Fiber-tip photonic crystal for real-time referenced biosensing in serum
Fiber optic sensors have become increasingly well-established due to the many advantages they provide such as immunity to electromagnetic interference, multiplexing capabilities, and remote sensing. The coupling of light with a transducer at the tip of the optical fiber enables the detection of phys...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Biosensors and Bioelectronics: X |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137024001377 |
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| author | Mathias Dolci Paco Dreverman Mildred S. Cano-Velázquez Arthur L. Hendriks Emiel Veth P.J. van Veldhoven Andrea Fiore Peter Zijlstra |
| author_facet | Mathias Dolci Paco Dreverman Mildred S. Cano-Velázquez Arthur L. Hendriks Emiel Veth P.J. van Veldhoven Andrea Fiore Peter Zijlstra |
| author_sort | Mathias Dolci |
| collection | DOAJ |
| description | Fiber optic sensors have become increasingly well-established due to the many advantages they provide such as immunity to electromagnetic interference, multiplexing capabilities, and remote sensing. The coupling of light with a transducer at the tip of the optical fiber enables the detection of physical and biological parameters. 2D photonic crystals (PhC) can be designed to feature guided-mode resonances (GMR) characterized by a strong electric field at the PhC surface, providing a suitable tool for the detection of local refractive index variations (e.g. biomolecule adsorption). Here, we demonstrate the use of a PhC transferred to the tip of a single-mode fiber for biosensing. The control of surface chemistry provides a sensitive platform for the molecular recognition of antibody biomarkers. By integrating the fiber in a continuous flow platform, the real-time detection of anti-IgG in undiluted serum was achieved, with a limit of detection down to 60 pM. Moreover, the use of a reference channel is demonstrated to correct for signal drifts in real-time due to changes in bulk refractive index. These referenced fiber-tip PhC biosensors may pave the way for fluidic integrated systems in environmental, industrial, and healthcare applications, and open up the possibility of biosensing in the human body by integrating them into catheters. |
| format | Article |
| id | doaj-art-ea5c2627e12d4ae59b136c65186b132b |
| institution | Kabale University |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-ea5c2627e12d4ae59b136c65186b132b2025-01-22T05:43:55ZengElsevierBiosensors and Bioelectronics: X2590-13702025-03-0122100573Fiber-tip photonic crystal for real-time referenced biosensing in serumMathias Dolci0Paco Dreverman1Mildred S. Cano-Velázquez2Arthur L. Hendriks3Emiel Veth4P.J. van Veldhoven5Andrea Fiore6Peter Zijlstra7Department of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the Netherlands; Eindhoven Hendrik Casimir Institute, Eindhoven University of Technology, 5600, MB, Eindhoven, the Netherlands; Corresponding author. Department of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB Eindhoven the NetherlandsDepartment of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the NetherlandsDepartment of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the Netherlands; Eindhoven Hendrik Casimir Institute, Eindhoven University of Technology, 5600, MB, Eindhoven, the NetherlandsDepartment of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the Netherlands; Eindhoven Hendrik Casimir Institute, Eindhoven University of Technology, 5600, MB, Eindhoven, the NetherlandsDepartment of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the NetherlandsDepartment of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the NetherlandsDepartment of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the Netherlands; Eindhoven Hendrik Casimir Institute, Eindhoven University of Technology, 5600, MB, Eindhoven, the NetherlandsDepartment of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB, Eindhoven, the Netherlands; Eindhoven Hendrik Casimir Institute, Eindhoven University of Technology, 5600, MB, Eindhoven, the Netherlands; Corresponding author. Department of Applied Physics and Science Education, Eindhoven University of Technology, 5600, MB Eindhoven the Netherlands.Fiber optic sensors have become increasingly well-established due to the many advantages they provide such as immunity to electromagnetic interference, multiplexing capabilities, and remote sensing. The coupling of light with a transducer at the tip of the optical fiber enables the detection of physical and biological parameters. 2D photonic crystals (PhC) can be designed to feature guided-mode resonances (GMR) characterized by a strong electric field at the PhC surface, providing a suitable tool for the detection of local refractive index variations (e.g. biomolecule adsorption). Here, we demonstrate the use of a PhC transferred to the tip of a single-mode fiber for biosensing. The control of surface chemistry provides a sensitive platform for the molecular recognition of antibody biomarkers. By integrating the fiber in a continuous flow platform, the real-time detection of anti-IgG in undiluted serum was achieved, with a limit of detection down to 60 pM. Moreover, the use of a reference channel is demonstrated to correct for signal drifts in real-time due to changes in bulk refractive index. These referenced fiber-tip PhC biosensors may pave the way for fluidic integrated systems in environmental, industrial, and healthcare applications, and open up the possibility of biosensing in the human body by integrating them into catheters.http://www.sciencedirect.com/science/article/pii/S2590137024001377 |
| spellingShingle | Mathias Dolci Paco Dreverman Mildred S. Cano-Velázquez Arthur L. Hendriks Emiel Veth P.J. van Veldhoven Andrea Fiore Peter Zijlstra Fiber-tip photonic crystal for real-time referenced biosensing in serum Biosensors and Bioelectronics: X |
| title | Fiber-tip photonic crystal for real-time referenced biosensing in serum |
| title_full | Fiber-tip photonic crystal for real-time referenced biosensing in serum |
| title_fullStr | Fiber-tip photonic crystal for real-time referenced biosensing in serum |
| title_full_unstemmed | Fiber-tip photonic crystal for real-time referenced biosensing in serum |
| title_short | Fiber-tip photonic crystal for real-time referenced biosensing in serum |
| title_sort | fiber tip photonic crystal for real time referenced biosensing in serum |
| url | http://www.sciencedirect.com/science/article/pii/S2590137024001377 |
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