Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco Cheese
A Proficiency Test (PT) was conducted for Food Emergency Response Network (FERN) laboratories for quantitative assessment of Listeria monocytogenes (L. monocytogenes) in queso fresco cheese. The Moffett Proficiency Test Laboratory: MPTL (organizer) prepared test samples for each participating labora...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X24002321 |
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| author | Neha Singh Ravinder Reddy Karina Hettwer Kirstin Frost Matthew Kmet Steffen Uhlig |
| author_facet | Neha Singh Ravinder Reddy Karina Hettwer Kirstin Frost Matthew Kmet Steffen Uhlig |
| author_sort | Neha Singh |
| collection | DOAJ |
| description | A Proficiency Test (PT) was conducted for Food Emergency Response Network (FERN) laboratories for quantitative assessment of Listeria monocytogenes (L. monocytogenes) in queso fresco cheese. The Moffett Proficiency Test Laboratory: MPTL (organizer) prepared test samples for each participating laboratory with 10 CFU/g of L. monocytogenes. Statistical analysis was conducted to analyze test sample preparation and evaluate the performance of the quantitative method by utilizing participant’s data. Although individually inoculated, the samples were considered replicates based on the standard deviation values obtained from the Q/Hampel method. A similar observation was noted on pre- and postshipment data, which permitted a combined analysis as per Q/Hampel and ISO 5725-2 methods. Based on the results, it can be summarized that MPTL prepared homogenized and stable samples for L. monocytogenes enumeration in cheese. The participant results indicated that the repeatability of standard deviation, which reflects the scatter of results within a laboratory (participant), was mainly driven by the MPN procedure. Out of forty reported results from twenty laboratories, 85% were satisfactory. A few laboratories reported different MPN results (2.5% unsatisfactory, and 12.5% warning) indicating a lack of understanding in handling the MPN dilutions. Since BAM MPN-based method performance data for L. monocytogenes is not widely available in the literature, more PT events like these can help generate quantitative data for the evaluation of the MPN method. |
| format | Article |
| id | doaj-art-ea50c6b7aeff49f284b17d095be15d98 |
| institution | Kabale University |
| issn | 0362-028X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj-art-ea50c6b7aeff49f284b17d095be15d982025-02-06T05:11:02ZengElsevierJournal of Food Protection0362-028X2025-02-01883100448Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco CheeseNeha Singh0Ravinder Reddy1Karina Hettwer2Kirstin Frost3Matthew Kmet4Steffen Uhlig5Institute for Food Safety and Health, Illinois Institute of Technology, Bedford Park, Illinois, USADivision of Food Processing Science and Technology, U.S. Food and Drug Administration, Bedford Park, Illinois, USA; Corresponding author at: Division of Food Processing Science and Technology, U.S. Food and Drug Administration, Bedford Park, Illinois, USA.QuoData GmbH, Dresden, GermanyQuoData GmbH, Dresden, GermanyDivision of Food Processing Science and Technology, U.S. Food and Drug Administration, Bedford Park, Illinois, USAQuoData GmbH, Dresden, GermanyA Proficiency Test (PT) was conducted for Food Emergency Response Network (FERN) laboratories for quantitative assessment of Listeria monocytogenes (L. monocytogenes) in queso fresco cheese. The Moffett Proficiency Test Laboratory: MPTL (organizer) prepared test samples for each participating laboratory with 10 CFU/g of L. monocytogenes. Statistical analysis was conducted to analyze test sample preparation and evaluate the performance of the quantitative method by utilizing participant’s data. Although individually inoculated, the samples were considered replicates based on the standard deviation values obtained from the Q/Hampel method. A similar observation was noted on pre- and postshipment data, which permitted a combined analysis as per Q/Hampel and ISO 5725-2 methods. Based on the results, it can be summarized that MPTL prepared homogenized and stable samples for L. monocytogenes enumeration in cheese. The participant results indicated that the repeatability of standard deviation, which reflects the scatter of results within a laboratory (participant), was mainly driven by the MPN procedure. Out of forty reported results from twenty laboratories, 85% were satisfactory. A few laboratories reported different MPN results (2.5% unsatisfactory, and 12.5% warning) indicating a lack of understanding in handling the MPN dilutions. Since BAM MPN-based method performance data for L. monocytogenes is not widely available in the literature, more PT events like these can help generate quantitative data for the evaluation of the MPN method.http://www.sciencedirect.com/science/article/pii/S0362028X24002321MPNQuantitative dataProficiency test |
| spellingShingle | Neha Singh Ravinder Reddy Karina Hettwer Kirstin Frost Matthew Kmet Steffen Uhlig Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco Cheese Journal of Food Protection MPN Quantitative data Proficiency test |
| title | Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco Cheese |
| title_full | Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco Cheese |
| title_fullStr | Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco Cheese |
| title_full_unstemmed | Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco Cheese |
| title_short | Analysis of Method Performance for Quantitative Assessment of Listeria monocytogenes in Queso Fresco Cheese |
| title_sort | analysis of method performance for quantitative assessment of listeria monocytogenes in queso fresco cheese |
| topic | MPN Quantitative data Proficiency test |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X24002321 |
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