Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation
Electroacupuncture (EA) is reported to effectively relieve the central poststroke pain (CPSP). However, the underlying mechanism remains unclear. The present study investigated the detailed mechanisms of action of EA treatment at different frequencies for CPSP. A CPSP model was established with a si...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/1437148 |
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| author | Gui-Hua Tian Shan-Shan Tao Man-Tang Chen Yu-Sang Li You-Ping Li Hong-Cai Shang Xiao-Yi Tang Jian-Xin Chen He-Bin Tang |
| author_facet | Gui-Hua Tian Shan-Shan Tao Man-Tang Chen Yu-Sang Li You-Ping Li Hong-Cai Shang Xiao-Yi Tang Jian-Xin Chen He-Bin Tang |
| author_sort | Gui-Hua Tian |
| collection | DOAJ |
| description | Electroacupuncture (EA) is reported to effectively relieve the central poststroke pain (CPSP). However, the underlying mechanism remains unclear. The present study investigated the detailed mechanisms of action of EA treatment at different frequencies for CPSP. A CPSP model was established with a single collagenase injection to the left ventral posterolateral nucleus of the thalamus. The EA-treated groups then received EA treatment at frequency of 2, 2/15, or 15 Hz for 30 min daily for five days. The pain-related behavioral responses, neuronal apoptosis, glial activation, and the expression of pain signal transmission-related factors (β-catenin, COX-2, and NK-1R) were assessed using behavioral tests, Nissl staining, TUNEL staining, and immunohistochemical staining, respectively. The low-frequency EA treatment significantly (1) reduced brain tissue damage and hematoma sizes and (2) inhibited neuronal apoptosis, thereby exerting abirritative effects. Meanwhile, the high-frequency EA treatment induced a greater inhibition of the aberrant astrocyte activation, accompanied by the downregulation of the expressions of COX-2, β-catenin, and subsequently NK-1R, thereby alleviating inflammation and producing strong analgesic effects. Together, these findings suggest that CPSP is closely related to pathological changes of the neocortex and hippocampus. EA treatments at different frequencies may exert abirritative effects by inhibiting brain neuronal apoptosis and aberrant astrocyte activation in the brain. |
| format | Article |
| id | doaj-art-ea62f010e40e4f0e9cce10c791da7aad |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-ea62f010e40e4f0e9cce10c791da7aad2025-02-03T06:04:44ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/14371481437148Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte ActivationGui-Hua Tian0Shan-Shan Tao1Man-Tang Chen2Yu-Sang Li3You-Ping Li4Hong-Cai Shang5Xiao-Yi Tang6Jian-Xin Chen7He-Bin Tang8Key Laboratory of Chinese Internal Medicine of MOE, Beijing Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDepartment of Pharmacology, School of Pharmaceutical Sciences, South-Central University for Nationalities, No. 182, Minyuan Road, Wuhan 430074, ChinaDepartment of Pharmacology, School of Pharmaceutical Sciences, South-Central University for Nationalities, No. 182, Minyuan Road, Wuhan 430074, ChinaDepartment of Pharmacology, School of Pharmaceutical Sciences, South-Central University for Nationalities, No. 182, Minyuan Road, Wuhan 430074, ChinaChinese Evidence-Based Medicine Center, West China Hospital, Sichuan University, Sichuan 610041, ChinaKey Laboratory of Chinese Internal Medicine of MOE, Beijing Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of MOE, Beijing Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaKey Laboratory of Chinese Internal Medicine of MOE, Beijing Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDepartment of Pharmacology, School of Pharmaceutical Sciences, South-Central University for Nationalities, No. 182, Minyuan Road, Wuhan 430074, ChinaElectroacupuncture (EA) is reported to effectively relieve the central poststroke pain (CPSP). However, the underlying mechanism remains unclear. The present study investigated the detailed mechanisms of action of EA treatment at different frequencies for CPSP. A CPSP model was established with a single collagenase injection to the left ventral posterolateral nucleus of the thalamus. The EA-treated groups then received EA treatment at frequency of 2, 2/15, or 15 Hz for 30 min daily for five days. The pain-related behavioral responses, neuronal apoptosis, glial activation, and the expression of pain signal transmission-related factors (β-catenin, COX-2, and NK-1R) were assessed using behavioral tests, Nissl staining, TUNEL staining, and immunohistochemical staining, respectively. The low-frequency EA treatment significantly (1) reduced brain tissue damage and hematoma sizes and (2) inhibited neuronal apoptosis, thereby exerting abirritative effects. Meanwhile, the high-frequency EA treatment induced a greater inhibition of the aberrant astrocyte activation, accompanied by the downregulation of the expressions of COX-2, β-catenin, and subsequently NK-1R, thereby alleviating inflammation and producing strong analgesic effects. Together, these findings suggest that CPSP is closely related to pathological changes of the neocortex and hippocampus. EA treatments at different frequencies may exert abirritative effects by inhibiting brain neuronal apoptosis and aberrant astrocyte activation in the brain.http://dx.doi.org/10.1155/2016/1437148 |
| spellingShingle | Gui-Hua Tian Shan-Shan Tao Man-Tang Chen Yu-Sang Li You-Ping Li Hong-Cai Shang Xiao-Yi Tang Jian-Xin Chen He-Bin Tang Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation Neural Plasticity |
| title | Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation |
| title_full | Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation |
| title_fullStr | Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation |
| title_full_unstemmed | Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation |
| title_short | Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation |
| title_sort | electroacupuncture treatment alleviates central poststroke pain by inhibiting brain neuronal apoptosis and aberrant astrocyte activation |
| url | http://dx.doi.org/10.1155/2016/1437148 |
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