In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults
Pleurotus eryngii (PE), an edible mushroom rich in bioactive compounds, has been shown to exert immunomodulatory, anti-inflammatory, antioxidant, anti-carcinogenic, anti-microbial, antihepatotoxic and hypolipidemic activities, all important for the well-being of the ageing population. This study ass...
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Elsevier
2025-06-01
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| Series: | Human Nutrition & Metabolism |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666149725000179 |
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| author | Evangelia N. Kerezoudi Marigoula Vlassopoulou Evdokia K. Mitsou Georgia Saxami Georgios Koutrotsios Ioanna Taflampa Konstantinos C. Mountzouris Ignacio Rangel Robert J. Brummer Georgios I. Zervakis Vasiliki Pletsa Panagiotis Georgiadis Adamantini Kyriacou |
| author_facet | Evangelia N. Kerezoudi Marigoula Vlassopoulou Evdokia K. Mitsou Georgia Saxami Georgios Koutrotsios Ioanna Taflampa Konstantinos C. Mountzouris Ignacio Rangel Robert J. Brummer Georgios I. Zervakis Vasiliki Pletsa Panagiotis Georgiadis Adamantini Kyriacou |
| author_sort | Evangelia N. Kerezoudi |
| collection | DOAJ |
| description | Pleurotus eryngii (PE), an edible mushroom rich in bioactive compounds, has been shown to exert immunomodulatory, anti-inflammatory, antioxidant, anti-carcinogenic, anti-microbial, antihepatotoxic and hypolipidemic activities, all important for the well-being of the ageing population. This study assessed in vitro the prebiotic-like effects of multiple forms of this mushroom. An in vitro static batch fermentation was performed for 24 h with faecal inocula from five apparently healthy older adults in the presence of the following PE forms: whole food matrix (PEWS), in vitro digested (PEWSD) and rich in β-glucans extract (PEWSE). The changes in bacterial communities upon fermentation at family, genera and species level were detected via 16S rRNA Next Generation Sequencing and Quantitative real-time PCR. Short-chain fatty acids (SCFAs) were quantified using gas chromatography (GC), whereas other metabolites were analysed through ultra-high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). DEseq2 analysis indicated that PEWS presence exhibited the largest impact on faecal microbial families' and genera's abundance compared to negative (non-carbon source) and positive (inulin) controls. Only PEWS significantly increased Bifidobacterium spp. and F. prausnitzii populations, while all three forms robustly increased Bacteroides spp. levels and levels of butyrate, acetate and propionate acids. Statistical significance was set at p < 0.05. Overall, the findings highlight the beneficial effect of PE on intestinal health of older adults supporting its potential incorporation into innovative functional foods. However, additional in vivo studies are required to substantiate these findings before translating them into dietary guidelines or clinical applications. |
| format | Article |
| id | doaj-art-e9ff3a9bf8a94f5a847d293acb79ed13 |
| institution | DOAJ |
| issn | 2666-1497 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Human Nutrition & Metabolism |
| spelling | doaj-art-e9ff3a9bf8a94f5a847d293acb79ed132025-08-20T02:55:20ZengElsevierHuman Nutrition & Metabolism2666-14972025-06-014020031410.1016/j.hnm.2025.200314In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adultsEvangelia N. Kerezoudi0Marigoula Vlassopoulou1Evdokia K. Mitsou2Georgia Saxami3Georgios Koutrotsios4Ioanna Taflampa5Konstantinos C. Mountzouris6Ignacio Rangel7Robert J. Brummer8Georgios I. Zervakis9Vasiliki Pletsa10Panagiotis Georgiadis11Adamantini Kyriacou12Örebro University, School of Medical Sciences, Örebro, Sweden; Harokopio University, Department of Nutrition and Dietetics, Athens, GreeceHarokopio University, Department of Nutrition and Dietetics, Athens, Greece; National Hellenic Research Foundation, Institute of Chemical Biology, Athens, GreeceHarokopio University, Department of Nutrition and Dietetics, Athens, GreeceHarokopio University, Department of Nutrition and Dietetics, Athens, Greece; Institute of Technology of Agricultural Products, Hellenic Agricultural Organization – DIMITRA (ELGO-DIMITRA), Sof. Venizelou 1, 14123, Athens, GreeceAgricultural University of Athens, Department of Crop Science, Laboratory of General and Agricultural Microbiology, Athens, GreeceHarokopio University, Department of Nutrition and Dietetics, Athens, GreeceAgricultural University of Athens, Department of Nutritional Physiology and Feeding, Athens, GreeceÖrebro University, School of Medical Sciences, Örebro, SwedenÖrebro University, School of Medical Sciences, Örebro, SwedenAgricultural University of Athens, Department of Crop Science, Laboratory of General and Agricultural Microbiology, Athens, GreeceNational Hellenic Research Foundation, Institute of Chemical Biology, Athens, GreeceNational Hellenic Research Foundation, Institute of Chemical Biology, Athens, GreeceHarokopio University, Department of Nutrition and Dietetics, Athens, Greece; Corresponding author. Harokopio University of Athens, 176 71, Athens, Greece.Pleurotus eryngii (PE), an edible mushroom rich in bioactive compounds, has been shown to exert immunomodulatory, anti-inflammatory, antioxidant, anti-carcinogenic, anti-microbial, antihepatotoxic and hypolipidemic activities, all important for the well-being of the ageing population. This study assessed in vitro the prebiotic-like effects of multiple forms of this mushroom. An in vitro static batch fermentation was performed for 24 h with faecal inocula from five apparently healthy older adults in the presence of the following PE forms: whole food matrix (PEWS), in vitro digested (PEWSD) and rich in β-glucans extract (PEWSE). The changes in bacterial communities upon fermentation at family, genera and species level were detected via 16S rRNA Next Generation Sequencing and Quantitative real-time PCR. Short-chain fatty acids (SCFAs) were quantified using gas chromatography (GC), whereas other metabolites were analysed through ultra-high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). DEseq2 analysis indicated that PEWS presence exhibited the largest impact on faecal microbial families' and genera's abundance compared to negative (non-carbon source) and positive (inulin) controls. Only PEWS significantly increased Bifidobacterium spp. and F. prausnitzii populations, while all three forms robustly increased Bacteroides spp. levels and levels of butyrate, acetate and propionate acids. Statistical significance was set at p < 0.05. Overall, the findings highlight the beneficial effect of PE on intestinal health of older adults supporting its potential incorporation into innovative functional foods. However, additional in vivo studies are required to substantiate these findings before translating them into dietary guidelines or clinical applications.http://www.sciencedirect.com/science/article/pii/S2666149725000179Pleurotus eryngii mushroomsFaecal microbiotaMetabolitesβ-glucansElderlyPrebiotic |
| spellingShingle | Evangelia N. Kerezoudi Marigoula Vlassopoulou Evdokia K. Mitsou Georgia Saxami Georgios Koutrotsios Ioanna Taflampa Konstantinos C. Mountzouris Ignacio Rangel Robert J. Brummer Georgios I. Zervakis Vasiliki Pletsa Panagiotis Georgiadis Adamantini Kyriacou In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults Human Nutrition & Metabolism Pleurotus eryngii mushrooms Faecal microbiota Metabolites β-glucans Elderly Prebiotic |
| title | In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults |
| title_full | In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults |
| title_fullStr | In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults |
| title_full_unstemmed | In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults |
| title_short | In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults |
| title_sort | in vitro fermentation of whole matrix digested products and β glucan enriched extract of pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adults |
| topic | Pleurotus eryngii mushrooms Faecal microbiota Metabolites β-glucans Elderly Prebiotic |
| url | http://www.sciencedirect.com/science/article/pii/S2666149725000179 |
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