High-accuracy crRNA array assembly strategy for multiplex CRISPR

High-accuracy crRNA array assembly strategy for multiplex CRISPR

Simultaneous targeting of multiple loci with the CRISPR system, a tool known as multiplex CRISPR, offers greater feasibility for manipulating and elucidating the intricate and redundant endogenous networks underlying complex cellular functions. Owing to the versatility of continuously emerging Cas n...

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Bibliographic Details
Main Authors: Xiangtong Zhao, Lixian Yang, Peng Li, Zijing Cheng, Yongshi Jia, Limin Luo, Aihong Bi, Hanchu Xiong, Haibo Zhang, Hongen Xu, Jinrui Zhang, Yaodong Zhang
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA Editing
multiplex CRISPR
crRNA
CRISPR array
Golden Gate Assembly
AsCas12a
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253124003159
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