Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing Voltammetry
17α-Ethinyl estradiol (EE2), which is used worldwide in the treatment of some cancers and as a contraceptive, is often found in aquatic systems and is considered a pharmaceutically active compound (PhACs) in the environment. Current methods for the determination of this compound, such as chromatogra...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/3217080 |
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| author | Chalder Nogueira Nunes Lucas Ely Pauluk Maria Lurdes Felsner Vanessa Egéa dos Anjos Sueli Pércio Quináia |
| author_facet | Chalder Nogueira Nunes Lucas Ely Pauluk Maria Lurdes Felsner Vanessa Egéa dos Anjos Sueli Pércio Quináia |
| author_sort | Chalder Nogueira Nunes |
| collection | DOAJ |
| description | 17α-Ethinyl estradiol (EE2), which is used worldwide in the treatment of some cancers and as a contraceptive, is often found in aquatic systems and is considered a pharmaceutically active compound (PhACs) in the environment. Current methods for the determination of this compound, such as chromatography, are expensive and lengthy and require large amounts of toxic organic solvents. In this work, a voltammetric procedure is developed and validated as a screening tool for detecting EE2 in water samples without prior extraction, clean-up, or derivatization steps. Application of the method we elaborate here to EE2 analysis is unprecedented. EE2 detection was carried out using differential pulse adsorptive cathodic stripping voltammetry (DP AdCSV) with a hanging mercury drop electrode (HMDE) in pH 7.0 Britton-Robinson buffer. The electrochemical process of EE2 reduction was investigated by cyclic voltammetry at different scan rates. Electroreduction of the hormone on a mercury electrode exhibited a peak at −1.16±0.02 V versus Ag/AgCl. The experimental parameters were as follows: −0.7 V accumulation potential, 150 s accumulation time, and 60 mV s−1 scan rate. The limit of detection was 0.49 μg L−1 for a preconcentration time of 150 s. Relative standard deviations were less than 13%. The method was applied to the detection of EE2 in water samples with recoveries ranging from 93.7 to 102.5%. |
| format | Article |
| id | doaj-art-eaa25320095f4367a3579f26b217a803 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-eaa25320095f4367a3579f26b217a8032025-02-03T05:59:00ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732016-01-01201610.1155/2016/32170803217080Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing VoltammetryChalder Nogueira Nunes0Lucas Ely Pauluk1Maria Lurdes Felsner2Vanessa Egéa dos Anjos3Sueli Pércio Quináia4Departamento de Química, Universidade Estadual do Centro-Oeste, 85040-080 Guarapuava, PR, BrazilDepartamento de Química, Universidade Estadual do Centro-Oeste, 85040-080 Guarapuava, PR, BrazilDepartamento de Química, Universidade Estadual do Centro-Oeste, 85040-080 Guarapuava, PR, BrazilDepartamento de Química, Universidade Estadual de Ponta Grossa, 84030-900 Ponta Grossa, PR, BrazilDepartamento de Química, Universidade Estadual do Centro-Oeste, 85040-080 Guarapuava, PR, Brazil17α-Ethinyl estradiol (EE2), which is used worldwide in the treatment of some cancers and as a contraceptive, is often found in aquatic systems and is considered a pharmaceutically active compound (PhACs) in the environment. Current methods for the determination of this compound, such as chromatography, are expensive and lengthy and require large amounts of toxic organic solvents. In this work, a voltammetric procedure is developed and validated as a screening tool for detecting EE2 in water samples without prior extraction, clean-up, or derivatization steps. Application of the method we elaborate here to EE2 analysis is unprecedented. EE2 detection was carried out using differential pulse adsorptive cathodic stripping voltammetry (DP AdCSV) with a hanging mercury drop electrode (HMDE) in pH 7.0 Britton-Robinson buffer. The electrochemical process of EE2 reduction was investigated by cyclic voltammetry at different scan rates. Electroreduction of the hormone on a mercury electrode exhibited a peak at −1.16±0.02 V versus Ag/AgCl. The experimental parameters were as follows: −0.7 V accumulation potential, 150 s accumulation time, and 60 mV s−1 scan rate. The limit of detection was 0.49 μg L−1 for a preconcentration time of 150 s. Relative standard deviations were less than 13%. The method was applied to the detection of EE2 in water samples with recoveries ranging from 93.7 to 102.5%.http://dx.doi.org/10.1155/2016/3217080 |
| spellingShingle | Chalder Nogueira Nunes Lucas Ely Pauluk Maria Lurdes Felsner Vanessa Egéa dos Anjos Sueli Pércio Quináia Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing Voltammetry Journal of Analytical Methods in Chemistry |
| title | Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing Voltammetry |
| title_full | Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing Voltammetry |
| title_fullStr | Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing Voltammetry |
| title_full_unstemmed | Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing Voltammetry |
| title_short | Rapid Screening Method for Detecting Ethinyl Estradiol in Natural Water Employing Voltammetry |
| title_sort | rapid screening method for detecting ethinyl estradiol in natural water employing voltammetry |
| url | http://dx.doi.org/10.1155/2016/3217080 |
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