Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension
The functions of genes involved in idiopathic portal hypertension (IPH) remain unidentified. The present study was undertaken to identify the functions of genes expressed in blood samples from patients with IPH through comprehensive analysis of gene expression using DNA microarrays. The data were c...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/349215 |
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| author | Kohei Kotani Joji Kawabe Hiroyasu Morikawa Tomohiko Akahoshi Makoto Hashizume Susumu Shiomi |
| author_facet | Kohei Kotani Joji Kawabe Hiroyasu Morikawa Tomohiko Akahoshi Makoto Hashizume Susumu Shiomi |
| author_sort | Kohei Kotani |
| collection | DOAJ |
| description | The functions of genes involved in idiopathic portal hypertension (IPH) remain unidentified.
The present study was undertaken to identify the functions of genes expressed in blood
samples from patients with IPH through comprehensive analysis of gene expression using
DNA microarrays. The data were compared with data from healthy individuals to explore the
functions of genes showing increased or decreased expression in patients with IPH. In cluster
analysis, no dominant probe group was shown to differ between patients with IPH and healthy
controls. In functional annotation analysis using the Database for Annotation Visualization
and Integrated Discovery tool, clusters showing dysfunction in patients with IPH involved
gene terms related to the immune system. Analysis using network-based pathways revealed
decreased expression of adenosine deaminase, ectonucleoside triphosphate
diphosphohydrolase 4, ATP-binding cassette, subfamily C, member 1, transforming growth
factor-β, and prostaglandin E receptor 2; increased expression of cytochrome P450, family 4,
subfamily F, polypeptide 3, and glutathione peroxidase 3; and abnormalities in the immune
system, nucleic acid metabolism, arachidonic acid/leukotriene pathways, and biological
processes. These results suggested that IPH involved compromised function of
immunocompetent cells and that such dysfunction may be associated with abnormalities in
nucleic acid metabolism and arachidonic acid/leukotriene-related synthesis/metabolism. |
| format | Article |
| id | doaj-art-eac9ec9e2c15454fa230304facd2df35 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-eac9ec9e2c15454fa230304facd2df352025-02-03T01:03:30ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/349215349215Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal HypertensionKohei Kotani0Joji Kawabe1Hiroyasu Morikawa2Tomohiko Akahoshi3Makoto Hashizume4Susumu Shiomi5Department of Nuclear Medicine, Graduate School of Medicine, Osaka City University, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, JapanDepartment of Nuclear Medicine, Graduate School of Medicine, Osaka City University, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, JapanDepartment of Hepatology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, JapanDepartment of Disaster and Emergency Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, JapanDepartment of Disaster and Emergency Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, JapanDepartment of Nuclear Medicine, Graduate School of Medicine, Osaka City University, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, JapanThe functions of genes involved in idiopathic portal hypertension (IPH) remain unidentified. The present study was undertaken to identify the functions of genes expressed in blood samples from patients with IPH through comprehensive analysis of gene expression using DNA microarrays. The data were compared with data from healthy individuals to explore the functions of genes showing increased or decreased expression in patients with IPH. In cluster analysis, no dominant probe group was shown to differ between patients with IPH and healthy controls. In functional annotation analysis using the Database for Annotation Visualization and Integrated Discovery tool, clusters showing dysfunction in patients with IPH involved gene terms related to the immune system. Analysis using network-based pathways revealed decreased expression of adenosine deaminase, ectonucleoside triphosphate diphosphohydrolase 4, ATP-binding cassette, subfamily C, member 1, transforming growth factor-β, and prostaglandin E receptor 2; increased expression of cytochrome P450, family 4, subfamily F, polypeptide 3, and glutathione peroxidase 3; and abnormalities in the immune system, nucleic acid metabolism, arachidonic acid/leukotriene pathways, and biological processes. These results suggested that IPH involved compromised function of immunocompetent cells and that such dysfunction may be associated with abnormalities in nucleic acid metabolism and arachidonic acid/leukotriene-related synthesis/metabolism.http://dx.doi.org/10.1155/2015/349215 |
| spellingShingle | Kohei Kotani Joji Kawabe Hiroyasu Morikawa Tomohiko Akahoshi Makoto Hashizume Susumu Shiomi Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension Mediators of Inflammation |
| title | Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension |
| title_full | Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension |
| title_fullStr | Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension |
| title_full_unstemmed | Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension |
| title_short | Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension |
| title_sort | comprehensive screening of gene function and networks by dna microarray analysis in japanese patients with idiopathic portal hypertension |
| url | http://dx.doi.org/10.1155/2015/349215 |
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