, Tokyo, JapanDepartment of Endocrinology and Hypertension, Tokyo Women’s Medical University, Tokyo, JapanDepartment of Endocrinology and Hypertension, Tokyo Women’s Medical University, Tokyo, JapanDepartment of Pathology, Tokyo Women’s Medical University, Tokyo, JapanDepartment of Pathology, Tokyo Women’s Medical University, Tokyo, JapanDepartment of Endocrinology and Hypertension, Tokyo Women’s Medical University, Tokyo, JapanThe (pro)renin receptor is a multifunctional protein with roles in angiotensin-II-dependent and -independent intracellular cell signaling and roles as an intracellular accessory protein for the vacuolar H+-ATPase, including hormone secretion. While (pro)renin receptor mRNA is widely expressed in various human tissues, localization of (pro)renin receptor protein expression has not yet been systemically determined. Therefore, this study localized (pro)renin receptor protein expression in human organs. Systemic immunohistochemical examination of (pro)renin receptor expression was performed in whole body organs of autopsy cases. (Pro)renin receptor immunostaining was observed in the cytoplasm of cells in almost all human organs. It was observed in thyroid follicular epithelial cells, hepatic cells, pancreatic duct epithelial cells, zona glomerulosa and zona reticularis of the cortex and medulla of the adrenal gland, proximal and distal tubules and collecting ducts of the kidney, cardiomyocytes, and skeletal muscle cells. In the brain, (pro)renin receptor staining was detected in neurons throughout all areas, especially in the medulla oblongata, paraventricular nucleus and supraoptic nucleus of the hypothalamus, cerebrum, granular layer of the hippocampus, Purkinje cell layer of the cerebellum, and the pituitary anterior and posterior lobes. In the anterior lobe of the pituitary gland, all types of anterior pituitary hormone-positive cells showed double staining with (pro)renin receptor. These data showed that (pro)renin receptor protein was expressed in almost all organs of the human body. Its expression pattern was not uniform, and cell-specific expression pattern was observed, supporting the notion that (pro)renin receptor plays numerous physiological roles in each human organ.http://dx.doi.org/10.1155/2021/8828610 |
| spellingShingle | Satoshi Morimoto Noriko Morishima Daisuke Watanabe Yoichiro Kato Noriyuki Shibata Atsuhiro Ichihara Immunohistochemistry for (Pro)renin Receptor in Humans International Journal of Endocrinology |
| title | Immunohistochemistry for (Pro)renin Receptor in Humans |
| title_full | Immunohistochemistry for (Pro)renin Receptor in Humans |
| title_fullStr | Immunohistochemistry for (Pro)renin Receptor in Humans |
| title_full_unstemmed | Immunohistochemistry for (Pro)renin Receptor in Humans |
| title_short | Immunohistochemistry for (Pro)renin Receptor in Humans |
| title_sort | immunohistochemistry for pro renin receptor in humans |
| url | http://dx.doi.org/10.1155/2021/8828610 |
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