Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells
The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. Rutin...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/382350 |
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| author | Xiaofang Zhang Jinhui Song Xiaopeng Shi Shan Miao Yan Li Aidong Wen |
| author_facet | Xiaofang Zhang Jinhui Song Xiaopeng Shi Shan Miao Yan Li Aidong Wen |
| author_sort | Xiaofang Zhang |
| collection | DOAJ |
| description | The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. Rutin and glucuronidated rutin were absorbed differently by the basolateral and apical membranes, and rutin showed differential permeability through the apical and basolateral sides. Approximately 33% of the rutin was metabolized to glucuronidated rutin, and the intracellular concentration of glucuronidated rutin was much lower than that of parent rutin. P-glycoprotein and multidrug-resistant proteins 2 and 3 were involved in the transmembrane transport and intracellular accumulation of rutin by Caco-2 cells. These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and that metabolic enzymes are important for this process. |
| format | Article |
| id | doaj-art-ead2f39662ef40729afafa687fa6c88c |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ead2f39662ef40729afafa687fa6c88c2025-02-03T05:50:41ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/382350382350Absorption and Metabolism Characteristics of Rutin in Caco-2 CellsXiaofang Zhang0Jinhui Song1Xiaopeng Shi2Shan Miao3Yan Li4Aidong Wen5Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaan’xi 710032, ChinaDepartment of Otolaryngology, Shenyang 463 Hospital, Xiao He Yan Road 46, Shenyang, Liaoning 110042, ChinaDepartment of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaan’xi 710032, ChinaSchool of Pharmacy, Institute of Materia Medica, Fourth Military Medical University, Xi’an, Shaan’xi 710032, ChinaDepartment of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaan’xi 710032, ChinaDepartment of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaan’xi 710032, ChinaThe intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. Rutin and glucuronidated rutin were absorbed differently by the basolateral and apical membranes, and rutin showed differential permeability through the apical and basolateral sides. Approximately 33% of the rutin was metabolized to glucuronidated rutin, and the intracellular concentration of glucuronidated rutin was much lower than that of parent rutin. P-glycoprotein and multidrug-resistant proteins 2 and 3 were involved in the transmembrane transport and intracellular accumulation of rutin by Caco-2 cells. These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and that metabolic enzymes are important for this process.http://dx.doi.org/10.1155/2013/382350 |
| spellingShingle | Xiaofang Zhang Jinhui Song Xiaopeng Shi Shan Miao Yan Li Aidong Wen Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells The Scientific World Journal |
| title | Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells |
| title_full | Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells |
| title_fullStr | Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells |
| title_full_unstemmed | Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells |
| title_short | Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells |
| title_sort | absorption and metabolism characteristics of rutin in caco 2 cells |
| url | http://dx.doi.org/10.1155/2013/382350 |
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