Oxidative, Inflammatory, and Constipation Stress Modulation by a Heteropolysaccharide from <i>Lacticaseibacillus rhamnosus</i> CRL75
<i>Lacticaseibacillus</i> (<i>L</i>.) <i>rhamnosus</i> CRL75 is a lactic acid bacterium (LAB) isolated from local dairy products, demonstrating significant adaptation in skimmed milk (FM75). In this context, CRL75 exhibited high microbial growth (3.63 ± 0.18 log C...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/4/224 |
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| Summary: | <i>Lacticaseibacillus</i> (<i>L</i>.) <i>rhamnosus</i> CRL75 is a lactic acid bacterium (LAB) isolated from local dairy products, demonstrating significant adaptation in skimmed milk (FM75). In this context, CRL75 exhibited high microbial growth (3.63 ± 0.18 log CFU·mL<sup>−1</sup>), strong acidification (9.20 ± 0.10 g·L<sup>−1</sup> lactic acid, and 2.40 ± 0.10 pH units), and increased viscosity in FM75 after 16 h of fermentation. Additionally, this LAB strain produces both capsular polysaccharides (CPS+) and extracellular polysaccharides (EPS75), contributing to a ropy phenotype (>10 cm). The purified EPS75 (70.70 ± 3.25 mg·L<sup>−1</sup>) displayed low molecular weight (12.7 kDa), with galactose and glucose as its primary monomers in a 4:1 ratio. In aqueous environments, EPS75 exhibited an extended size (147 nm), a random coil structure, and macromolecular aggregation. Furthermore, vibrational spectroscopy confirmed the presence of a neutral EPS with high thermal stability. Additionally, EPS75 exhibited dose-dependent antioxidant activity, effectively reducing metal ions (Fe<sup>3+</sup>, Mo<sup>6+</sup>, and Mn<sup>7+</sup>) and stabilizing radicals (ABTS<sup>•+</sup>, HO<sup>•</sup>, O<sub>2</sub><sup>•−</sup>, and HOO<sup>•</sup>). The biopolymer also demonstrated immunostimulatory and anti-inflammatory effects in RAW 264.7 cells. In vivo assays using Balb/c mice indicated that both EPS75 and FM75 prevented constipation, suggesting their potential as natural and safe agents for constipation-related disorders. Due to its viscosifying and health-promoting attributes, CRL75 offers promising applications for functional dairy products. |
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| ISSN: | 2311-5637 |