Vascular Guidance: Microstructural Scaffold Patterning for Inductive Neovascularization
Current tissue engineering techniques are limited by inadequate vascularisation and perfusion of cell-scaffold constructs. Microstructural patterning through biomimetic vascular channels within a polymer scaffold might induce neovascularization, allowing fabrication of large engineered constructs. T...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/547247 |
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| author | Daniel Muller Harvey Chim Augustinus Bader Matthew Whiteman Jan-Thorsten Schantz |
| author_facet | Daniel Muller Harvey Chim Augustinus Bader Matthew Whiteman Jan-Thorsten Schantz |
| author_sort | Daniel Muller |
| collection | DOAJ |
| description | Current tissue engineering techniques are limited by inadequate vascularisation and perfusion of cell-scaffold constructs. Microstructural patterning through biomimetic vascular channels within a polymer scaffold might induce neovascularization, allowing fabrication of large engineered constructs.
The network of vascular channels within a frontal-parietal defect in a patient, originating from the anterior branch of the middle meningeal artery, was modeled using computer-aided design (CAD) techniques and subsequently incorporated into polycaprolactone (PCL) scaffolds fabricated using fused deposition modeling (FDM). Bone marrow-derived mesenchymal stem cells (MSCs) were seeded onto the scaffolds and implanted into a rat model, with an arteriovenous bundle inserted at the proximal extent of the vascular network. After 3 weeks, scaffolds were elevated as a prefabricated composite tissue-polymer flap and transferred using microsurgical technique. Histological examination of explanted scaffolds revealed vascular ingrowth along patterned channels, with abundant capillary and connective tissue formation throughout experimental scaffolds, while control scaffolds showed only granulation tissue. All prefabricated constructs transferred as free flaps survived and were viable. We term this concept “vascular guidance,” whereby neovascularization is guided through customized channels in a scaffold. Our technique might potentially allow fabrication of much larger tissue-engineered constructs than current technologies allow, as well as allowing tailored construct fabrication with a patient-specific vessel network based on CT scan data and CAD technology. |
| format | Article |
| id | doaj-art-ea885edfa6da41d1ba7a0b15680c8d21 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ea885edfa6da41d1ba7a0b15680c8d212025-02-03T01:12:06ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/547247547247Vascular Guidance: Microstructural Scaffold Patterning for Inductive NeovascularizationDaniel Muller0Harvey Chim1Augustinus Bader2Matthew Whiteman3Jan-Thorsten Schantz4Department of Plastic, Reconstructive and Handsurgery, Klinikum rechts der Isar, Technische Universität München, 80333 München, GermanyDepartment of Plastic Surgery, Case Western Reserve University, Cleveland, OH 44106, USAZentrum für Stammzellbiologie und Biotechnologie, Universität Leipzig, GermanyPeninsula Medical School, University of Exeter, Exeter EX4 4QJ, UKDepartment of Plastic, Reconstructive and Handsurgery, Klinikum rechts der Isar, Technische Universität München, 80333 München, GermanyCurrent tissue engineering techniques are limited by inadequate vascularisation and perfusion of cell-scaffold constructs. Microstructural patterning through biomimetic vascular channels within a polymer scaffold might induce neovascularization, allowing fabrication of large engineered constructs. The network of vascular channels within a frontal-parietal defect in a patient, originating from the anterior branch of the middle meningeal artery, was modeled using computer-aided design (CAD) techniques and subsequently incorporated into polycaprolactone (PCL) scaffolds fabricated using fused deposition modeling (FDM). Bone marrow-derived mesenchymal stem cells (MSCs) were seeded onto the scaffolds and implanted into a rat model, with an arteriovenous bundle inserted at the proximal extent of the vascular network. After 3 weeks, scaffolds were elevated as a prefabricated composite tissue-polymer flap and transferred using microsurgical technique. Histological examination of explanted scaffolds revealed vascular ingrowth along patterned channels, with abundant capillary and connective tissue formation throughout experimental scaffolds, while control scaffolds showed only granulation tissue. All prefabricated constructs transferred as free flaps survived and were viable. We term this concept “vascular guidance,” whereby neovascularization is guided through customized channels in a scaffold. Our technique might potentially allow fabrication of much larger tissue-engineered constructs than current technologies allow, as well as allowing tailored construct fabrication with a patient-specific vessel network based on CT scan data and CAD technology.http://dx.doi.org/10.4061/2011/547247 |
| spellingShingle | Daniel Muller Harvey Chim Augustinus Bader Matthew Whiteman Jan-Thorsten Schantz Vascular Guidance: Microstructural Scaffold Patterning for Inductive Neovascularization Stem Cells International |
| title | Vascular Guidance: Microstructural Scaffold Patterning for Inductive Neovascularization |
| title_full | Vascular Guidance: Microstructural Scaffold Patterning for Inductive Neovascularization |
| title_fullStr | Vascular Guidance: Microstructural Scaffold Patterning for Inductive Neovascularization |
| title_full_unstemmed | Vascular Guidance: Microstructural Scaffold Patterning for Inductive Neovascularization |
| title_short | Vascular Guidance: Microstructural Scaffold Patterning for Inductive Neovascularization |
| title_sort | vascular guidance microstructural scaffold patterning for inductive neovascularization |
| url | http://dx.doi.org/10.4061/2011/547247 |
| work_keys_str_mv | AT danielmuller vascularguidancemicrostructuralscaffoldpatterningforinductiveneovascularization AT harveychim vascularguidancemicrostructuralscaffoldpatterningforinductiveneovascularization AT augustinusbader vascularguidancemicrostructuralscaffoldpatterningforinductiveneovascularization AT matthewwhiteman vascularguidancemicrostructuralscaffoldpatterningforinductiveneovascularization AT janthorstenschantz vascularguidancemicrostructuralscaffoldpatterningforinductiveneovascularization |